Multi-stage purification method and apparatus of extracellular vesicles using aqueous solution two-phase system

Patent metadata

Field	Value
Publication number	US-10590372-B2
Application number	US-201615560561-A
Country	US
Kind code	B2
Filing date	Mar 3, 2016
Priority date	Mar 24, 2015
Publication date	Mar 17, 2020
Grant date	Mar 17, 2020

Abstract

This disclosure relates to a multi-stage method and apparatus for purifying extracellular vesicles using an aqueous two-phase system, in which extracellular vesicles mixed and contaminated with proteins can be isolated and purified in a large amount at high purity within a short time through a multi-stage purification process using an aqueous two-phase system, thereby removing 95% or more of proteins and obtaining high-purity extracellular vesicles, resulting in very high processing efficiency compared to conventional techniques. In particular, the method of the disclosure does not require an expensive device or material such as an ultracentrifuge or an antibody, and can be performed at low cost and is thus economical and highly competitive. Furthermore, the extracellular vesicles thus isolated and purified can be employed in analysis methods such as RT-PCR or western blot, and can be utilized for research fields and disease diagnosis using the same.

First claim

The invention claimed is:

1. A multi-stage purification method of extracellular vesicles using an aqueous two-phase system, comprising the steps of:
(a) preparing a mixture by mixing a first material and a second material with (1) a body fluid containing extracellular vesicles and proteins, or (2) an aqueous solution containing extracellular vesicles mixed and contaminated with proteins;
(b) separating the mixture to obtain an aqueous two phase system having an upper solution and a lower solution without ultracentrifugation;
(c) removing the upper solution including the first material from the aqueous two-phase system;
(d) supplying and mixing the lower solution with a new upper solution including a new quantity of the first material; and
(e) performing the steps (b) to (d) at least two times and then recovering the extracellular vesicles from the lower solution,
wherein the body fluid comprises at least one fluid selected from the group consisting of whole blood, serum, peritoneal fluid, breast milk, and urine,
wherein the first material and the second material are immiscible with each other,
wherein the first material is selected from the group consisting of polyethylene glycol, polyvinyl pyrrolidone, polyvinyl alcohol, and copolymers of sucrose and epichlorohydrin combinations thereof, and
the second material is selected from the group consisting of EOPO (ethylene oxide propylene oxide), dextran, levan, poly(vinyl methyl ethyl ether), ammonium sulfate, sodium sulfate, magnesium sulfate, potassium phosphate, and sodium carbonate combinations thereof.

2. The multi-stage purification method of claim 1 , wherein the second material is EOPO.

3. The multi-stage purification method of claim 1 , wherein the first material is polyethylene glycol and the second material is selected from the group consisting of dextran, levan, poly(vinyl methyl ethyl ether), ammonium sulfate, sodium sulfate, magnesium sulfate, potassium phosphate, and sodium carbonate.

4. The multi-stage purification method of claim 1 , wherein the first material is polyethylene glycol and the second material is dextran.

5. The multi-stage purification method of claim 1 , further comprising controlling an attractive force or a repulsive force of molecules in the aqueous two-phase system by adding an additive to the aqueous two-phase system, after the step (a) and before the step (b).

6. The multi-stage purification method of claim 1 , wherein the aqueous two phase system obtained in the step (b) is further subject to centrifugation at 500˜2,000×g-force.

7. The multi-stage purification method of claim 1 , wherein the step (b) further comprises applying ultrasonic waves or supplying microbubbles to the aqueous two-phase system.

Assignees

• Postech Acad Ind Found

Inventors

• Park Jae Sung
• Shin Hyun Woo

Classifications

• G01N33/6803

  General methods of protein analysis not limited to specific proteins or families of proteins · CPC title

• C12M1/264 Primary

  Devices involving centrifugal, centripetal or rotational forces · CPC title

• B01D11/0488

  Flow sheets · CPC title

• B01D17/0217

  by centrifugal force · CPC title

• C12M1/26

  Inoculator or sampler · CPC title