Microorganism for simultaneously producing L-amino acid and riboflavin, and method for producing L-amino acid and riboflavin using same

Patent metadata

Field	Value
Publication number	US-10590446-B2
Application number	US-201214362036-A
Country	US
Kind code	B2
Filing date	Oct 17, 2012
Priority date	Dec 1, 2011
Publication date	Mar 17, 2020
Grant date	Mar 17, 2020

Abstract

The present invention relates to a method for producing highly-concentrated L-amino acid and riboflavin simultaneously, and a microorganism for simultaneously producing L-amino acid and riboflavin. Specifically, the present invention relates to a modified microorganism for producing L-lysine or L-threonine, and riboflavin simultaneously, wherein the microorganism belonging to Corynebacterium sp. capable of producing L-lysine or L-threonine is modified by enhancing the activity of an enzyme family expressed by a rib operon which contains riboflavin biosynthesis gene family. Also, the present invention relates to a method for the simultaneous production of L-lysine or L-threonine, and riboflavin using the modified microorganism, and relates to a formulation or granular formulation, feed, and feed additive, containing L-lysine or L-threonine, and riboflavin produced from a culture medium of the modified microorganism.

First claim

The invention claimed is:

1. A modified Corynebacterium glutamicum microorganism for simultaneously producing L-lysine or L-threonine, and riboflavin,
wherein said modified Corynebacterium glutamicum microorganism has enhanced expression of a riboflavin (rib) operon as compared to the expression of a rib operon in a corresponding wild-type Corynebacterium glutamicum microorganism, wherein the rib operon comprises genes encoding riboflavin biosynthesis enzymes, and wherein the expression of the rib operon in said modified Corynebacterium glutamicum microorganism is enhanced by increasing intracellular copy number of the genes of the rib operon, replacing the native promoter of the rib operon with a stronger promoter, and a combination thereof,
wherein said modified Corynebacterium glutamicum microorganism has increased L-lysine or L-threonine-producing ability as compared to a corresponding wild-type Corynebacterium glutamicum microorganism, and
wherein the concentration of riboflavin produced by said modified Corynebacterium glutamicum microorganism is increased by 56 to 73-fold as compared to the concentration of riboflavin produced by a corresponding wild-type Corynebacterium glutamicum microorganism under the same conditions, while maintaining the increased production of L-lysine or L-threonine as compared to a corresponding wild-type Corynebacterium glutamicum microorganism.

2. The microorganism according to claim 1 , wherein the expression of the rib operon in said modified Corynebacterium glutamicum microorganism is enhanced by replacing the native promoter of the rib operon with a stronger promoter.

3. The microorganism according to claim 2 , wherein the stronger promoter comprises the nucleotide sequence of SEQ ID NO: 5 or 6.

4. The microorganism according to claim 1 , wherein the modified Corynebacterium glutamicum microorganism is the microorganism deposited with Korean Culture Center of Microorganisms (KCCM) under accession No. KCCM11220P, KCCM11221P or KCCM11223P.

5. A method for producing L-lysine or L-threonine, and riboflavin, comprising:
a) culturing the modified Corynebacterium glutamicum microorganism of claim 1 in a culture medium; and
b) producing L-lysine or L-threonine, and riboflavin by a fermentation process.

6. The method according to claim 5 , which further comprises granulating the fermented culture medium comprising L-lysine or L-threonine, and riboflavin.

7. The method according to claim 5 , which further comprises removing a bacterial sludge from the fermented culture medium comprising L-lysine or L-threonine, and riboflavin.

Assignees

• Cj Cheiljedang Corp

Inventors

• Park Sang Hee
• Moon Jun Ok
• Lim Sang Jo
• Kwon Do Hyun
• Lee Kyung Han
• Sung Jin Suck
• Kim Hyun Joon

Classifications

• C12P17/12 Primary

  containing a six-membered hetero ring · CPC title

• C12P25/00

  Preparation of compounds containing alloxazine or isoalloxazine nucleus, e.g. riboflavin · CPC title

• C12P13/08 Primary

  Lysine; Diaminopimelic acid; Threonine; Valine · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• A23V2002/00

  Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title