Method for detecting circulating cells in superficial body fluids

Patent metadata

Field	Value
Publication number	US-10639012-B2
Application number	US-201615760453-A
Country	US
Kind code	B2
Filing date	Sep 19, 2016
Priority date	Sep 17, 2015
Publication date	May 5, 2020
Grant date	May 5, 2020

Abstract

The present invention relates to a method for measuring circulating cells in superficial body fluids by means of high-frequency-based device. The method can be used for detecting circulating cells in the fluids of an individual without the necessity of extracting a sample of the individual, being useful as a diagnostic tool and for monitoring the effectiveness of a treatment administered to an individual suffering from a viral, protozoal, fungal and/or bacterial disease.

First claim

The invention claimed is:

1. An in vitro method for detecting and quantifying circulating cells in superficial body fluids of a subject comprising
a) placing an ultrasound-based device in contact with a liquid sample isolated from a superficial body fluid of the subject for obtaining a ultrasound data of said liquid sample;
b) analyzing the ultrasound data obtained in step a) by a data processing system which provides information about cells and their concentration in the fluid, and
c) correlating the information obtained in step b) with the presence and amount of circulating cells in the individual,
wherein the ultrasound-based device operates at a central frequency in the range of 10-100 MHz and at a pulse duration between 25-1,000 ns, and at a wavelengths between 15 and 150 μm.

2. The method according to claim 1 , wherein the signal emitted by the ultrasound-based device operates at a central frequency of 20 MHz and/or at a pulse duration of 500 ns, and/or at a wavelength of 75 μm.

3. The method according to claim 1 , wherein the circulating cells are white blood cells, red blood cells or cancer cells.

4. The method according to claim 1 , wherein the ultrasound-based device comprises a transducer.

5. The method according to claim 1 , wherein the ultrasound data obtained in step a) is in the form of (i) collection of A-line data and/or of (ii) B-mode or C-mode type of 2D data or 3D data.

6. The method according to claim 1 , wherein the information about cells is cell size, cell concentration and/or cell viability.

7. The method according to claim 1 , wherein the superficial body fluid is selected from the group consisting of cerebrospinal fluid, blood, urine, pleural fluid, synovial fluid and pericardial fluid.

8. An in vitro method for the diagnosis of a viral, protozoal, fungal and/or bacterial disease in a subject or for identifying subjects who may be suffering from a viral, protozoal, fungal and/or bacterial disease comprising
a) quantifying circulating cells in superficial body fluids of the subject by a method according to claim 1 , and
b) correlating the amount of said circulating cells with the presence of a viral, protozoal, fungal and/or bacterial disease in the subject, wherein an amount of circulating cells higher than a reference value is indicative of a viral, protozoal, fungal and/or bacterial disease, or is indicative that the subject may be suffering from a viral, protozoal, fungal and/or bacterial disease.

9. An in vitro method for monitoring the effectiveness of a treatment administered to a subject suffering from a viral, protozoal, fungal and/or bacterial disease, comprising
a) quantifying circulating cells in a superficial body fluid of the subject before and after the treatment by a method according to claim 1 , and
b) correlating the amount of said circulating cells with the effectiveness of the treatment administered to said subject,
wherein an amount of circulating cells in the subject after the treatment lower than the amount of circulating cells before the treatment is indicative that the treatment is being effective.

10. An in vitro method according to claim 8 , wherein the viral, protozoal, fungal and/or bacterial disease is meningitis.

11. A non-invasive method for detecting and quantifying circulating cells in superficial body fluids comprising
a) placing an ultrasound-based device on the skin of the subject for obtaining a ultrasound data of the a superficial body fluid under the skin;
b) analyzing the ultrasound data obtained in step a) by a data processing system which provides information about cells and their concentration in the fluid, and
c) correlating the information obtained in step b) with the presence and amount of circulating cells in the subject,
wherein the ultrasound-based device operates at a central frequency in the range of 10-50 MHz, and at a pulse duration between 50-1,000 ns, and at a wavelength between 30 and 150 μm.

12. The method according to claim 11 , wherein the signal emitted by the ultrasound-based device operates at a central frequency of 20 MHz and/or at a pulse duration of 500 ns, and/or at a wavelength of 75 μm.

13. The method according to claim 11 , wherein the circulating cells are white blood cells, red blood cells or cancer cells.

14. The method according to claim 11 , wherein the ultrasound-based device comprises a transducer.

15. The method according to claim 11 , wherein the ultrasound data obtained in step a) is in the form of (i) collection of A-line data and/or of (ii) B-mode or C-mode type of 2D data or 3D data.

16. The method according to claim 11 , wherein the information about cells is cell size, cell concentration and/or cell viability.

17. The method according to claim 11 , wherein the superficial body fluid is selected from the group consisting of cerebrospinal fluid, blood, urine, pleural fluid, synovial fluid and pericardial fluid.

18. A method for the diagnosis of a viral, protozoal, fungal and/or bacterial disease in a subject or for identifying subjects who may be suffering from a viral, protozoal, fungal and/or bacterial disease, comprising
a) quantifying circulating cells in superficial body fluids of the subject by a method according to claim 11 , and
b) correlating the amount of said circulating cells with the presence of a viral, protozoal, fungal and/or bacterial disease in the subject, wherein an amount of circulating cells higher than a reference value is indicative of a viral, protozoal, fungal and/or bacterial disease, is indicative that the subject may be suffering from a viral, protozoal, fungal and/or bacterial disease.

19. Method for monitoring the effectiveness of a treatment administered to a subject suffering from a viral, protozoal, fungal and/or bacterial disease comprising
a) quantifying circulating cells in a superficial body fluid of the subject before and after the treatment by a method according to claim 11 , and
b) correlating the detection of said circulating cells with the effectiveness of the treatment administered to said subject,
wherein an amount of circulating cells in the subject after the treatment lower than the amount of circulating cells before the treatment is indicative that the treatment is being effective.

20. Method according to claim 18 , wherein the viral, protozoal, fungal and/or bacterial disease is meningitis.

Assignees

• Consejo Superior Investigacion
• Fund Para El Conocimiento Madri D
• Massachusetts Inst Technology

Inventors

• Elvira Segura Luis
• Martinez Graullera Oscar
• Kant Shukla Shiva
• Montero Francisco
• Jimenez Javier
• Butterworth Ian Richard
• Anthony Brian
• Lee Haeseon John
• Castro Gonzalez Carlos

Classifications

• G01N15/0211

  Investigating a scatter or diffraction pattern · CPC title

• G01N2291/02466

  Biological material, e.g. blood · CPC title

• G01N29/032

  by measuring attenuation of acoustic waves · CPC title

• G16H50/30

  for calculating health indices; for individual health risk assessment · CPC title

• G01N2015/0687

  in solutions, e.g. non volatile residue · CPC title