Methods and compositions for hybrid microfluidic devices integrated with nano-biosensors

Patent metadata

Field	Value
Publication number	US-10620200-B2
Application number	US-201514725689-A
Country	US
Kind code	B2
Filing date	May 29, 2015
Priority date	May 29, 2014
Publication date	Apr 14, 2020
Grant date	Apr 14, 2020

Abstract

Certain embodiments are directed to paper/polymer hybrid microfluidic devices integrated with nano-biosensors for pathogen detection and infectious disease diagnosis.

First claim

The invention claimed is:

1. A paper-polymer hybrid microfluidic device comprising:
(a) a microfluidic support having at least two separate layers,
(i) a top layer having at least one microchannel formed in the top layer, the at least one microchannel comprises an inlet reservoir, and an outlet connected by a conduit, wherein the top layer comprises a polymer;
(ii) a bottom layer position below the top layer and bonded to the top layer and the bottom layer forming a cylindrical detection microwell, the detection microwell having an open top part in fluid communication with the at least one microchannel of the top layer, a closed bottom part positioned 1 millimeters (mm) to 4 mm below the top layer, and a horizontal cross-sectional diameter 0.5 mm to 3 mm, wherein the bottom layer comprises a polymer;

(b) a paper insert nanosensor positioned on the closed bottom part of the cylindrical detection microwell forming a floor of the microwell, the paper insert nanosensor having an adsorbed nanosensor complex comprising a fluorescently labeled aptamer probe reversibly complexed with a fluorescence quenching moiety selected from the group consisting of graphene oxide, graphene, and carbon nanoparticles, wherein the paper insert has a thickness of between 0.05 mm to 2 mm and a pore diameter of between 5 micrometers (μm) to 15 μm, and wherein the bottom layer is thicker than the paper insert; and
(c) a support layer positioned below the closed bottom part of the cylindrical detection microwell forming a floor of the cylindrical detection microwell of the second layer.

2. The paper-polymer hybrid microfluidic device of claim 1 , wherein the paper-polymer hybrid microfluidic device comprises a plurality of cylindrical detection microwells.

3. The paper-polymer hybrid microfluidic device of claim 2 , wherein the plurality of cylindrical detection microwells are arrange in an array.

4. The paper-polymer hybrid microfluidic device of claim 1 , wherein the polymer of the top layer is polydimethylsiloxane (PDMS).

5. The paper-polymer hybrid microfluidic device of claim 1 , wherein the polymer of the bottom layer is polydimethylsiloxane (PDMS).

6. The paper-polymer hybrid microfluidic device of claim 1 , wherein the support layer is glass, or PDMS.

7. The paper-polymer hybrid microfluidic device of claim 1 , wherein the paper is porous chromatography paper.

8. The paper-polymer hybrid microfluidic device of claim 1 , wherein the fluorescently labeled aptamer probe is bound to a pathogen.

9. The paper-polymer hybrid microfluidic device of claim 8 , wherein the pathogen is a bacteria or virus.

10. The paper-polymer hybrid microfluidic device of claim 1 , wherein the paper insert nanosensor comprises two or more distinct fluorescently labeled aptamer probes.

11. The paper-polymer hybrid microfluidic device of claim 1 wherein the bottom layer comprises two or more cylindrical detection microwells, each of the two or more cylindrical detection microwells comprising a distinct paper insert nanosensor.

Assignees

• Li Xiujun
• Dou Maowei
• Univ Texas

Inventors

• Li Xiujun
• Dou Maowei

Classifications

• G01N2333/22

  from Neisseriaceae (F), e.g. Acinetobacter · CPC title

• B01L2400/0409

  centrifugal forces · CPC title

• B01L3/5023

  with a sample being transported to, and subsequently stored in an absorbent for analysis · CPC title

• B01L2300/069

  Absorbents; Gels to retain a fluid · CPC title

• B01L2300/0864

  comprising only one inlet and multiple receiving wells, e.g. for separation, splitting · CPC title