Cell sorter

Patent metadata

Field	Value
Publication number	US-10646899-B2
Application number	US-201815915633-A
Country	US
Kind code	B2
Filing date	Mar 8, 2018
Priority date	Sep 15, 2017
Publication date	May 12, 2020
Grant date	May 12, 2020

Abstract

According to one embodiment, a cell sorter includes a flow channel which supplies a sample liquid containing particles, a plurality of branch channels connected to the flow channel, an image sensor which has a pixel region covering the flow channel and the branch channels, a determination unit which determines the characteristics of the particles in the sample liquid from a measurement signal of the pixel region, and a separation unit guides the particles in the sample liquid to any of the branch channels based on the determination result of the determination unit.

First claim

What is claimed is:

1. A cell sorter, comprising:
a substrate;
a flow channel which supplies a sample liquid containing particles from one end side;
a plurality of branch channels which are connected to the other end side of the flow channel;
an image sensor which is provided in a surface region of the substrate, which is located beneath bottoms of the flow channel and the branch channels, and which has a pixel region covering the flow channel and the branch channels;
a determination unit which determines the characteristics of the particles in the sample liquid from a measurement signal of the pixel region of the portion of the flow channel of the image sensor; and
a separation unit which is provided adjacently to the connection of the flow channel and the branch channels and guides the particles in the sample liquid to any of the branch channels based on the determination result of the determination unit,
wherein the pixel region further covers the separation unit.

2. The cell sorter of claim 1 , further comprising an observation unit which observes transfer of the particles from the flow channel to the branch channels from the measurement signal of the image sensor.

3. The cell sorter of claim 1 , wherein
the determination unit has an optical filter which blocks a specific range or specific ranges of wavelength.

4. The cell sorter of claim 1 , wherein
the determination unit has a light source which irradiates the flow channel and the branch channels with light.

5. The cell sorter of claim 1 , wherein
the separation unit deflects the particles in a specific direction using dielectrophoresis, electrophoresis, ultrasonic wave, air pressure control, optical tweezer, magnetism.

6. The cell sorter of claim 1 , wherein
the separation unit has an electrode or electrodes.

7. The cell sorter of claim 1 , wherein
a plurality of cell sorter elements having the flow channel, the branch channels, the image sensor, the determination unit, and the separation unit are arranged in parallel, and the image sensor is commonly used for different cell sorter elements.

8. The cell sorter of claim 1 , further comprising
a return flow channel which is provided so as to connect one end side of the flow channel to the other end side of the flow channel, wherein the separation unit guides the particles in the sample liquid to any of the branch channels or the return flow channel based on the determination result of the determination unit.

9. The cell sorter of claim 1 , further comprising:
a plurality of rebranch channels connected to the branch channels;
a redetermination unit which determines the characteristics of the particles in the sample liquid from the measurement signal of the pixel region of the portion of the branch channels of the image sensor; and
a reseparation unit which is provided adjacently to the connection of the branch channels and the rebranch channels and guides the particles in the sample liquid to any of the rebranch channels based on the determination result of the redetermination unit.

10. A cell sorter, comprising:
a substrate;
a plurality of cell sorter elements arranged in series, the cell sorter elements each comprising:
a flow channel which supplies a sample liquid containing particles from one end side;
a plurality of branch channels whose one end is connected to the other end side of the flow channel;
an image sensor which is provided in a surface region of the substrate, which is located beneath bottoms of the flow channel and the branch channels, and which has a pixel region covering the flow channel and the branch channels;
a determination unit which determines the characteristics of the particles in the sample liquid from a measurement signal of the pixel region of the portion of the flow channel of the image sensor; and
a separation unit which is provided adjacently to the connection of the flow channel and the branch channels and guides the particles in the sample liquid in the flow channel to any of the branch channels based on the determination result of the determination unit;
wherein,
one end of the flow channel of the cell sorter element at the downstream side is connected to the other end of one of the branch channels of the cell sorter element at the upstream side,
the determination unit of each of the cell sorter elements determines different characteristic of particles, and
wherein the pixel region further covers the separation unit.

11. The cell sorter of claim 10 , wherein each of the cell sorter elements further comprises an observation unit which observes transfer of the particles from the flow channel to the branch channels from the measurement signal of the image sensor.

12. The cell sorter of claim 10 , wherein
the determination unit determines the fluorescent characteristic amount of the particles based on the measurement signal, and
the separation unit guides the particles to one of the branch channels based on the fluorescent characteristic amount.

13. The cell sorter of claim 10 , wherein
the determination unit has a light source which irradiates the flow channel and the branch channels with light.

14. A cell sorter, comprising:
a substrate
a flow channel section in which a cell sorter element having a first flow channel for supplying a sample liquid containing particles from one end side and a plurality of branch channels whose one end is connected to the other end side of the first flow channel is multi-stage arranged;
an image sensor which is provided in a surface region of the substrate, which is located beneath a bottom of the flow channel section, and which has a pixel region which covers the whole of the first flow channel and the branch channels or a pixel region which covers the flow channel section, for the cell sorter element;
a determination unit which determines the characteristics of particles in the sample liquid from a measurement signal of the pixel region of the portion of the first flow channel of the image sensor, for the cell sorter element; and
a separation unit which is provided adjacently to the connection of the first flow channel and the branch channels and guides particles in the sample liquid in the first flow channel to any of the branch channels based on the determination result of the determination unit, for the cell sorter element,
wherein the pixel region further covers the separation unit.

15. The cell sorter of claim 14 , further comprising an observation unit which observes transfer of the particles in the flow channel section from the measurement signal of the image sensor.

16. The cell sorter of claim 14 , wherein
the cell sorter element is provided in parallel at any of the stages.

17. The cell sorter of claim 14 , wherein
the flow channel section is multi-stage arranged by connecting one end of the first flow channel of the cell sorter element at the downstream side to the other end of one of the branch channels of the cell sorter element at the upstream side, and
the determination unit of each of the cell sorter elements determines different characteristic of particles.

18. The cell sorter of claim 17 , wherein
the determination unit determines the fluorescent characteristic amount of the particles based on the measurement signal,
the flow channel section in which the cell sorter element is multi-stage arranged has an upstream-side cell sorter element group including one or more cell sorter elements at the upstream side and a downstream-side cell sorter element group including one or more cell sorter elements at the dow

Assignees

• Toshiba Kk

Inventors

• IMAI Kaita
• Kousai Shouhei
• Akimoto Yosuke
• Nishigaki Michihiko
• Onozuka Yutaka
• Nagai Miyu

Classifications

• B01L2400/0439

  ultrasonic vibrations, vibrating piezo elements · CPC title

• B01L2400/0421

  electrophoretic flow · CPC title

• G02B5/20

  Filters (polarising elements G02B5/30) · CPC title

• B07C5/3427 Primary

  by changing or intensifying the optical properties prior to scanning, e.g. by inducing fluorescence under UV or x-radiation, subjecting the material to a chemical reaction · CPC title

• G01N15/1484

  microstructural devices · CPC title