Electromechanical lysis of bacterial pathogens using ion concentration polarization

Patent metadata

Field	Value
Publication number	US-10640744-B2
Application number	US-201715728860-A
Country	US
Kind code	B2
Filing date	Oct 10, 2017
Priority date	Jun 22, 2017
Publication date	May 5, 2020
Grant date	May 5, 2020

Abstract

Scalable, high throughput and power-efficient electromechanical lysis using low electric potential, which can be used for harvesting valuable intracellular biomolecules (DNA, RNA, and proteins) and metabolites (e.g., biodiesels, bioplastics, antibiotics, and antibodies), and for sterilizing large volume solutions (e.g. disinfection of bacterial contaminated drinking water). The method can be directly integrated with other microfluidic devices for all-in-one, fully integrated total-analysis systems for various bacterial (and cellular) studies and clinical applications.

First claim

What is claimed is:

1. A method of lysing a cell membrane comprising the steps:
a. directing a fluid stream containing cells in a channel comprising an inlet and an outlet and defined, at least in part, by at least a first ion exchange membrane and at least a second ion exchange membrane, wherein the ion exchange membranes are juxtaposed and characterized by the same charge;
b. applying an electric field across the channel at a voltage and duration sufficient to cause helical electroconvective vortex formation across the channel, thereby lysing the cell membranes of the cells;
c. collecting an output fluid stream comprising lysate from the outlet; and
d. isolating the lysate from the output fluid stream.

2. The method of claim 1 , wherein at least a first and at least a second juxtaposed ion exchange membrane are cationic exchange membranes.

3. The method of claim 1 , wherein at least a first and at least a second juxtaposed ion exchange membrane are anionic exchange membranes.

4. The method of claim 1 , wherein the channel is a microchannel.

5. The method of claim 1 , further comprising the step of concentrating the lysate.

6. The method of claim 5 , wherein at least one nonionic porous membrane is located at the outlet of the channel.

7. The method of claim 1 , wherein the electric field is created by an electrode and a ground each located external and parallel to the channel.

8. The method of claim 7 , wherein the electrode forms a second channel with at least a first ion exchange membrane and the ground forms a third channel with the at least a second ion exchange membranes.

9. The method of claim 8 , wherein the second and third channel are filled with an electrolyte solution.

10. The method of claim 1 , comprising a plurality or channels in parallel, each defined by an ion exchange membrane.

11. The method of claim 1 , wherein the cell membrane is a bacterial cell membrane.

12. The method of claim 11 , wherein isolating the lysate comprises isolating bacterial proteins, bacterial nucleic acids, or a combination thereof.

13. The method of claim 1 , wherein isolating the lysate comprises isolating intracellular biomolecules.

14. The method of claim 13 , wherein the intracellular biomolecules are selected from the group consisting of DNA, RNA, proteins, and bacterial metabolites, or a combination thereof.

15. The method of claim 14 , wherein the bacterial metabolites comprise biodiesels, bioplastics, antibiotics, and antibodies.

Assignees

• Massachusetts Inst Technology

Inventors

• Kim Minseok
• Han Jongyoon

Classifications

• C12N13/00

  Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves · CPC title

• C12N1/06 Primary

  Lysis of microorganisms · CPC title