Endophyte compositions and the methods for improvement of plant traits

Patent metadata

Field	Value
Publication number	US-10645938-B2
Application number	US-201715829844-A
Country	US
Kind code	B2
Filing date	Dec 1, 2017
Priority date	Mar 1, 2017
Publication date	May 12, 2020
Grant date	May 12, 2020

Abstract

This invention relates to methods and materials for modulating the characteristics of a plant, said plant having been heterologously disposed to an endophyte or a plurality of endophytes, or derived from a plant reproductive element heterologously disposed to an endophyte or a plurality of endophytes.

First claim

I claim:

1. A synthetic composition comprising a plant element and an endophyte, wherein the endophyte is heterologously disposed to the plant element, wherein the endophyte is a member of the genus Periconia and comprises at least one polynucleotide sequence at least 97% identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, wherein the region of alignment is at least 85% of the length of the query sequence, and wherein said synthetic composition is capable of providing an improved trait of agronomic importance as compared to a reference plant element not further comprising the endophyte.

2. The synthetic composition of claim 1 , wherein the plant element is of the genus Triticum and the improved trait of agronomic importance is selected from the group consisting of germination rate, dry shoot biomass, and yield.

3. The synthetic composition of claim 1 , wherein the plant element is of the genus Glycine and the improved trait of agronomic importance is selected from the group consisting of root length and root area.

4. The synthetic composition of claim 1 , wherein the plant element is of the genus Zea and the improved trait of agronomic importance is yield.

5. The synthetic composition of claim 1 , wherein the plant element is a seed.

6. The synthetic composition of claim 5 , wherein the seed is modified.

7. The synthetic composition of claim 1 , further comprising a formulation that comprises one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, an anticomplex agent, a fungicide, a nematicide, a bactericide, an insecticide, a herbicide, or any combination thereof.

8. The synthetic composition of claim 1 , wherein the synthetic composition is shelf-stable.

9. The synthetic composition of claim 1 , wherein the endophyte is a member of the genus Periconia as deposited under NRRL Culture Deposit No. 67466.

10. The synthetic composition of claim 1 , wherein the endophyte is a modified endophyte of the genus Periconia obtained from the endophyte as deposited under NRRL Culture Deposit No. 67466.

11. The synthetic composition of claim 1 , wherein the at least one polynucleotide sequence is 100% identical to SEQ ID NO: 32.

12. The synthetic composition of claim 1 , wherein the at least one polynucleotide sequence is 100% identical to SEQ ID NO: 33.

13. The synthetic composition of claim 1 , comprising a polynucleotide sequence that is at least 97% identical to SEQ ID NO: 32 and a polynucleotide sequence that is at least 97% identical to SEQ ID NO: 33.

14. The synthetic composition of claim 1 , comprising a polynucleotide sequence that is 100% identical to SEQ ID NO: 32 and a polynucleotide sequence that is 100% identical to SEQ ID NO: 33.

15. The synthetic composition of claim 1 , comprising a polynucleotide sequence that is at least 97% identical to SEQ ID NO: 33.

Assignees

• Indigo Ag Inc

Inventors

• Riley Raymond

Classifications

• A01H17/00 Primary

  Symbiotic or parasitic combinations including one or more new plants, e.g. mycorrhiza (lichens A01H15/00) · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title

• A01C1/06

  Coating or dressing seed · CPC title