Flow cell device for single cell analysis, and single cell analysis device

Patent metadata

Field	Value
Publication number	US-10646869-B2
Application number	US-201515544888-A
Country	US
Kind code	B2
Filing date	Feb 3, 2015
Priority date	Feb 3, 2015
Publication date	May 12, 2020
Grant date	May 12, 2020

Abstract

(1) Plural reaction chambers each including at least one cell capturing unit and at least one nucleic acid capturing unit connected via a flow channel, (2) a first liquid flow channel commonly connected to the plural reaction chambers on a first surface side, (3) plural second liquid flow channels connected to the plural reaction chambers in a one-on-one relationship on the first surface side, and (4) a third liquid flow channel commonly connected to the plural reaction chambers on a second surface side opposite to the first surface, are provided in a flow cell device for single cell analysis.

First claim

The invention claimed is:

1. A flow cell device for single cell analysis, comprising:
a plurality of reaction chambers each including one or more cell capturing units each configured to capture a single cell and one or more nucleic acid capturing units connected in a one-on-one relationship to the one or more cell capturing units via respective flow channels;
a first liquid flow channel that is commonly connected to the plurality of reaction chambers on a first surface side;
a plurality of second liquid flow channels that are connected to the plural reaction chambers in a one-on-one relationship on the first surface side; and
a third liquid flow channel that is commonly connected to the plural reaction chambers on a second surface side opposite to the first surface,
wherein each of the nucleic acid capturing units has a porous structure or a structure in which a plurality of beads are packed,
wherein each of the nucleic acid capturing units has immobilized therein one or more mRNA-capturing probes each including a DNA sequence for identifying the respective nucleic acid capturing unit thereof, and
wherein each of the cell capturing units has an opening with a first diameter configured to capture a single cell, and each of the nucleic acid capturing units includes the porous structure or the structure in which the plurality of beads are packed and having a second diameter larger than the first diameter.

2. The flow cell device for single cell analysis according to claim 1 , wherein the one or more mRNA-capturing probes each includes the DNA sequence for identifying the respective nucleic acid capturing unit thereof and a capturing sequence for capturing mRNA from the single cell captured by the respective cell capturing unit connected thereto.

3. The flow cell device for single cell analysis according to claim 1 , wherein all inner walls of the plurality of reaction chambers have been subjected to a hydrophilic treatment and an inner wall surface of an area of the first liquid flow channel that connects the plurality of reaction chambers has been subjected to a hydrophobic treatment.

4. The flow cell device for single cell analysis according to claim 1 , wherein each of the beads has a third diameter and each of the nucleic acid capturing units are connected by a plurality of lower flow channels to the third liquid flow channel, each of the lower flow channels having a fourth diameter smaller than the third diameter.

5. The flow cell device for single cell analysis according to claim 4 , wherein the cell capturing units are disposed on the first surface side and the plurality of the lower flow channels are disposed in a pore array sheet on the second surface side.

6. A flow cell device for single cell analysis, comprising:
a plurality of reaction chambers each including one or more cell capturing units each configured to capture a single cell and one or more nucleic acid capturing units connected in a one-on-one relationship to the one or more cell capturing units via respective flow channels;
a plurality of first liquid flow channels that are connected to the plurality of reaction chambers in a one-on-one relationship on a first surface side; and
a second liquid flow channel that is commonly connected to the plurality of reaction chambers on a second surface side opposite to the first surface,
wherein each of the nucleic acid capturing units has a porous structure or a structure in which a plurality of beads are packed,
wherein each of the nucleic acid capturing units has immobilized therein one or more mRNA-capturing probes each including a DNA sequence for identifying the respective nucleic acid capturing unit thereof, and
wherein each of the cell capturing units has an opening with a first diameter configured to capture a single cell, and each of the nucleic acid capturing units includes the porous structure or the structure in which the plurality of beads are packed and having a second diameter larger than the first diameter.

7. The flow cell device for single cell analysis according to claim 6 , wherein the one or more mRNA-capturing probes each includes the DNA sequence for identifying the respective nucleic acid capturing unit thereof and a capturing sequence for capturing mRNA from the single cell captured by the respective cell capturing unit connected thereto.

8. The flow cell device for single cell analysis according to claim 6 , wherein the first liquid flow channel is formed as an opening of the reaction chamber for exposing the cell capturing unit to the outside.

9. The flow cell device for single cell analysis according to claim 6 , wherein each of the beads has a third diameter and each of the nucleic acid capturing units are connected by a plurality of lower flow channels to the third liquid flow channel, each of the lower flow channels having a fourth diameter smaller than the third diameter.

10. The flow cell device for single cell analysis according to claim 9 , wherein the cell capturing units are disposed on the first surface side and the plurality of the lower flow channels are disposed in a pore array sheet on the second surface side.

11. A single cell analysis apparatus, comprising:
a stage on which a flow cell device for single cell analysis is placed, the flow cell device including:
a plurality of reaction chambers each including one or more cell capturing units each configured to capture a single cell and one or more nucleic acid capturing units connected in a one-on-one relationship to the one or more cell capturing units via respective flow channels,
a plurality first liquid flow channels connected to the plurality of reaction chambers in a one-on-one relationship on a first surface side, and
a second liquid flow channel commonly connected to the plurality of reaction chambers on a second surface side opposite to the first surface;

a stage controlling system configured to control a position of the stage;
a first introduction controller configured to individually control introduction of a first liquid into the plurality of first fluid flow channels; and
a discharge controller configured to control discharge of a second liquid from the second fluid flow channel,
wherein each of the nucleic acid capturing units has a porous structure or a structure in which a plurality of beads are packed, and
wherein each of the nucleic acid capturing units has immobilized therein one or more mRNA-capturing probes each including a DNA sequence for identifying the respective nucleic acid capturing unit thereof, and
wherein each of the cell capturing units has an opening with a first diameter configured to capture a single cell, and each of the nucleic acid capturing units includes the porous structure or the structure in which the plurality of beads are packed and having a second diameter larger than the first diameter.

12. The single cell analysis apparatus according to claim 11 , further comprising:
a third liquid flow channel that is commonly connected to the plurality of reaction chamber on the first surface side of the flow cell device;
a second introduction controller configured to control introduction of a third liquid into the third liquid flow channel; and
a second discharge controller configured to control discharge of the third liquid from the third liquid flow channel.

13. The single cell analysis apparatus according to claim 11 , further comprising:
an optical microscope for observing the flow cell device for single cell analysis, and correlating information acquired from an observation area to positional information acquired from the stage controlling system;
a DNA sequencer for analyzing a sequence of a nu

Assignees

• Hitachi Ltd

Inventors

• Shirai Masataka
• Sakai Tomoyuki

Classifications

• B01L2400/0688

  surface tension valves, capillary stop, capillary break · CPC title

• B01L2300/161

  Control and use of surface tension forces, e.g. hydrophobic, hydrophilic · CPC title

• C12N15/1003

  Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor · CPC title

• B01L2200/0668

  Trapping microscopic beads · CPC title

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title