Method and carrier complexes for delivering molecules to cells

Patent metadata

Field	Value
Publication number	US-10584182-B2
Application number	US-201615067726-A
Country	US
Kind code	B2
Filing date	Mar 11, 2016
Priority date	May 1, 2003
Publication date	Mar 10, 2020
Grant date	Mar 10, 2020

Abstract

The invention relates to carrier complexes and methods for delivering molecules to cells. The carrier complexes comprises a molecule and an aromatic cationic peptide in accordance with the invention. In one embodiment, the method for delivering a molecule to a cell comprises contacting the cell with a carrier complex. In another embodiment, the method for delivering a molecule to a cell comprises contacting the cell with a molecule and an aromatic cationic peptide.

First claim

What is claimed is:

1. A carrier complex comprising a molecule conjugated to an aromatic cationic peptide, wherein the aromatic cationic peptide is selected from the group consisting of:
Phe-D-Arg-Phe-Lys-NH 2 (Phe 1 -DALDA),
D-Arg-2′,6′Dmt-Lys-Phe-NH 2 , and
2′,6′-Dmp-D-Arg-Phe-Lys-NH 2 (Dmp 1 -DALDA),
and wherein the molecule is a lipid, an enzyme, an antibody, or a neurotrophic growth factor.

2. The carrier complex according to claim 1 , wherein the molecule is conjugated to the aromatic cationic peptide by a linker.

3. The carrier complex according to claim 2 , wherein the linker is a component of the aromatic-cationic peptide.

4. The carrier complex according to claim 2 , wherein the linker is a component of the molecule.

5. The carrier complex according to claim 1 , wherein the molecule and aromatic cationic peptide are chemically bonded.

6. The carrier complex according to claim 1 , wherein the molecule and aromatic cationic peptide are physically bonded.

7. A method for delivering a molecule to a cell, the method comprising contacting the cell with a carrier complex, wherein the carrier complex comprises the molecule conjugated to an aromatic cationic peptide, wherein the aromatic cationic peptide is selected from the group consisting of:
Phe-D-Arg-Phe-Lys-NH 2 (Phe 1 -DALDA),
D-Arg-2′,6′-Dmt-Lys-Phe-NH 2 , and
2′,6′-Dmp-D-Arg-Phe-Lys-NH 2 (Dmp 1 -DALDA);
and wherein the molecule is a lipid, an enzyme, an antibody, or a neurotrophic growth factor.

8. The method according to claim 7 , wherein the molecule is conjugated to the aromatic cationic peptide by a linker.

9. The method according to claim 8 , wherein the linker is a component of the aromatic-cationic peptide.

10. The method according to claim 8 , wherein the linker is a component of the molecule.

11. The method according to claim 7 , wherein the molecule and aromatic cationic peptide are chemically bonded.

12. The method according to claim 7 , wherein the molecule and aromatic cationic peptide are physically bonded.

Assignees

• Cornell Res Foundation Inc

Inventors

• Szeto Hazel H
• Zhao Kesheng
• Robertson Hugh
• Birk Alex V

Classifications

• A61P39/06

  Free radical scavengers or antioxidants · CPC title

• A61P39/00

  General protective or antinoxious agents · CPC title

• A61P9/00

  Drugs for disorders of the cardiovascular system · CPC title

• A61P35/00

  Antineoplastic agents · CPC title

• A61P31/04

  Antibacterial agents · CPC title