Digital assay for quantifying and concentrating analytes

Patent metadata

Field	Value
Publication number	US-10585091-B2
Application number	US-201615000864-A
Country	US
Kind code	B2
Filing date	Jan 19, 2016
Priority date	Jul 19, 2013
Publication date	Mar 10, 2020
Grant date	Mar 10, 2020

Abstract

Provided herein are methods, compositions, and devices for the identification and quantification of analytes, such as nucleic acids, proteins, cells or other biological samples. The methods, compositions, and devices are suited for accurate, portable analysis of small amounts of analyte.

First claim

What is claimed is:

1. A method for analyzing a fluid sample, comprising:
providing a device comprising
a fluid inlet,
a fluid receiving structure,
a fluid path connecting the fluid inlet and the receiving structure,
a plurality of digital units arranged in capture regions located in the fluid path, each digital unit capable of capturing at least one analyte comprising nucleic acid, wherein the capture regions comprises
a first capture region comprising a first digital unit, and
a second capture region opposed to the first capture region, wherein the second capture region comprises a second digital unit, and wherein the first capture region or the second capture region is configured to slip relative to the other;

introducing a fluid sample into the device via the fluid inlet, the fluid sample comprising analytes;
flowing the fluid sample in the fluid path from the fluid inlet to the fluid receiving structure;
depleting analytes from the fluid sample by capturing analytes with the digital units as it flows through the array of capture regions;
slipping the first capture region or the second capture region relative to the other to isolate the first digital unit from the second digital unit;
performing digital amplification of the analytes in the isolated digital units, wherein the digital amplification generates a signal in response to presence of the at least one analyte; and
detecting the presence or absence of a signal generated from each of the digital units to determine the concentration or identity of the analytes in the fluid sample.

2. The method of claim 1 , wherein the slipping comprises actuating a portion of the device.

3. The method of claim 1 , wherein the determining comprises identifying the number of positive signals or negative signals generated from said digital units isolated from said fluid path.

4. The method of claim 1 , wherein said first digital unit generates a positive signal, and wherein said second digital unit does not generate a positive signal, and wherein a relative location of said first and second digital units along said fluid path before isolation is used to determine said concentration.

5. The method of claim 1 , wherein the flowing comprises flowing the fluid sample through the digital units.

6. The method of claim 1 , wherein the digital amplification comprises polymerase chain reaction (PCR).

7. The method of claim 1 , wherein the digital amplification comprises isothermal amplification.

8. The method of claim 3 , comprising classifying a digital unit as positive based on presence of detected signal in the digital unit.

9. The method of claim 3 , comprising classifying a digital unit as negative based on absence of detected signal in the digital unit.

10. The method of claim 3 , comprising generating a background signal in a negative control digital unit.

11. The method of claim 10 , comprising classifying a digital unit as positive if the detected signal in the digital unit exceeds the background signal.

12. The method of claim 10 , comprising classifying a digital unit as negative if the generated signal in the digital unit does not exceed the background signal.

13. A method for analyzing a sample, comprising:
providing a device comprising
a fluid inlet,
a fluid receiving structure,
a fluid path connecting the fluid inlet and the receiving structure,
a plurality of digital units located in the fluid path, each digital unit capable of capturing at least one analyte comprising nucleic acid, wherein the plurality of digital units comprise, in the direction from the fluid inlet towards the fluid receiving structure,
a first digital unit, and
a second digital unit;

introducing a fluid sample into the device via the fluid inlet, the fluid sample comprising analytes;
flowing a fluid sample in the fluid path from the fluid inlet to the fluid receiving structure;
depleting analytes from the fluid sample by capturing analytes with the digital units as it flows from the fluid inlet to the fluid receiving structure;
isolating the digital units;
performing digital amplification of the analytes in the isolated digital units, wherein the digital amplification generates a signal in response to presence of the at least one analyte; and
detecting the presence or absence of a signal generated from each of the digital units to determine the concentration or identity of the analytes in the fluid sample.

14. The method of claim 13 , wherein the isolating comprises actuating a portion of the device.

15. The method of claim 13 , wherein the determining comprises generating a profile of each digital unit in the flow path, wherein the profile comprises
a positive or negative classification of the digital unit based on presence or absence detected signal in the digital unit, and
location of the digital unit in the fluid path.

16. The method of claim 13 , wherein the determining comprises counting a number of the digital units along the fluid path up to a location in the fluid path where one or more of the digital units exhibit an absence of the signal.

17. The method of claim 13 , wherein the flowing comprises flowing the fluid sample through the digital units.

18. The method of claim 13 , wherein the digital amplification comprises polymerase chain reaction (PCR).

19. The method of claim 13 , wherein the digital amplification comprises isothermal amplification.

20. The method of claim 15 , comprising classifying a digital unit as positive based on presence of detected signal in the digital unit.

21. The method of claim 15 , comprising classifying a digital unit as negative based on absence of detected signal in the digital unit.

22. The method of claim 13 , wherein a total number of binding sites contained within the fluid path, the binding sites capable of binding the at least one analyte, exceeds a total number of analytes in the fluid sample.

23. The method of claim 1 , wherein a total number of binding sites contained within the fluid path, the binding sites capable of binding the at least one analyte, exceeds a total number of analytes in the fluid sample.

Assignees

• California Inst Of Techn
• Univ Chicago

Inventors

• Ismagilov Rustem F
• Mccalla Stephanie
• Schlappi Travis
• Huynh Toan
• Rolando Justin
• Liu Weishan
• Ge Shencheng
• Kreutz Jason E

Classifications

• B01L2200/0668

  Trapping microscopic beads · CPC title

• B01L2300/0819

  Microarrays; Biochips · CPC title

• B01L3/502761

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title

• B01L2400/0644

  rotary valves · CPC title