Biosensor and production method for same

Patent metadata

Field	Value
Publication number	US-10619178-B2
Application number	US-201715689792-A
Country	US
Kind code	B2
Filing date	Aug 29, 2017
Priority date	Aug 30, 2016
Publication date	Apr 14, 2020
Grant date	Apr 14, 2020

Abstract

The disclosure relates to a biosensor including electrodes, a hydrophilic region or layer, and a reagent layer that contains an enzyme and a mediator, and methods of producing thereof.

First claim

What is claimed is:

1. A biosensor comprising:
an insulation substrate;
electrodes that are formed on the insulation substrate, the electrodes including a working electrode with two opposite ends and a counter electrode with two opposite ends, wherein said electrodes are disposed side by side in a first direction;
a continuous hydrophilic layer having higher hydrophilicity than a surrounding region thereof, comprising first ends, in a second direction orthogonal to the first direction, between said two opposite ends of the working electrode and between said two opposite ends of the counter electrode, and comprising second ends, in the first direction, disposed on a pair of working electrodes or a pair of counter electrodes; and
a reagent layer on the hydrophilic layer, the reagent layer comprising an enzyme and a mediator.

2. The biosensor according to claim 1 , wherein the reagent layer has a rectangular shape.

3. The biosensor according to claim 1 , wherein the distance between each of the pair of working electrodes or each of the pair of counter electrodes, and working electrodes or counter electrodes facing each of the pair of working electrodes or the pair of counter electrodes is the same.

4. The biosensor according to claim 1 , wherein the working electrode and the counter electrode are in parallel with each other.

5. The biosensor according to claim 1 , wherein not more than one continuous hydrophilic layer is formed on the working electrode or counter electrode.

6. The biosensor according to claim 1 , wherein
the working electrode has a neighboring region without an counter electrode, and/or
the working electrode or the counter electrode is only partially covered with the reagent layer.

7. The biosensor according to claim 1 , wherein the hydrophilic layer covers at least a part of the working electrode and at last two separate parts of the counter electrode.

8. The biosensor according to claim 1 , wherein a single continuous hydrophilic layer is formed on the electrodes.

9. The biosensor according to claim 1 , further comprising a spacer and a reinforcing material on the electrodes.

10. The biosensor according to claim 9 , wherein the hydrophilic layer is not in direct contact with the spacer.

11. A production method for biosensor, the method comprising:
forming, on an insulation substrate, electrodes including a working electrode with two opposite ends and a counter electrode with two opposite ends, wherein said electrodes are arranged side by side in a first direction;
forming a continuous hydrophilic layer on the electrodes, the hydrophilic layer (i) having higher hydrophilicity than a surrounding region thereof, (ii) comprising first ends, in a second direction orthogonal to the first direction, disposed between said two opposite ends of the working electrode and between said two opposite ends of the counter electrode, and (iii) comprising second ends, in the first direction, a pair of working electrodes or a pair of counter electrodes;
supplying, to the continuous hydrophilic layer, a reagent liquid comprising an enzyme and a mediator; and
solidifying the reagent liquid to form a reagent layer.

12. The production method according to claim 11 , wherein the reagent layer has a rectangular shape.

13. The production method according to claim 11 , wherein the distance between each of the pair of working electrodes or each of the pair of counter electrodes, and working electrodes or counter electrodes facing each of the pair of working electrodes or the pair of counter electrodes is the same.

14. The production method according to claim 11 , wherein the working electrode and the counter electrode are in parallel with each other.

15. The production method according to claim 11 , wherein not more than one continuous hydrophilic layer is formed on the working electrode or counter electrode.

16. The production method according to claim 11 , wherein
the working electrode has a neighboring region without an counter electrode, and/or
the working electrode or the counter electrode is only partially covered with the reagent layer.

17. The production method according to claim 11 , wherein the hydrophilic layer covers at least a part of the working electrode and at last two separate parts of the counter electrode.

18. The production method according to claim 11 , further comprising a spacer and a reinforcing material on the electrodes.

19. The production method according to claim 11 , wherein the hydrophilic layer is not in direct contact with the spacer.

Assignees

• Arkray Inc

Inventors

• Fukuda Kazuo

Classifications

• G01N27/26

  by investigating electrochemical variables; by using electrolysis or electrophoresis · CPC title

• G01N27/3272

  Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels (optical biosensors G01N33/52) · CPC title

• B01D61/00

  Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor (separation of gases or vapours by diffusion B01D53/22) · CPC title

• C12Q1/004 Primary

  mediator-assisted · CPC title

• B01D59/50

  Separation involving two or more processes covered by different groups selected from groups B01D59/02, B01D59/10, B01D59/20, B01D59/22, B01D59/28, B01D59/34, B01D59/36, B01D59/38, B01D59/44 · CPC title