Lactobacillus paracasei strain
US-12152232-B2 · Nov 26, 2024 · US
Use of uric acid for culturing bacteria sensitive to oxygen tension
US10626421B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10626421-B2 |
| Application number | US-201515304887-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 21, 2015 |
| Priority date | Apr 23, 2014 |
| Publication date | Apr 21, 2020 |
| Grant date | Apr 21, 2020 |
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- Title
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Abstract
Official abstract text for this publication.
The present invention relates to a method for cultivating in vitro in an acellular culture medium, bacteria for which the growth is sensitive to oxygen content, said bacterium being selected from anaerobic bacteria and intracellular micro-aerophilic bacteria, characterized in that uric acid is added into said acellular culture medium, and said bacterium is cultivated in said culture medium in the presence of oxygen.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for cultivating, in vitro, in an acellular culture medium, a strict anaerobic bacterium, wherein said strict anaerobic bacterium is cultivated in said acellular culture medium under an atmosphere comprising oxygen gas, and wherein uric acid at a concentration of at least 0.1 g/L is added to said acellular culture medium. 2. The method according to claim 1 , wherein the uric acid is added at a concentration of at least 0.2 g/L. 3. The method according to claim 1 , wherein the uric acid is added without any additional antioxidant compound in said acellular culture medium. 4. The method according to claim 1 , wherein the uric acid is added with at least one additional antioxidant compound in said culture medium. 5. The method according to claim 4 , wherein the uric acid is added in combination with at least one additional antioxidant compound selected from the group consisting of ascorbic acid and glutathion. 6. The method according to claim 4 , wherein the uric acid is added in combination with ascorbic acid and glutathion. 7. The method according to claim 1 , wherein said acellular medium is selected from the group consisting of: an axenic medium consisting of chemical or biological substances qualitatively and quantitatively defined, and a medium comprising an extract of milled or lysed material of pluricellular tissue. 8. The method according to claim 1 , wherein said strict anaerobic bacterium is an extracellular strict anaerobic bacterium, and wherein said culture medium comprises a member selected from the group consisting of an extract of milled or lysed material of pluricellular tissue, an enzymatic digested material of casein, soya, and/or animal tissue, a peptone, a yeast extract, a sugar, a salt NaCl, Na 2 PO 4 , and a combination thereof. 9. The method according to claim 1 , wherein said strict anaerobic bacterium is cultivated under an atmosphere of ambient air.
Assignees
Inventors
Classifications
- C12N1/20Primary
Bacteria; Culture media therefor · CPC title
Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound (C12N1/34 takes precedence) · CPC title
- C12P5/023Primary
Methane · CPC title
Processes involving microorganisms of different genera in the same process, simultaneously · CPC title
Cross-Sectional Technologies · mapped topic
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- What does patent US10626421B2 cover?
- The present invention relates to a method for cultivating in vitro in an acellular culture medium, bacteria for which the growth is sensitive to oxygen content, said bacterium being selected from anaerobic bacteria and intracellular micro-aerophilic bacteria, characterized in that uric acid is added into said acellular culture medium, and said bacterium is cultivated in said culture medium in t…
- Who is the assignee on this patent?
- Fond Mediterranee Infection, Univ Aix Marseille, Centre Nat Rech Scient, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification C12N1/20. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 21 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).