Xylanases for solubilising arabinoxylan-containing material

Patent metadata

Field	Value
Publication number	US-10612014-B2
Application number	US-201715843023-A
Country	US
Kind code	B2
Filing date	Dec 15, 2017
Priority date	Aug 3, 2012
Publication date	Apr 7, 2020
Grant date	Apr 7, 2020

Abstract

The present invention relates to a method for solubilizing arabinoxylan-containing material (particularly insoluble arabinoxylan-containing material), comprising admixing a xylan-containing material with a xylanase comprising a polypeptide sequence shown herein as SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 1, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11 or SEQ ID No. 15, or a variant, homolog, fragment or derivative thereof having at least 75% identity with SEQ ID No. 3 or SEQ ID No. 2 or SEQ ID No. 1 or SEQ ID No. 9 or SEQ ID No. 10 or SEQ ID No. 11 or SEQ ID No. 15; or a polypeptide sequence which comprises SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 1, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11 or SEQ ID No. 15 with a conservative substitution of at least one of the amino acids; or a xylanase which is encoded by a nucleotide sequence shown herein as SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 17 or SEQ ID No. 18, or a nucleotide sequence which can hybridize to SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 17 or SEQ ID No. 18 under high stringency conditions, or a nucleotide sequence which has at least 75% identity with SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 17 or SEQ ID No. 18, or a nucleotide sequence which differs from SEQ ID No. 6 or SEQ ID No. 5 or SEQ ID No. 4 or SEQ ID No. 12 or SEQ ID No. 13 or SEQ ID No. 14 or SEQ ID No. 16 or SEQ ID No. 17 or SEQ ID No. 18 due to the degeneracy of the genetic code, or a xylanase obtainable (or obtained) from Fusarium verticilloides. The present invention also relates to a novel xylanase comprising (or consisting of) a polypeptide sequence shown herein as SEQ ID No. 3, SEQ ID No. 2 or SEQ ID No. 1, or a variant, homolog, fragment or derivative thereof having at least 99% identity with SEQ ID No. 3 or SEQ ID No. 2 or SEQ ID No. 1; or a xylanase which is encoded by a nucleotide sequence shown herein as SEQ ID No. 6, SEQ ID No. 5 or SEQ ID No. 4, or a nucleotide sequence which can hybridize to SEQ ID No. 4 or SEQ ID No. 5 under high stringency conditions, or a nucleotide sequence which has at least 97.7% identity (preferably 98% identity) with SEQ ID No. 6, SEQ ID No. 5 or SEQ ID No. 4. The present invention yet further relates to methods relating to feedstuffs, malting and brewing, processing of grain-based materials such as during the production of bioethanol or biochemical (e.g. bio-based isopropanol), or wheat gluten-starch separation processes and the like.

First claim

The invention claimed is:

1. A feed additive composition or a premix comprising a polypeptide i) having xylanase activity, ii) which is capable of degrading insoluble arabinoxylans, and iii) comprising the polypeptide sequence in SEQ ID NO:3, and optionally at least one mineral and/or at least one vitamin.

2. The feed additive composition or premix according to claim 1 which further comprises one or more of the enzymes selected from the group consisting of a protease, an amylase, and a phytase.

3. A feedstuff comprising a feed additive composition or premix according to claim 1 obtained by admixing a feed component with the feed additive composition or the premix of claim 1 .

4. A method for degrading xylan in xylan-containing material, by admixing said xylan-containing material with the feed additive composition or premix of claim 1 .

5. The method according to claim 4 wherein the xylan-containing material is selected from one or more of the group consisting of: a feed or feedstuff; a feed component; a grain-based material; a mash; a wort; a malt; malted barely; an adjunct, a barley mash; and a cereal flour.

6. The method according to claim 5 wherein the feed or feedstuff or feed component comprises or consists of corn, Distillers Dried Grains with Solubles (DDGS), corn Distillers Dried Grain Solubles (cDDGS), wheat, wheat bran or a combination thereof.

7. The method according to claim 6 wherein the feed or feedstuff is a corn-based feedstuff.

Assignees

• Dupont Nutrition Biosci Aps

Inventors

• Romero Millan Luis Fernando
• Lund Susan Arent
• Laursen Brian Soegaard
• Kiarie Elijah G
• Zhang Zhenghong
• Lau Rosalyn
• Yu Zheyong
• Kooy Floor K
• Van Tuijl Jan Hendrik A
• Koops Bart C
• Pedersen Mads Broegger
• Dalsgaard Soeren

Classifications

• C12P7/08

  produced as by-product or from waste or cellulosic material substrate · CPC title

• A23K50/30

  for swines · CPC title

• C12P7/10

  substrate containing cellulosic material · CPC title

• C12N9/2482 Primary

  Endo-1,4-beta-xylanase (3.2.1.8) · CPC title

• C13K1/06

  obtained by saccharification of starch or raw materials containing starch · CPC title