Polymerase Variants and Uses Thereof
US-2016333327-A1 · Nov 17, 2016 · US
Polymerase variants
US10612009B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10612009-B2 |
| Application number | US-201916398089-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 29, 2019 |
| Priority date | Feb 2, 2015 |
| Publication date | Apr 7, 2020 |
| Grant date | Apr 7, 2020 |
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- Title
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- Abstract
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Abstract
Official abstract text for this publication.
Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, I295, Y342, T343, I357, S360, L361, I363, S365Q, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, 1644, T647, I648, T651, I652, K655, W656, D657, V658, H660, F662, L690 and combinations thereof.
First claim
Opening claim text (preview).
What is claimed is: 1. An isolated polypeptide having a DNA polymerase activity comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 1, wherein the amino acid sequence having at least 95% sequence identity to SEQ ID NO: 1 comprises at least two amino acid substitutions relative to SEQ ID NO: 2, wherein one of said at least two amino acid substitutions corresponds to position T529 of SEQ ID NO: 2. 2. The isolated polypeptide of claim 1 , wherein the T529 substitution is a T529M/F substitution. 3. The isolated polypeptide of claim 1 , wherein said isolated polypeptide has a substitution corresponding to T529M. 4. The isolated polypeptide of claim 1 , wherein said at least two amino acid substitutions comprises a set of substitutions selected from the group consisting of: T529M+T647G+A547F; T529M+A610T+A547F; M641Y+T529M+A547F; N535L+N545K+T651Y+T529M; and N535I+N545K+T651Y+T529M. 5. The isolated polypeptide of claim 1 , wherein said polymerase comprises less than 10 mutations. 6. The isolated polypeptide of claim 1 , wherein at least one of the at least two corresponds to a position of SEQ ID NO: 2 selected from the group consisting of selected from the group consisting of Y225, M531, N535, G539, N545, A547, N552, A610, M641, Y629, T647, T651, I652, K655, D657, V658. 7. The isolated polypeptide of claim 1 , wherein at least one of the at least two substitutions is selected from the group consisting of selected from the group consisting of Y225L/T/I/F/A, M531H/Y/A/K/R/W/T/L/V, N535L/Y/M/K/I, G539Y/F, N545K/D/S/L/R, A547M/Y/W/F/V/S, N552L/M/S, A610T/E, M641L/Y, Y629 W/H/M, T647G/A/E/K/S, T651Y/F/M, I652Q/G/S/N/F/T, K655G/F/E/N, D657R/P/A, and V658L.
Assignees
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Classifications
DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase · CPC title
- C12N9/1252Primary
DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase · CPC title
containing a His-tag · CPC title
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Frequently asked questions
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- What does patent US10612009B2 cover?
- Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, I295, Y342, T343, I357, S360, L361, I363, S365Q, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, 1644, T647, I648, T651, I…
- Who is the assignee on this patent?
- Roche Molecular Systems Inc, Roche Sequencing Solutions Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/1252. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 07 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 5 related publications on this page (citations in our corpus or others sharing the same primary CPC).