Method for processing a liquid medium comprising cells

Patent metadata

Field	Value
Publication number	US-10632476-B2
Application number	US-201816028636-A
Country	US
Kind code	B2
Filing date	Jul 6, 2018
Priority date	Jan 27, 2014
Publication date	Apr 28, 2020
Grant date	Apr 28, 2020

Abstract

A method for processing an initial liquid medium including cells in an initial concentration to obtain a product liquid medium including cells in a product concentration includes supplying the initial medium to a separator via a first supply system, separating the initial medium into various constituents including the product medium, extracting the product medium from the separator via a first outlet system, measuring a physical parameter related to the concentration of the cells in the initial medium using a sensor, and controlling at least one process parameter as a function of the physical parameter. During the step of separating the initial medium, a solution is supplied to the separator at a flow rate via a second supply system. The flow rate of the solution is determined based on the at least one process parameter, and is reduced when the concentration of the cells measured in the initial medium increases.

First claim

The invention claimed is:

1. A method for processing an initial liquid medium composing cells in an initial concentration in order to obtain a product liquid medium comprising cells in a product concentration, the method comprising the following steps:
supplying the initial liquid medium to a separator via a first supply system,
in the separator, separating the initial liquid medium into various constituents including the product liquid medium,
extracting the product liquid medium from the separator via a first outlet system,
measuring a physical parameter related to the concentration of the cells in the initial liquid medium using a sensor, and
controlling at least one process parameter as a function of the physical parameter measured by the sensor,
wherein, during the step of separating the initial liquid medium into various constituents, a solution is supplied to the separator at a flow rate via a second supply system,
the flow rate of the solution in the second supply system during its supply to the separator being determined based on the at least one process parameter, and the flow rate of the solution in the second supply system being reduced when the concentration of the cells measured in the initial liquid medium increases.

2. The method according to claim 1 , wherein the initial liquid medium is supplied at a flow rate in the first supply system to the separator determined based on the at least one process parameter.

3. The method according to claim 2 , wherein the flow rate of the initial liquid medium is indirectly proportional to the initial concentration of the cells in the initial liquid medium in the first supply system.

4. The method according to claim 2 , wherein the product liquid medium is extracted at a flow rate in the first outlet system from the separator determined based on the at least one process parameter.

5. The method according to claim 4 , wherein the flow rate of the product liquid medium is directly proportional to the initial concentration of the cells in the initial liquid medium in the first supply system.

6. The method according to claim 1 , wherein the product liquid medium is extracted at a flow rate in the first outlet system from the separator determined based on the at least one process parameter.

7. The method according to claim 6 , wherein the flow rate of the product liquid medium is directly proportional to the initial concentration of the cells in the initial liquid medium in the first supply system.

8. The method according to claim 1 , wherein the step of separating the initial liquid medium into various constituents comprises centrifuging the initial liquid medium.

9. The method according to claim 8 , wherein the initial liquid medium is centrifuged at a rotational speed determined based on the at least one process parameter such that the rotational speed is indirectly proportional to the initial concentration of the cells in the initial liquid medium in the first supply system.

10. The method according to claim 1 , wherein, during the step of controlling the at least one process parameter, the product concentration of the cells in the product liquid medium is within a predefined concentration range.

11. The method according to claim 1 , further comprising measuring a physical parameter elated to the product concentration of the cells in the product liquid medium using another sensor.

12. The method according to claim 1 , wherein the liquid medium is blood comprising blood cells.

Assignees

• Fresenius Kabi Deutschland Gmbh

Inventors

• Meisberger Artur
• Fahrendorff Melanie
• Brinkmann Michael
• Biehl Martin

Classifications

• A61M1/3616

  Batch-type treatment · CPC title

• A61M1/3692

  Washing or rinsing blood or blood constituents · CPC title

• B04B11/02

  Continuous feeding or discharging; Control arrangements therefor · CPC title

• C07D403/04

  directly linked by a ring-member-to-ring-member bond · CPC title

• B04B9/10 Primary

  Control of the drive; Speed regulating {(for solid bowl screw centrifuges B04B1/2016)} · CPC title