Acinetobacter lysins

Patent metadata

Field	Value
Publication number	US-10590403-B2
Application number	US-201515321905-A
Country	US
Kind code	B2
Filing date	Jun 26, 2015
Priority date	Jun 26, 2014
Publication date	Mar 17, 2020
Grant date	Mar 17, 2020

Abstract

Acinetobacter lysin polypeptides and variants peptides with killing activity against gram negative bacteria. Methods for treating bacterial infections or bacterial colonization using Acinetobacter lysin polypeptides.

First claim

What is claimed is:

1. A polypeptide, wherein the polypeptide comprises an amino acid sequence having the SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:20, or SEQ ID NO:21, and the polypeptide is conjugated to an antimicrobial peptide having the amino acid sequence SQSRESQC (SEQ ID NO:44) to yield a conjugated polypeptide and the conjugated polypeptide has antibacterial activity.

2. The conjugated polypeptide according to claim 1 , wherein the C-terminus of the polypeptide is conjugated to the antimicrobial peptide.

3. The conjugated polypeptide according claim 1 , wherein the N-terminus of the polypeptide is conjugated to the antimicrobial peptide.

4. The conjugated polypeptide according to claim 1 , wherein the conjugated polypeptide has antibacterial activity against a gram negative bacterium.

5. The conjugated polypeptide according to claim 1 , wherein the C-terminus of the polypeptide is conjugated to the antimicrobial peptide or the N-terminus of the polypeptide is conjugated to the antimicrobial peptide.

6. The conjugated polypeptide according to claim 5 , wherein the conjugated polypeptide has antibacterial activity against a gram negative bacterium.

7. A polypeptide, wherein the polypeptide comprises an amino acid sequence having the SEQ ID NO:1 or SEQ ID NO:21, or a fragment of the polypeptide consisting of the amino acid sequence of SEQ ID NO:43 (P307) and the polypeptide or polypeptide fragment is conjugated to an antimicrobial peptide having the amino acid sequence SQSRESQC (SEQ ID NO:44) to yield a conjugated polypeptide and the conjugated polypeptide has antibacterial activity.

8. The conjugated polypeptide according to claim 7 , wherein the C-terminus of the conjugated polypeptide is conjugated to the antimicrobial peptide.

9. The conjugated polypeptide according claim 7 , wherein the N-terminus of the conjugated polypeptide is conjugated to the antimicrobial peptide.

10. The conjugated polypeptide according to claim 7 , wherein the conjugated polypeptide has antibacterial activity against a gram negative bacterium.

11. The conjugated polypeptide according to claim 7 , wherein the C-terminus of the polypeptide or fragment is conjugated to the antimicrobial peptide or the N-terminus of the polypeptide or fragment is conjugated to the antimicrobial peptide.

12. The conjugated polypeptide according to claim 7 , wherein the conjugated polypeptide has antibacterial activity against a gram negative bacterium.

13. The polypeptide fragment of claim 7 , wherein the conjugated polypeptide has the amino acid sequence of SEQ ID NO:45.

Assignees

• Univ Rockefeller

Inventors

• Fischetti Vincent
• Schuch Raymond
• Lood Rolf
• Winer Benjamin

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P31/04

  Antibacterial agents · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• C07K2319/00

  Fusion polypeptide · CPC title

• C12N9/2402 Primary

  hydrolysing O- and S- glycosyl compounds (3.2.1) · CPC title