Growth factor-free proliferation and differentiation of neural stem cells on inorganic extracellular nanomatrices

Patent metadata

Field	Value
Publication number	US-10619135-B2
Application number	US-201715600808-A
Country	US
Kind code	B2
Filing date	May 22, 2017
Priority date	May 23, 2016
Publication date	Apr 14, 2020
Grant date	Apr 14, 2020

Abstract

The present invention provides nanostructures for use in proliferation and differentiation of neural stem cells. The present invention also provides method of proliferating and differentiating neural stem cells.

First claim

The invention claimed is:

1. A nanostructure comprising a plurality of nanohelices or nanozigzags, wherein the plurality of nanohelices or nanozigzags are made of SiO 2 or TiO x , wherein x is in the range of 0.33≤x≤2,
wherein the nanohelices made of SiO 2 has a stiffness of 12.6±1.8 μN/nm;
wherein the nanozigzags made of SiO 2 has a stiffness of 19.7±2.3 μN/nm and an intermediate stiffness greatly favoring neuron commitment has an intermediate stiffness between 10-30 μN/nm;
wherein the plurality of nanohelices or nanozigzags made of TiO x has a shape independent stiffness of no more than 26 μN/nm.

2. The nanostructure according to claim 1 , wherein the nanostructure comprises a plurality of nanohelices, length of each of the nanohelix is at least 540 nm and each nanohelix comprises at least two pitches and having a pitch of at least 240 nm.

3. The nanostructure according to claim 1 , wherein the nanostructure comprises a plurality of nanozigzags, length of each of the nanozigzag is at least 550 nm and each nanozigzag comprises at least three pitches and having a pitch of at least 165 nm.

4. The nanostructure according to claim 1 , wherein the nanostructure comprises a plurality of left-handed oriented nanohelices.

5. The nanostructure according to claim 1 , wherein the nanostructure comprises a plurality of right-handed oriented nanohelices.

6. The nanostructure of claim 1 , wherein the nanostructure is manufactured by GLAD technique.

7. A method for inducing proliferation of stem cells comprises culturing the stem cells on the nanostructure of claim 1 in the absence of chemical growth factors.

8. The method according to claim 7 , wherein said stem cells are neural stem cells.

9. The method according to claim 8 , wherein the nanostructure comprises a plurality of nanohelices, length of each of nanohelix is at least 540 nm and each nanohelix comprises at least two pitches and having pitch of at least 240 nm.

10. The method according to claim 8 , wherein the nanostructure comprises a plurality of nanozigzags, length of each of nanozigzag is at least 550 nm and each nanozigzag comprises at least three pitches and having a pitch of at least 165 nm.

11. A method of inducing proliferation and differentiation of stem cells comprises culturing the stem cells on nanostructure of claim 1 , wherein the plurality of nanohelices or nanozigzags are made of SiO 2 .

12. The method of claim 11 , wherein the stem cells are neural stem cells.

Assignees

• Univ Hong Kong Baptist Univ

Inventors

• Yung Kin Lam
• Huang Zhifeng
• Lui Nga Ping

Classifications

• B82Y40/00

  Manufacture or treatment of nanostructures · CPC title

• Y10S977/766

  Bent wire, i.e. having nonliner longitudinal axis · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• B82Y30/00

  Nanotechnology for materials or surface science, e.g. nanocomposites · CPC title

• C01B33/18

  Preparation of finely divided silica neither in sol nor in gel form; After-treatment thereof (preparation of aerogels by dehydrating gels C01B33/158; treatment to enhance the pigmenting or filling properties C09C) · CPC title