Thermophile-derived keratinase and use thereof

Patent metadata

Field	Value
Publication number	US-10612010-B2
Application number	US-201515509095-A
Country	US
Kind code	B2
Filing date	Sep 7, 2015
Priority date	Sep 5, 2014
Publication date	Apr 7, 2020
Grant date	Apr 7, 2020

Abstract

The keratinase according to the present invention rapidly and effectively decomposes hardly-decomposable keratin, and thus it is expected that the keratinase can be used for the effective treatment and the high value-added resource recovery of agricultural and livestock waste, which causes environmental problems (for example, a novel material for enzyme cosmetics), and can be used in an innovative enzymatic bioconversion technique utilizing various decomposition enzyme groups.

First claim

The invention claimed is:

1. A method for keratin decomposition comprising, a step of treating keratin with
i) at least one Thermotogales order derived enzyme or protein selected from the group consisting of:
an isolated cysteine desulfurase (CDS);
an isolated thermostable carboxypeptidase 1 (CBP);
an isolated thioredoxin-disulfide reductase (DSR); and
an isolated iron-sulfur assembly scaffold protein (SufE); and also
ii) a Thermotogales order-derived cell extract,
wherein (i) and (ii) are not the same.

2. The method for keratin decomposition of claim 1 , wherein the keratin is derived from poultry feathers.

3. The method for keratin decomposition of claim 1 , wherein the isolated cysteine desulfurase (CDS) consists of the amino acid sequence of SEQ ID NO: 1; the isolated thermostable carboxypeptidase 1 (CBP) consists of the amino acid sequence of SEQ ID NO: 3; the isolated thioredoxin-disulfide reductase (DSR); consists of the amino acid sequence of SEQ ID NO: 5; and the isolated iron-sulfur assembly scaffold protein (SufE) consists of the amino acid sequence of SEQ ID NO: 7.

4. The method for keratin decomposition of claim 1 ,
wherein the CDS, CBP, DSR, and SufE are encoded by a polynucleotide consisting of
the base sequence of SEQ ID NO: 2;
the base sequence of SEQ ID NO: 4;
the base sequence of SEQ ID NO: 6; and
the base sequence of SEQ ID NO: 8, respectively.

5. The method for keratin decomposition of claim 1 , wherein the Thermotogales order includes Caldotoga, Mesotoga, Thermopallium, Thermotoga, Fervidobacterium, Thermosipho, Kosmotoga, Thermococcoides, Marinitoga, Geotoga , or Petrotoga genus.

6. The method for keratin decomposition of claim 1 , further, comprising the step of treating the keratin with Dithiothreitol.

7. The method for keratin decomposition of claim 1 , wherein the Thermotogales order is Fervidobacterium islandicum AW-1 strain.

Assignees

• Kyungpook Nat Univ Ind Academic Coop Found

Inventors

• Lee Dong Woo
• Lee Yong Jik
• Jin Hyun Su
• Kim Ji Yeon
• Nam Gae Won
• Lee Sang Jae

Classifications

• C12N9/0051

  acting on a sulfur group of donors (1.8) · CPC title

• C12Y304/16005

  Carboxypeptidase C (3.4.16.5), i.e. carboxypeptidase Y · CPC title

• C12N9/00

  Enzymes; Proenzymes; Compositions thereof (preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; {enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113}); Processes for preparing, activating, inhibiting, separating or purifying enzymes (preparation of malt C12C1/00) · CPC title

• C12N15/52

  Genes encoding for enzymes or proenzymes · CPC title

• C12N9/13 Primary

  transferring sulfur containing groups (2.8) · CPC title