Droplet forming devices and system with differential surface properties

Patent metadata

Field	Value
Publication number	US-10610865-B2
Application number	US-201815977824-A
Country	US
Kind code	B2
Filing date	May 11, 2018
Priority date	Aug 22, 2017
Publication date	Apr 7, 2020
Grant date	Apr 7, 2020

Abstract

Devices, systems, and their methods of use, for generating droplets are provided. One or more geometric parameters of a microfluidic channel can be selected to generate droplets of a desired and predictable droplet size.

First claim

What is claimed is:

1. A device for producing droplets of a first liquid in a second liquid, the device comprising:
a) a first channel having a first depth, a first width, a first proximal end, a first distal end, and a first surface comprising a material;
b) a shelf region having a second depth and a second width, wherein the second width is greater than the first width, wherein the shelf region comprises a hydrophobic coating different from the material of the first surface, and wherein the first distal end is in fluid communication with the shelf region; and
c) a collection reservoir configured to collect the droplets and comprising at least one wall that forms a step fluidically connected to the shelf region;

wherein the first liquid expands at the shelf region and step to produce droplets of the first liquid in the second liquid in the collection reservoir.

2. The device of claim 1 , further comprising a second channel having a second depth, a second width, a second proximal end, and a second distal end, wherein the second channel intersects the first channel between the first proximal and first distal ends.

3. The device of claim 2 , further comprising a second reservoir in fluid communication with the second proximal end.

4. The device of claim 1 , further comprising a first reservoir in fluid communication with the first proximal end.

5. A system for producing droplets of a first liquid in a second liquid, the system comprising:
a) a device for producing droplets, the device comprising:
i) a first channel having a first depth, a first width, a first proximal end, a first distal end, and a first surface comprising a material;
ii) a shelf region having a second depth and a second width, wherein the second width is greater than the first width, wherein the shelf region comprises a hydrophobic coating different from the material of the first surface, and wherein the first distal end is in fluid communication with the shelf region;
iii) a first reservoir in fluid communication with the first proximal end and comprising at least a portion of the first liquid; and
iv) a collection reservoir configured to collect the droplets and comprising at least one wall that forms a step fluidically connected to the shelf region; and

b) a second liquid contained in the shelf region and the collection reservoir, wherein the first liquid and the second liquid are immiscible,

wherein the first liquid expands at the shelf region and step to produce droplets of the first liquid in the second liquid in the collection reservoir.

6. The system of claim 5 , wherein the first reservoir further comprises particles.

7. The system of claim 5 , wherein the device further comprises a second channel having a second depth, a second width, a second proximal end, and a second distal end, wherein the second channel intersects the first channel between the first proximal and first distal ends.

8. The system of claim 7 , wherein the device further comprises a second reservoir in fluid communication with the second proximal end and comprises at least one portion of the first liquid.

9. The system of claim 7 , wherein the portion of the first liquid in the first channel and the portion of the first liquid in the second channel combine at the intersection of the first channel and second channel to form the first liquid.

10. The system of claim 7 , further comprising a controller operatively coupled to transport the portion of the first liquid in the first reservoir and the portion of first liquid in the second reservoir to the intersection.

11. The system of claim 5 , wherein the device further comprises a second channel having a second depth, a second width, a second proximal end, and a second distal end, where the second channel intersects the first channel between the first proximal and first distal ends; the second channel comprising a third liquid, the third liquid combining with the first liquid at the intersection; wherein the droplets comprise the third liquid.

12. The system of claim 11 , wherein the third liquid is aqueous or miscible with water.

Assignees

• 10X Genomics Inc

Inventors

• Bharadwaj Rajiv
• Makarewicz Anthony
• Lin Bill Kengli

Classifications

• G01N33/58

  involving labelled substances (G01N33/53 takes precedence) · CPC title

• G01N33/54313

  the carrier being characterised by its particulate form · CPC title

• B01L2300/0851

  Bottom walls · CPC title

• B01L2300/161

  Control and use of surface tension forces, e.g. hydrophobic, hydrophilic · CPC title

• B01L3/502784 Primary

  specially adapted for droplet or plug flow, e.g. digital microfluidics · CPC title