Sensing sensor and sensing method

Patent metadata

Field	Value
Publication number	US-10620199-B2
Application number	US-201715438766-A
Country	US
Kind code	B2
Filing date	Feb 22, 2017
Priority date	Feb 29, 2016
Publication date	Apr 14, 2020
Grant date	Apr 14, 2020

Abstract

A sensing sensor includes a wiring board, a piezoelectric resonator, and a gel-like protective agent. The piezoelectric resonator has one surface side on which an adsorbing film is formed. The adsorbing film is constituted of biomolecules. The protective agent is disposed so as to cover a surface of the adsorbing film. The protective agent is configured to suppress an inactivation of the biomolecules. The channel forming member is disposed so as to cover a region of the one surface side of the wiring board including the piezoelectric resonator. The channel forming member includes an injection port of the sample solution. The flow passage is disposed between the wiring board and the channel forming member. The flow passage is configured to allow the sample solution supplied to the injection port to flow from one end side to another end side on the one surface side of the piezoelectric resonator.

First claim

What is claimed is:

1. A sensing sensor comprising:
a wiring board that includes a connection terminal and a depressed portion, wherein the connection terminal is formed on one side of the wiring board for connection to a measuring device for measuring an oscillation frequency, the depressed portion is formed on one surface side of the wiring board;
a piezoelectric resonator that includes a piezoelectric piece, an excitation electrode and an extraction electrode on the piezoelectric piece, the piezoelectric resonator being secured to the wiring board such that the piezoelectric resonator covers the depressed portion and a vibrating region is opposed to the depressed portion, the excitation electrode being electrically connected to the connection terminal, the piezoelectric resonator having one surface side on which an adsorbing film constituted of biomolecules for adsorbing a sensing object in a sample solution is formed, and a blocking film that inhibits adhesion of the sensing object to a surface is arranged in a region other than a region on which the adsorbing film is formed and on the extraction electrode;
a protective agent having a high viscosity, disposed to cover a surface of the adsorbing film for suppressing an inactivation of the biomolecules;
a channel forming member disposed to cover a region of the one surface side of the wiring board including the piezoelectric resonator, the channel forming member including an injection port of the sample solution; and
a flow passage that is disposed between the wiring board and the channel forming member and allowing the sample solution supplied to the injection port to flow from one end side to another end side on the one surface side of the piezoelectric resonator.

2. The sensing sensor according to claim 1 , comprising:
an effluent channel disposed on a downstream side of the flow passage, the effluent channel discharging the sample solution in the flow passage by a capillarity action;
a capillary member disposed on a downstream side of the effluent channel to contact the sample solution flowing through the effluent channel and allow the sample solution to flow by the capillarity action; and
an absorbing member disposed on a downstream side of the capillary member to absorb the sample solution flowing through the capillary member.

3. The sensing sensor according to claim 2 , wherein
the protective agent is constituted of at least one of glycerin and sugar.

4. The sensing sensor according to claim 1 , wherein
the protective agent is constituted of at least one of glycerin and sugar.

Assignees

• Nihon Dempa Kogyo Co

Inventors

• Shinobu Wakako

Classifications

• G01N29/022

  Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices (microdevices per se B81B) · CPC title

• G01N2291/0256

  Adsorption, desorption, surface mass change, e.g. on biosensors · CPC title

• G01N29/222

  Constructional or flow details for analysing fluids (optoacoustic fluid cells G01N29/2425) · CPC title

• G01N2291/0426

  Bulk waves, e.g. quartz crystal microbalance, torsional waves · CPC title

• G01N2291/0255

  (Bio)chemical reactions, e.g. on biosensors · CPC title