AXMI115 variant insecticidal gene and methods for its use

Patent metadata

Field	Value
Publication number	US-10647996-B2
Application number	US-201815960817-A
Country	US
Kind code	B2
Filing date	Apr 24, 2018
Priority date	Apr 5, 2011
Publication date	May 12, 2020
Grant date	May 12, 2020

Abstract

Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. The toxin coding sequences can be used in DNA constructs or expression cassettes for expression in plants and bacteria. Compositions include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, polynucleotide sequences and the toxin proteins encoded thereby are provided. Also provided are antibodies specifically binding to those amino acid sequences. In particular, the invention encompasses nucleotide sequences encoding fusion proteins, as well as biologically active variants and fragments thereof, wherein the fusion protein contains the C-terminal portion of SEQ ID NO:43. The fusion protein may also contain the N-terminal portion of SEQ ID NO:45. The invention also includes the nucleotide sequence of SEQ ID NO:47 and 1-14, or a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:2, 3, 7-14 and 47, including biologically active variants and fragments thereof.

First claim

That which is claimed:

1. A recombinant nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide having pesticidal activity, wherein said nucleotide sequence is the nucleotide sequence set forth in SEQ ID NO: 8; or
a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 22,
wherein the pesticidal activity of the polypeptide is improved or extended relative to the pesticidal activity of SEQ ID NO:43 or 45.

2. The recombinant nucleic acid molecule of claim 1 , wherein said nucleotide sequence is a synthetic sequence that has been designed for expression in a plant.

3. The recombinant nucleic acid molecule of claim 1 , wherein said nucleotide sequence is operably linked to a promoter capable of directing expression of said nucleotide sequence in a plant cell.

4. A vector comprising the recombinant nucleic acid molecule of claim 1 .

5. The vector of claim 4 , further comprising a nucleic acid molecule encoding a heterologous polypeptide.

6. A host cell that contains the recombinant nucleic acid of claim 1 .

7. The host cell of claim 6 that is a bacterial host cell.

8. The host cell of claim 6 that is a plant cell.

9. A transgenic plant comprising the host cell of claim 8 .

10. The transgenic plant of claim 9 , wherein said plant is selected from the group consisting of maize, sorghum, wheat, cabbage, sunflower, tomato, crucifers, peppers, potato, cotton, rice, soybean, sugarbeet, sugarcane, tobacco, barley, and oilseed rape.

11. A transgenic seed comprising the nucleic acid molecule of claim 1 .

12. A recombinant polypeptide having pesticidal activity, wherein said polypeptide comprises
the amino acid sequence of SEQ ID NO: 22.

13. The polypeptide of claim 12 further comprising heterologous amino acid sequences.

14. A composition comprising the polypeptide of claim 12 .

15. The composition of claim 14 , wherein said composition is selected from the group consisting of a powder, dust, pellet, granule, spray, emulsion, colloid, and solution.

16. The composition of claim 14 , wherein said composition is prepared by desiccation, lyophilization, homogenization, extraction, filtration, centrifugation, sedimentation, or concentration of a culture of bacterial cells.

17. The composition of claim 14 , comprising from about 1% to about 99% by weight of said polypeptide.

18. A method for controlling a lepidopteran pest population, said method comprising contacting said population with a pesticidally-effective amount of the polypeptide of claim 12 .

19. A method for killing a lepidopteran pest, said method comprising contacting said pest with, or feeding to said pest, a pesticidally-effective amount of the polypeptide of claim 12 .

20. A method for producing a polypeptide with pesticidal activity, said method comprising culturing the host cell of claim 6 under conditions in which the nucleic acid molecule encoding the polypeptide is expressed.

21. A plant or a plant cell having stably incorporated into its genome a DNA construct comprising a nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide having pesticidal activity, wherein the the nucleotide sequence is SEQ ID NO: 8 or a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 22,
wherein the pesticidal activity of the polypeptide is improved or extended relative to the pesticidal activity of SEQ ID NO:43 or 45.

22. A method for protecting a plant from a pest, said method comprising expressing in a plant or cell thereof a nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide having pesticidal activity, wherein the nucleotide sequence is SEQ ID NO: 8 or a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 22,
wherein the pesticidal activity of the polypeptide is improved or extended relative to the pesticidal activity of SEQ ID NO:43 or 45.

23. A method for increasing yield in a plant, said method comprising growing in a field a plant of or a seed thereof having stably incorporated into its genome a DNA construct comprising a nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide having pesticidal activity, wherein the nucleotide sequence is SEQ ID NO: 8 or a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 22,
wherein the pesticidal activity of the polypeptide is improved or extended relative to the pesticidal activity of SEQ ID NO:43 or 45.

Assignees

• Athenix Corp
• BASF Agricultural Solutions Seed US LLC

Inventors

• Desai Nalini Manoj
• Heinrichs Volker
• Lehtinen Duane

Classifications

• C07K2319/24

  containing a MBP (maltose binding protein)-tag · CPC title

• C07K2319/00

  Fusion polypeptide · CPC title

• C07K14/325

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title

• Y02A40/162

  Cross-Sectional Technologies · mapped topic