Apparatus and processes for photosynthetic activity measurement and mapping

Patent metadata

Field	Value
Publication number	US-10620175-B2
Application number	US-201615153789-A
Country	US
Kind code	B2
Filing date	May 13, 2016
Priority date	May 13, 2015
Publication date	Apr 14, 2020
Grant date	Apr 14, 2020

Abstract

A method for determining chlorophyll content of a plant comprises capturing a first image comprising light transmitted through a leaf of a plant; capturing a second image comprising light reflected from the leaf of the plant; estimating, from a plurality of pixels in the first image, a transmissive chlorophyll concentration value of the leaf; estimating a reflectance chlorophyll concentration value for the leaf from a plurality of pixels in the second image using bidirectional reflectance parameters for which a variance of the reflectance chlorophyll concentration value across the plurality of pixels in the second image is reduced; and determining an estimated chlorophyll concentration value for the plant based at least on the transmissive chlorophyll concentration value and the reflectance chlorophyll concentration value.

First claim

What is claimed is:

1. A method for determining chlorophyll content of a plant, comprising:
capturing a first image comprising light transmitted through a leaf of a plant;
capturing a second image comprising light reflected from the leaf of the plant;
estimating, from a plurality of pixels in the first image, a transmissive chlorophyll concentration value of the leaf;
determining, based on a plurality of pixels in the second image, bidirectional reflectance parameters for the second image;
determining, for the plurality of pixels in the second image, a plurality of reflectance chlorophyll concentration values, each reflectance chlorophyll concentration value being determined based on a respective one of the bidirectional reflectance parameters;
adjusting, based on a variance between the plurality of reflectance chlorophyll concentration values, at least one of the bidirectional reflectance parameters to reduce the variance between the plurality of reflectance chlorophyll concentration values across the plurality of pixels in the second image;
estimating, based on the adjusted bidirectional reflectance parameters, the reflectance chlorophyll concentration value for the leaf; and
determining an estimated chlorophyll concentration value for the plant based at least on the transmissive chlorophyll concentration value and the reflectance chlorophyll concentration value.

2. The method of claim 1 , wherein the transmissive chlorophyll concentration value and the reflectance chlorophyll concentration value are determined using a leaf model parameter, further comprising:
estimating, from the transmissive chlorophyll concentration value and the reflectance chlorophyll concentration value, a revised leaf model parameter; and
determining a second transmissive chlorophyll concentration value and a second reflectance chlorophyll concentration value using the revised leaf model parameter.

3. The method of claim 2 , wherein the revised leaf model parameter is estimated based on a difference between the transmissive chlorophyll concentration value and the reflectance chlorophyll concentration value.

4. The method of claim 1 , wherein estimating, from the plurality of pixels in the first image, the transmissive chlorophyll concentration value of the leaf comprises determining, from sensor spectral response characteristics of each pixel in the plurality of pixels in the first image, a triangular greenness index (TGI) for the pixel.

5. The method of claim 1 , further comprising:
capturing a third image comprising light passed through a first optical reference and a fourth image comprising light passed through a second optical reference, the first optical reference and second optical reference having transmissive characteristics corresponding to known transmissive chlorophyll levels; and
adjusting the transmissive chlorophyll concentration value of the leaf with reference to the known transmissive chlorophyll levels.

6. The method of claim 5 , wherein the first optical reference has transmissive characteristics corresponding to a known low transmissive chlorophyll level, and wherein the second optical reference has transmissive characteristics corresponding to a known high transmissive chlorophyll level.

7. The method of claim 1 , further comprising:
estimating, using a bidirectional reflectance parameter for each pixel in the plurality of pixels, a first reflectance chlorophyll concentration pixel value for each pixel in the plurality of pixels;
determining a first variance of the first reflectance chlorophyll concentration pixel value across the plurality of pixels;
modifying the bidirectional reflectance parameter for at least one pixel in the plurality of pixels;
estimating, using the modified bidirectional reflectance parameter for the at least one pixel, a second reflectance chlorophyll concentration pixel value for each pixel in the plurality of pixels;
determining a second variance of the second reflectance chlorophyll concentration pixel value across the plurality of pixels;
responsive to the first variance being less than the second variance, estimating the reflectance chlorophyll concentration value based on the first reflectance chlorophyll concentration pixel value of the leaf for each pixel in the plurality of pixels; and
responsive to the second variance being less than the first variance, estimating the reflectance chlorophyll concentration value based on the second reflectance chlorophyll concentration pixel value of the leaf for each pixel in the plurality of pixels.

8. The method of claim 7 , wherein modifying the bidirectional reflectance parameter for at least one pixel in the plurality of pixels comprises:
responsive to the at least one pixel in the plurality of pixels having a relatively high first reflectance chlorophyll concentration pixel value, adjusting the bidirectional reflectance parameter of the at least one pixel to be lower; and
responsive to the at least one pixel in the plurality of pixels having a relatively low first reflectance chlorophyll concentration pixel value, adjusting the bidirectional reflectance parameter of the at least one pixel to be higher.

9. The method of claim 7 , wherein estimating the reflectance chlorophyll concentration value based on the second reflectance chlorophyll concentration pixel value of the leaf for each pixel in the plurality of pixels comprises determining a mean of the second reflectance chlorophyll concentration pixel value of the leaf for the plurality of pixels.

10. The method of claim 9 , further comprising excluding, from the determination of the mean, pixels having a modified bidirectional reflectance parameter not within a defined deviation amount.

11. The method of claim 7 , wherein the bidirectional reflectance parameter is an initial bidirectional reflectance parameter, further comprising setting an initial bidirectional reflectance parameter for at least one pixel in the plurality of pixels, the initial bidirectional reflectance parameter determined by the sensor spectral response measurement of the at least one pixel.

12. The method of claim 11 , further comprising setting a first initial bidirectional reflectance parameter of 0.6 for at least one pixel having a highest sensor spectral response measurement in the plurality of pixels, and setting a second initial bidirectional reflectance parameter of 0.0 for at least one pixel having a lowest sensor spectral response measurement in the plurality of pixels.

13. The method of claim 1 , wherein the plant is a first plant further comprising:
estimating a second transmissive chlorophyll concentration value and a second reflectance chlorophyll concentration value for at least a second plant in a crop field including the first plant; and
generating a model of plant health for the crop field based at least on the transmissive chlorophyll concentration value, the reflectance chlorophyll concentration value, the second transmissive chlorophyll concentration value and the second reflectance chlorophyll concentration value.

14. The method of claim 13 , wherein the model of plant health for the crop field is a chlorophyll model indicating an estimated chlorophyll level of plants in a plurality of locations in the crop field.

15. The method of claim 1 , wherein the plant is a corn plant.

16. An image processing system comprising:
a memory;
an image receiving component; and
a processor configured to:
capture a first image comprising light transmitted through a leaf of a plant;
capture a second image comprising light reflected from the leaf of the plant;
estimate, fro

Assignees

• Raytheon Co

Inventors

• Young Darrell L
• Dekeyrel Charlotte
• Lewis Michael Henry

Classifications

• G01N21/4738

  Diffuse reflection (precedence is given to G01N21/55 - G01N21/57 if specular component is taken into consideration), e.g. also for testing fluids, fibrous materials · CPC title

• G01N21/359

  using near infrared light · CPC title

• G01N21/3563

  for analysing solids; Preparation of samples therefor · CPC title

• G01N33/0098 Primary

  Plants or trees (wood G01N33/46) · CPC title

• G01N21/31

  Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry {(G01N21/72 takes precedence)} · CPC title