Medium composition and method for producing red blood cells using same
US-2016060601-A1 · Mar 3, 2016 · US
Culture medium composition and method of culturing cell or tissue using thereof
US10590380B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10590380-B2 |
| Application number | US-201715448557-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 2, 2017 |
| Priority date | Jul 24, 2012 |
| Publication date | Mar 17, 2020 |
| Grant date | Mar 17, 2020 |
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- Title
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Abstract
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The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition wherein indeterminate structures are formed in a liquid medium, the structures are uniformly dispersed in the solution and substantially retain the cells and/or tissues without substantially increasing the viscosity of the solution, thus affording an effect of preventing sedimentation thereof, and the like.
First claim
Opening claim text (preview).
The invention claimed is: 1. A medium composition comprising (i) a culture medium, (ii) deacylated gellan gum or a salt thereof, (iii) methylcellulose or a salt thereof, (iv) a recombinant fibroblast growth factor-2, and (v) a calcium ion. 2. The medium composition according to claim 1 , wherein the composition has a viscosity of not more than 8 mPa·s at 37° C. 3. The medium composition according to claim 2 , wherein the composition comprises deacylated gellan gum. 4. The medium composition according to claim 2 , wherein the composition comprises a salt of deacylated gellan gum. 5. The medium composition according to claim 2 , wherein the composition comprises methylcellulose. 6. The medium composition according to claim 2 , wherein the composition comprises a salt of methylcellulose. 7. The medium composition according to claim 2 , wherein the deacylated gellan gum or salt thereof and the methylcellulose or a salt thereof are present at a concentration which allows cells or a tissue to be cultured in suspension standing culture. 8. The medium composition according to claim 1 , wherein the composition comprises deacylated gellan gum. 9. The medium composition according to claim 1 , wherein the composition comprises a salt of deacylated gellan gum. 10. The medium composition according to claim 1 , wherein the composition comprises methylcellulose. 11. The medium composition according to claim 1 , wherein the composition comprises a salt of methylcellulose. 12. The medium composition according to claim 1 , wherein the deacylated gellan gum or salt thereof and the methylcellulose or a salt thereof are present at a concentration which allows cells or a tissue to be cultured in suspension standing culture.
Assignees
Inventors
Classifications
Microcarriers · CPC title
involving specific cell types · CPC title
Liver cells · CPC title
Tumour cells; Cancer cells · CPC title
Calcium; Ca chelators; Calcitonin · CPC title
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- What does patent US10590380B2 cover?
- The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition wherein indeterminate structures are formed in a liquid medium, the structures are uniformly dispersed in the solution and substantially retain the cells and/or tissues without substantially increasing the viscosity of the solution, …
- Who is the assignee on this patent?
- Nissan Chemical Ind Ltd, Univ Kyoto
- What technology area does this patent fall under?
- Primary CPC classification G01N33/5011. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Mar 17 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).