Combined organ and hematopoietic cells for transplantation tolerance of grafts

Patent metadata

Field	Value
Publication number	US-10603340-B2
Application number	US-201815947285-A
Country	US
Kind code	B2
Filing date	Apr 6, 2018
Priority date	Feb 26, 2013
Publication date	Mar 31, 2020
Grant date	Mar 31, 2020

Abstract

Methods and compositions are provided for combined transplantation of a solid organ and hematopoietic cells to a recipient, where tolerance to the graft is established through development of a persistent mixed chimerism. An individual with persistent mixed chimerism, usually for a period of at least six months, is able to withdraw from the use of immunosuppressive drugs after a period of time sufficient to establish tolerance.

First claim

What is claimed is:

1. A cellular product for establishing mixed chimerism in a solid organ transplant recipient, the product comprising:
greater than 1×10 5 CD34 + cells/kg recipient weight derived from a deceased donor; and
greater than 1×10 5 CD3 + cells/kg recipient weight derived from the deceased donor.

2. The cellular product of claim 1 , wherein the solid organ is kidney.

3. The cellular product of claim 1 , wherein the CD34 + cells and the CD3 + cells are HLA-matched to the solid organ transplant recipient.

4. The cellular product of claim 1 , wherein the CD34 + cells and the CD3 + cells are HLA-mismatched to the solid organ transplant recipient.

5. The cellular product of claim 1 , wherein the CD34 + cells and the CD3 + cells are provided in separate containers.

6. The cellular product of claim 1 , wherein the CD34 + cells and the CD3 + cells are provided as a mixture in a common container.

7. The cellular product of claim 1 , wherein the CD34 + cells and the CD3 + cells are from a bone marrow sample.

8. The cellular product of claim 1 , further comprising a cryopreservation medium.

9. The cellular product of claim 8 , wherein the cryopreservation medium comprises a polymeric carbohydrate.

10. The cellular product of claim 1 , wherein the CD34 + cells and the CD3 + cells are derived from the same donor.

Assignees

• Univ Leland Stanford Junior

Inventors

• Strober Samuel
• Lowsky Robert

Classifications

• A61P11/00

  Drugs for disorders of the respiratory system · CPC title

• A61K31/436

  the heterocyclic ring system containing a six-membered ring having oxygen as a ring hetero atom, e.g. rapamycin · CPC title

• A61K35/12 Primary

  Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells (vaccines or medicinal preparations containing antigens or antibodies A61K39/00) · CPC title

• A61K35/28

  Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells · CPC title

• A61P1/04

  for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants · CPC title