Maize cytoplasmic male sterility (CMS) S-type restorer gene Rf3

Patent metadata

Field	Value
Publication number	US-10626412-B2
Application number	US-201815887715-A
Country	US
Kind code	B2
Filing date	Feb 2, 2018
Priority date	Dec 31, 2013
Publication date	Apr 21, 2020
Grant date	Apr 21, 2020

Abstract

A newly identified protein that is encoded by a polynucleotide sequence associated with cytoplasmic male sterility restorer activity (Rf3) is described. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to restore cytoplasmic male sterility in plants. Further applications of the newly identified polynucleotide sequence associated with cytoplasmic male sterility restorer activity include a mutation (rf3) which results in cytoplasmic male sterility. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to result in cytoplasmic male sterility in plants. Methods for detecting the cytoplasmic male sterility restorer (Rf3) and the cytoplasmic male sterility (rf3) gene sequences are further described.

First claim

What is claimed is:

1. A synthetic polynucleotide encoding a fusion polypeptide comprising cytoplasmic male sterile activity, wherein the synthetic polynucleotide encodes a fusion polypeptide comprising an amino acid sequence having at least 97.5% sequence identity to the amino acid sequence of SEQ ID NO: 3, and further encoding an amino acid motif comprising the amino acid sequence of SEQ ID NO: 1.

2. The synthetic polynucleotide of claim 1 , wherein the synthetic polynucleotide encoding the amino acid motif comprises the nucleotide sequence comprising the sequence of SEQ ID NO: 2.

3. The synthetic polynucleotide of claim 1 , wherein the synthetic polynucleotide is operably linked to a promoter, wherein the promoter is functional in plants.

4. The synthetic polynucleotide of claim 3 , wherein the promoter is a cytoplasmic male sterile promoter.

5. A cell comprising the synthetic polynucleotide of claim 1 .

6. A vector comprising the synthetic nucleic acid sequence polynucleotide of claim 1 .

7. A cell comprising the vector of claim 6 .

8. A method for restoring fertility to a cytoplasmic male sterile plant, the method comprising the steps of:
a) transforming the cytoplasmic male sterile plant with the synthetic polynucleotide of claim 1 ;
b) integrating the synthetic polynucleotide into the genome of the cytoplasmic male sterile plant; and,
c) expressing the synthetic polynucleotide, wherein expression of the synthetic polynucleotide restores fertility to the cytoplasmic male sterile plant.

9. A method for altering plant morphology in a cytoplasmic male sterile plant, the method comprising the steps of:
a) transforming the cytoplasmic male sterile plant with the synthetic polynucleotide of claim 1 ;
b) integrating the synthetic polynucleotide into the genome of the cytoplasmic male sterile plant; and
c) expressing the synthetic polynucleotide, wherein expression of the the synthetic polynucleotide alters plant morphology in the cytoplasmic male sterile plant.

Assignees

• Dow Agrosciences Llc

Inventors

• Ren Ruihua
• Nagel Bruce A
• Ye Liang
• Gao Yanxin Star
• Gibson Ryan
• Paulraj Sushmitha
• Mansfield Tyler
• Mammadov Jafar
• Kumpatla Siva P
• Thompson Steven A

Classifications

• C12N15/8289

  Male sterility · CPC title

• C07K14/415

  from plants · CPC title

• C12N15/8287 Primary

  for fertility modification, e.g. apomixis · CPC title

• A01H1/02

  Methods or apparatus for hybridisation; Artificial pollination {; Fertility} · CPC title

• A01H1/023

  Male sterility · CPC title