Identification of amino acids or short peptides
US-2025216398-A1 · Jul 3, 2025 · US
Method for specific cleavage of C alpha-C bond and side chain of protein and peptide, and method for determining amino acid sequence
US10626141B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10626141-B2 |
| Application number | US-201715594461-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 12, 2017 |
| Priority date | May 16, 2016 |
| Publication date | Apr 21, 2020 |
| Grant date | Apr 21, 2020 |
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- Title
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Abstract
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The present invention provides a method for specifically cleaving a Cα-C bond of a peptide backbone and/or a side chain of a protein and a peptide, and a method for determining amino acid sequences of protein and peptide. A method for specifically cleaving a Cα-C bond of a peptide backbone and/or a side chain bond of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of at least one hydroxynitrobenzoic acid selected from the group consisting of 3-hydroxy-2-nitrobenzoic acid, 4-hydroxy-3-nitrobenzoic acid, 5-hydroxy-2-nitrobenzoic acid, 3-hydroxy-5-nitrobenzoic acid, and 4-hydroxy-2-nitrobenzoic acid. A method for determining an amino acid sequence of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of the above specific hydroxynitrobenzoic acid to specifically cleave a Cα-C bond of a peptide backbone and/or a side chain bond, and analyzing generated fragment ions by mass spectrometry.
First claim
Opening claim text (preview).
What is claimed is: 1. A method for specifically cleaving a Cα-C bond of a peptide backbone and/or a side chain bond of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of at least one hydroxynitrobenzoic acid selected from the group consisting of: 3-hydroxy-2-nitrobenzoic acid: 4-hydroxy-3-nitrobenzoic acid: 5-hydroxy-2-nitrobenzoic acid: 3-hydroxy-5-nitrobenzoic acid: and 4-hydroxy-2-nitrobenzoic acid: to specifically cleave the Cα-C bond of the peptide backbone and/or the side chain bond, wherein the hydroxynitrobenzoic acid promotes the specific cleavage of the Cα-C bond of the peptide backbone and/or the side chain bond. 2. A method for determining an amino acid sequence of a protein or a peptide, comprising: irradiating a protein or a peptide with laser light in the presence of at least one hydroxynitrobenzoic acid selected from the group consisting of: 3-hydroxy-2-nitrobenzoic acid: 4-hydroxy-3-nitrobenzoic acid: 5-hydroxy-2-nitrobenzoic acid: 3-hydroxy-5-nitrobenzoic acid: and 4-hydroxy-2-nitrobenzoic acid: to specifically cleave a Cα-C bond of a peptide backbone and/or a side chain bond; and analyzing generated fragment ions by mass spectrometry, wherein the hydroxynitrobenzoic acid promotes the specific cleavage of the Cα-C bond of the peptide backbone and/or the side chain bond. 3. A method for determining an amino acid sequence of a protein or a peptide, comprising: irradiating a protein or a peptide with laser light in the presence of at least one hydroxynitrobenzoic acid selected from the group consisting of 3-hydroxy-2-nitrobenzoic acid, 4-hydroxy-3-nitrobenzoic acid, 5-hydroxy-2-nitrobenzoic acid, 3-hydroxy-5-nitrobenzoic acid, and 4-hydroxy-2-nitrobenzoic acid as a matrix to specifically cleave a Cα-C bond of a peptide backbone and/or a side chain bond; and analyzing generated fragment ions by MALDI mass spectrometry, wherein the hydroxynitrobenzoic acid promotes the specific cleavage of the Cα-C bond of the peptide backbone and/or the side chain bond. 4. The method for determining an amino acid sequence of a protein or a peptide according to claim 2 , wherein as the generated fragment ions, a-series ion species and/or x-series ion species are analyzed. 5. The method for determining an amino acid sequence of a protein or a peptide according to claim 2 , wherein as the generated fragment ions, a-series ion species and d-series ion species are analyzed.
Assignees
Inventors
Classifications
Methods of protein analysis involving laser desorption ionisation mass spectrometry · CPC title
in ortho-position to the carboxyl group, e.g. nitro-salicylic acids · CPC title
by a photon beam, photo-dissociation · CPC title
- C07K1/128Primary
sequencing · CPC title
the carbon skeleton being further substituted by singly-bound oxygen atoms · CPC title
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- What does patent US10626141B2 cover?
- The present invention provides a method for specifically cleaving a Cα-C bond of a peptide backbone and/or a side chain of a protein and a peptide, and a method for determining amino acid sequences of protein and peptide. A method for specifically cleaving a Cα-C bond of a peptide backbone and/or a side chain bond of a protein or a peptide, comprising irradiating a protein or a peptide with las…
- Who is the assignee on this patent?
- Shimadzu Corp, National Univ Of Corporation Hiroshima Univ
- What technology area does this patent fall under?
- Primary CPC classification C07K1/128. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 21 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).