Detecting neoplasm

Patent metadata

Field	Value
Publication number	US-10590489-B2
Application number	US-201715724890-A
Country	US
Kind code	B2
Filing date	Oct 4, 2017
Priority date	Feb 15, 2008
Publication date	Mar 17, 2020
Grant date	Mar 17, 2020

Abstract

This document relates to methods and materials for detecting premalignant and malignant neoplasms. For example, methods and materials for determining whether or not a stool sample from a mammal contains nucleic acid markers or polypeptide markers of a neoplasm are provided.

First claim

What is claimed is:

1. A method, comprising:
(a) extracting genomic DNA from a biological sample of a human individual suspected of having precancer or colorectal cancer, wherein the biological sample is a stool sample;
(b) amplifying a portion of the extracted genomic DNA with primers specific for K-ras, and
measuring a K-ras mutation score by performing a digital melt curve analysis or quantitative allele-specific PCR; and

(c) treating a portion of the extracted genomic DNA with bisulfite;
amplifying the bisulfite-treated genomic DNA with primers specific for BMP3; and
measuring the methylation level of one or more CpG sites in BMP3 by methylation-specific PCR, quantitative methylation-specific PCR, methylation-sensitive DNA restriction enzyme analysis, quantitative bisulfite pyrosequencing, or bisulfite genomic sequencing PCR.

2. The method of claim 1 , wherein the primers specific for K-ras are selected from SEQ ID NOs: 2, 3, 4, 5, 6, 7 and 8.

Assignees

• Mayo Found Medical Education & Res

Inventors

• Taylor William R
• Harrington Jonathan J
• Quint Patrick S
• Zou Hongzhi
• Bergen Iii Harold R
• Smith David I
• Ahlquist David A

Classifications

• C12Y302/01001

  Alpha-amylase (3.2.1.1) · CPC title

• C12Q2600/154

  Methylation markers · CPC title

• G01N2560/00

  Chemical aspects of mass spectrometric analysis of biological material · CPC title

• C12Q1/37

  involving peptidase or proteinase · CPC title

• C12Q1/40

  involving amylase · CPC title