Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof

Patent metadata

Field	Value
Publication number	US-10638734-B2
Application number	US-90749510-A
Country	US
Kind code	B2
Filing date	Oct 19, 2010
Priority date	Jan 5, 2004
Publication date	May 5, 2020
Grant date	May 5, 2020

Abstract

The present invention relates generally to mammalian multipotent adult stem cells (MASC), and more specifically to methods for obtaining, maintaining and differentiating MASC to cells of multiple tissue types. Uses of MASC in the therapeutic treatment of disease are also provided.

First claim

What is claimed is:

1. A method for identifying an agent that affects a desired cellular response, the method comprising:
a) contacting an isolated culture-expanded cell population with a desired agent, wherein the cells of the population are not embryonic stem cells, embryonic germ cells, or germ cells, express oct4 and/or telomerase and are not tumorigenic, and have undergone at least 10-40 cell doublings in culture prior to being contacted with the agent;
b) determining whether said agent affects said cellular response in said cells of the population.

2. A method according to claim 1 , wherein the cells of the population express telomerase.

3. A method according to claim 1 , wherein the cells of the population express oct4.

4. A method according to claim 2 , wherein the cells of the population express oct4.

5. A method according to any one of claims 1 - 3 , wherein the cells of the population can differentiate into cell types of at least two of the endodermal, ectodermal, and mesodermal embryonic lineages.

6. A method according to claim 5 , wherein the cells of the population can differentiate into at least one cell type of each of the endodermal, ectodermal and mesodermal embryonic lineages.

7. A method according to any one of claims 1 - 3 , wherein the agent is a biological or pharmacological agent.

8. A method according to claim 7 , wherein said biological agent is a cytokine, chemokine, or growth factor.

9. A method according to any one of claims 1 - 3 , wherein said cellular response is gene expression.

10. The method of claim 1 , wherein the cells that are non-embryonic stem cells, embryonic germ cells, or germ cells have undergone about 30 cell doublings in culture prior to step (a).

11. The method of claim 1 , wherein the cells that are not embryonic stem cells, embryonic germ cells, or germ cells, have undergone about 40 cell doublings in culture prior to step (a).

12. A method for identifying an agent that affects a desired cellular response, the method comprising:
a) contacting an isolated culture-expanded cell population with a desired agent, wherein the cells of the population are not embryonic stem cells, embryonic germ cells, or germ cells, are derived from human bone marrow, express oct4 and/or telomerase, and have a normal karyotype, and have undergone at least 10-40 cell doublings in culture prior to being contacted with the agent; and
b) determining whether said agent affects said cellular response in said cells of the population.

13. A method according to claim 12 , wherein the cells of the population express telomerase.

14. A method according to claim 12 , wherein the cells of the population express oct4.

15. A method according to claim 13 , wherein the cells of the population express oct4.

16. A method according to any one of claims 12 - 15 , wherein the cells of the population can differentiate into cell types of at least two of the endodermal, ectodermal and mesodermal embryonic lineages.

17. A method according to claim 16 , wherein the cells of the population can differentiate into at least one cell type of each of the endodermal, ectodermal and mesodermal embryonic lineages.

18. The method of claim 12 , wherein the cells that are not non-embryonic stem cells, embryonic germ cells, or germ cells have undergone about 30 cell doublings in culture prior to step (a).

19. The method of claim 12 , wherein the cells that are not embryonic stem cells, embryonic germ cells, or germ cells, have undergone about 40 cell doublings in culture prior to step (a).

20. The method according to claim 1 , where the cells of the population are derived from human bone marrow.

Assignees

• Furcht Leo T
• Verfaillie Catherine M
• Reyes Morayma
• Abt Holding Co
• Univ Minnesota

Inventors

• Furcht Leo T
• Verfaillie Catherine M
• Reyes Morayma

Classifications

• A01K67/0271 Primary

  Chimeric vertebrates, e.g. comprising exogenous cells · CPC title

• A01K2227/105

  Murine · CPC title

• C12N15/873

  Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos · CPC title