Biochip storage wells

Patent metadata

Field	Value
Publication number	US-10646876-B2
Application number	US-201515534460-A
Country	US
Kind code	B2
Filing date	Dec 14, 2015
Priority date	Dec 15, 2014
Publication date	May 12, 2020
Grant date	May 12, 2020

Abstract

The present invention is directed to a cap for a biochip storage well. The cap comprises a resilient sealant layer capable, under the application of pressure, of forming a vapour-proof seal with a line contact interface formation extending around the perimeter of a biochip storage well.
An assay assembly comprising the cap of the invention and a biochip storage is disclosed. Methods of sealing a biochip storage well using a cap of the invention are also disclosed.

First claim

The invention claimed is:

1. An assay assembly comprising a biochip storage well having a base and one or more side walls, adapted to receive a biochip, with a line contact interface formation in the form of a knife-edge facing away from the base and extending along an upwardly facing perimeter of the one or more side walls and a cap comprising a resilient sealant layer, and comprising two opposing slide members, each defining a groove to enable the cap to slide onto a corresponding flange extending along the perimeter of the biochip storage well and thereby forming a vapour-proof seal with the line contact interface formation extending around the perimeter of the biochip storage well when the resilient sealant layer of the cap is applied, with a pressure, against the line contact interface formation and comprising a support member having a recess in which the biochip well sits, wherein the corresponding flange extending along the perimeter of the biochip storage well is formed by the support member.

2. The assay assembly according to claim 1 , wherein the pressure applied is in the range of 10-50 N.

3. The assay assembly according to claim 1 , wherein the cap further includes a component extending away from the sealant layer to allow the cap to be manipulated.

4. The assay assembly according to claim 3 , wherein the component extending away from the sealant layer is a hollow tube through which a pipette can be inserted.

5. The assay assembly according to claim 4 , further comprising a pierceable membrane at the base of the hollow tube.

6. The assay assembly according to claim 1 , wherein the resilient sealant layer is a polymer sealant.

7. The assay assembly according to claim 6 wherein the resilient sealant layer is polytetrafluoroethylene (PTFE), polychlorotrifluoroethylene (PCTFE) or polyethylene (PE).

8. The assay assembly according to claim 6 , wherein the resilient sealant layer is a nitrile layer.

9. The assay assembly according to claim 8 , wherein the nitrile sealant layer is 0.1 to 10 mm in thickness.

10. The assay assembly according to claim 1 , further comprising a hydrophobic layer, coated onto the resilient sealant layer.

11. The assay assembly according to claim 1 , wherein the cap is made from injection moulding.

12. The assay assembly according to claim 1 , wherein the cap comprises a plastic flat top section.

13. A method of sealing the assay assembly of claim 1 , comprising the steps of:
placing the cap onto the biochip storage well wall so that the resilient sealant layer is above the line contact interface formation;
applying external force onto the cap so that the cap closes the biochip storage well and the resilient sealant layer is pressed into the line contact interface formation, forming a vapour-proof seal, wherein the biochip storage well has a corresponding flange extending along the perimeter of the biochip storage well and wherein the cap slides onto the corresponding flange extending along the perimeter of the biochip storage well so as to form a vapour-proof seal with the line contact interface formation of the biochip storage well.

14. The method according to claim 13 wherein the pressure applied is in the range of 10-50 N.

15. The method according to claim 13 , wherein the vapour-proof seal provides less than 6% evaporation of the fluid within.

16. The method according to claim 13 , wherein the line contact interface formation is in the form of a knife-edge.

Assignees

• Randox Laboratories Ltd

Inventors

• Jackson Stuart
• Mcconnell Ivan
• Lamont John
• Fitzgerald Peter
• Wharry Marc

Classifications

• B01L2200/142

  Preventing evaporation · CPC title

• B01L2300/042

  Caps; Plugs · CPC title

• B01L2300/044

  pierceable, e.g. films, membranes · CPC title

• B01L2300/045

  whereby the whole cover is slidable · CPC title

• B01L9/52 Primary

  Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips · CPC title