Two pass macro image

Patent metadata

Field	Value
Publication number	US-10585273-B2
Application number	US-201816146991-A
Country	US
Kind code	B2
Filing date	Sep 28, 2018
Priority date	Sep 29, 2017
Publication date	Mar 10, 2020
Grant date	Mar 10, 2020

Abstract

Two-pass capture of a macro image. In an embodiment, a scanning apparatus comprises a stage, a high-resolution camera, and a lens that provides a field of view, substantially equal in width to a slide width, to the high-resolution camera. The apparatus also comprises a first illumination system for transmission-mode illumination, and a second illumination system for reflection-mode illumination. Processor(s) move the stage in a first direction to capture a first macro image of a specimen during a single pass while the field of view is illuminated by the first illumination system, and move the stage in a second direction to capture a second macro image of the specimen during a single pass while the field of view is illuminated by the second illumination system. The processor(s) identify artifacts in the second macro image, and, based on those artifacts, correct the first macro image to generate a modified first macro image.

First claim

What is claimed is:

1. A digital slide scanning apparatus comprising:
a stage configured to support a glass slide;
a high-resolution camera;
a first lens defining a macro image optical path to the high-resolution camera, wherein the first lens is configured to provide a field of view to the high-resolution camera, and wherein the field of view has a width that is substantially equal to a complete width of a glass slide;
a first illumination system optically coupled with the first lens and configured for transmission-mode illumination of the field of view from below a glass slide on the stage;
a second illumination system optically coupled with the first lens and configured for reflection-mode illumination of the field of view from above a glass slide on the stage, wherein the second illumination system is configured to angle illumination light in a pattern such that substantially none of its illumination light is reflected along the optical path to the high-resolution camera; and
at least one processor configured to, while a stage is supporting a glass slide,
while the field of view is illuminated by the first illumination system, capture a first macro image of a specimen on the glass slide using the high-resolution camera while the glass slide is under the first lens,
while the field of view is illuminated by the second illumination system, capture a second macro image of the specimen on the glass slide using the high-resolution camera while the glass slide is under the first lens,
identify image artifacts in the second macro image, and,
based on the identified image artifacts in the second macro image, correct the first macro image to generate a modified first macro image.

2. The digital slide scanning apparatus of claim 1 , wherein the at least one processor is further configured to use the modified first macro image to find tissue in the specimen and select an initial focus point for scanning the specimen.

3. The digital slide scanning apparatus of claim 1 , wherein the first illumination system further comprises a diffuser.

4. The digital slide scanning apparatus of claim 1 , wherein the first macro image is captured before the second macro image.

5. The digital slide scanning apparatus of claim 1 , wherein the first macro image is captured after the second macro image.

6. The digital slide scanning apparatus of claim 1 , further comprising a second lens for capturing high-resolution images at high magnification, wherein the second lens defines another optical path to the high-resolution image that is different than the macro image optical path, and wherein the second lens is configured to provide a smaller field of view than the first lens with a width that is less than a complete width of a glass slide.

7. The digital slide scanning apparatus of claim 1 , wherein identifying image artifacts in the second macro image comprises generating an artifact mask from the second macro image.

8. The digital slide scanning apparatus of claim 7 , wherein correcting the first macro image comprises applying the artifact mask to the first macro image.

9. The digital slide scanning apparatus of claim 8 , wherein correcting the first macro image comprises, prior to applying the artifact mask to the first macro image, pre-processing the first macro image to apply one or more of illumination correction, background offsetting, or background noise removal to the first macro image.

10. The digital slide scanning apparatus of claim 7 , wherein identifying artifacts in the second macro image comprises, prior to generating the artifact mask, pre-processing the second macro image to apply background offsetting to the second macro image.

11. The digital slide scanning apparatus of claim 1 , wherein the at least one processor is further configured to, based on the modified first macro image, determine an area of the specimen to scan.

12. The digital slide scanning apparatus of claim 1 , wherein correcting the first macro image comprises altering intensity levels in pixels, representing artifacts, in the first macro image, according to intensity levels in pixels surrounding those pixels that represent artifacts.

13. The digital slide scanning apparatus of claim 1 ,
wherein capturing the first macro image comprises moving the stage in a first direction to capture the first macro image of the specimen on the glass slide using the high-resolution camera during a single pass of the glass slide under the first lens, and
wherein capturing the second macro image comprises moving the stage in a second direction to capture the second macro image of the specimen on the glass slide using the high-resolution camera during a single pass of the glass slide under the first lens.

14. The digital slide scanning apparatus of claim 13 , wherein the second direction is opposite the first direction.

15. A method for generating a macro image in a digital slide scanning apparatus that comprises a stage configured to support a glass slide, a high-resolution camera, a lens defining a macro image optical path to the high-resolution camera and configured to provide a field of view to the high-resolution camera that has a width that is substantially equal to a complete width of a glass slide, a first illumination system optically coupled with the first lens and configured for transmission-mode illumination of the field of view from below a glass slide on the stage, a second illumination system optically coupled with the first lens and configured for reflection-mode illumination of the field of view from above a glass slide on the stage, and at least one processor, the method comprising, by the at least one processor of the digital slide scanning apparatus:
while the field of view is illuminated by the first illumination system, capturing a first macro image of a specimen on the glass slide using the high-resolution camera while the glass slide is under the first lens;
while the field of view is illuminated by the second illumination system, capturing a second macro image of the specimen on the glass slide using the high-resolution camera while the glass slide is under the first lens;
identifying image artifacts in the second macro image; and,
based on the identified image artifacts in the second macro image, correcting the first macro image to generate a modified first macro image.

Assignees

• Leica Biosystems Imaging Inc

Inventors

• Chen Leng-Chun
• Zou Yunlu
• Olson Allen
• Najmabadi Peyman
• Crandall Greg
• Newberg Nicholas
• Stearrett Aaron

Classifications

• G02B21/0072

  details concerning resolution or correction, including general design of CSOM objectives · CPC title

• G02B21/0032

  Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers (G02B21/0036 - G02B21/008; means for illumination of specimens in general G02B21/06) · CPC title

• G02B21/008 Primary

  Details of detection or image processing, including general computer control · CPC title

• H04N5/3692

  Electricity · mapped topic

• H04N5/217

  Electricity · mapped topic