Methods, carrier assemblies, and systems for imaging samples for biological or chemical analysis

Patent metadata

Field	Value
Publication number	US-10584374-B2
Application number	US-201815967555-A
Country	US
Kind code	B2
Filing date	Apr 30, 2018
Priority date	Mar 24, 2015
Publication date	Mar 10, 2020
Grant date	Mar 10, 2020

Abstract

Method includes positioning a first carrier assembly on a system stage. The carrier assembly includes a support frame having an inner frame edge that defines a window of the support frame. The first carrier assembly includes a first substrate that is positioned within the window and surrounded by the inner frame edge. The first substrate has a sample thereon. The method includes detecting optical signals from the sample of the first substrate. The method also includes replacing the first carrier assembly on the system stage with a second carrier assembly on the system stage. The second carrier assembly includes the support frame and an adapter plate held by the support frame. The second carrier assembly has a second substrate held by the adapter plate that has a sample thereon. The method also includes detecting optical signals from the sample of the second substrate.

First claim

What is claimed is:

1. A carrier assembly comprising:
a support frame having an inner frame edge that defines a window of the support frame;
an adapter plate coupled to the support frame and positioned within the window, the adapter plate including a plate body having an inner plate edge that defines a pocket for receiving a substrate that is sized smaller than the window, the inner plate edge also defining a holding recess that opens to the pocket; and
a movable datum block positioned within the holding recess, the datum block being movable between a retracted position and an engaged position, the datum block configured to engage the substrate when the datum block is in the engaged position and press the substrate against an opposing surface of the adapter plate to hold the substrate within the pocket.

2. The carrier assembly of claim 1 , wherein the adapter plate extends parallel to an XY plane and faces in a direction along a Z axis, the datum block providing a holding force that is directed along the XY plane.

3. The carrier assembly of claim 1 , wherein the pocket at least one of coincides with a receiving plane that intersects the inner frame edge or is positioned below the receiving plane.

4. The carrier assembly of claim 1 , wherein the adapter plate is slidable along an XY plane within the window.

5. The carrier assembly of claim 1 , wherein the datum block comprises an engagement surface that extends into the pocket and is to engage the substrate.

6. The carrier assembly of claim 1 , further comprising: a passage at least partially defined between an edge of the adapter plate and a portion of an edge of the substrate after the substrate is received, wherein the passage is sized and shaped to permit a portion of an individual's digit to be inserted therein and engage the substrate edge for loading or removing the substrate.

7. The carrier assembly of claim 1 , wherein the support frame comprises a frame body comprising a plurality of apertures sized and shaped to receive respective datums of a system stage.

8. The carrier assembly of claim 7 , wherein one or more of the datums of the system stage is to stop movement of the adapter plate.

9. The carrier assembly of claim 8 , wherein one or more of the datums of the system is to stop movement of a second substrate positioned in the window when the adapter plate is removed from the carrier assembly.

10. The carrier assembly of claim 1 , wherein the plate body of the adapter plate comprises a substrate platform within the pocket to have the substrate positioned thereon.

11. The carrier assembly of claim 10 , wherein the plate body of the adapter plate comprises a recess that surrounds the substrate platform.

12. A carrier assembly comprising:
a support frame having an inner frame edge that defines a window of the support frame; and
an adapter plate removably coupled to the support frame and positioned within the window, the inner frame edge defining a substrate-receiving recess positioned above the adapter plate, the substrate-receiving recess being configured to receive a first planar substrate, the adapter plate including a plate body having an inner plate edge that defines a pocket for receiving a second planar substrate that is sized smaller than the first planar substrate, the pocket existing at least partially below the substrate-receiving recess.

13. The carrier assembly of claim 1 , wherein the adapter plate is removably coupled to the support frame.

14. The carrier assembly of claim 13 , wherein when the adapter plate is removed, the window is to receive a second substrate larger than the substrate received in the pocket.

15. The carrier assembly of claim 14 , wherein the inner frame edge is to engage an edge of the second substrate.

16. The carrier assembly of claim 14 , wherein the substrate received in the pocket comprises a microarray comprising a plurality of array sections, each of the array sections comprising a plurality of stripes.

17. The carrier assembly of claim 16 , wherein the second substrate is a flow cell.

18. The carrier assembly of claim 12 , wherein the first planar substrate comprises a microarray and wherein the second planar substrate comprises a flow cell.

19. The carrier assembly of claim 12 , wherein the inner frame edge defines apertures that are sized and shape to receive datums for aligning the second planar substrate.

20. A carrier assembly system, comprising:
a carrier assembly comprising:
a support frame having an inner frame edge that defines a window of the support frame;
an adapter plate configured to be removably coupled to the support frame and positioned within the window, the adapter plate comprising a plate body having an inner plate edge that defines a pocket for receiving a first substrate that is sized smaller than the window;

the first substrate; and
a second substrate that is sized larger than the first substrate, wherein the window is configured to receive the second substrate when the adapter plate is not coupled to the support frame.

Assignees

• Illumina Cambridge Ltd

Inventors

• Rawlings Stephen
• Rao Venkatesh Mysore Nagaraja
• Ang Beng Keong
• Udpa Nitin

Classifications

• G01N2035/0091

  GUI [graphical user interfaces] · CPC title

• G01N35/04

  Details of the conveyor system {(G01N35/021 - G01N35/028 take precedence)} · CPC title

• G01N2035/00356

  Holding samples at elevated temperature (incubation) · CPC title

• G02B21/34

  Microscope slides, e.g. mounting specimens on microscope slides · CPC title

• B01L9/52

  Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips · CPC title