Mixed mode ligands

Patent metadata

Field	Value
Publication number	US-10583429-B2
Application number	US-201514657701-A
Country	US
Kind code	B2
Filing date	Mar 13, 2015
Priority date	Mar 14, 2014
Publication date	Mar 10, 2020
Grant date	Mar 10, 2020

Abstract

The present disclosure is directed to mixed mode chromatography media comprising a ligand directly attached to a solid support. In some aspects, the ligand has a chemical formula of

The mixed mode chromatography media is useful for binding and purifying proteins from a solution.

First claim

What is claimed is:

1. A mixed-mode chromatography medium comprising a ligand coupled to a solid support, said ligand is:

wherein the curving line indicates where the ligand is directly coupled to the solid support without a spacer through an amine on the benzyl ring attached at the ortho position, wherein the solid support comprises a copolymer of 3-allyloxy-1,2-propanediol and vinyl pyrrolidinone; wherein the mixed-mode chromatography medium is in contact with a source solution maintained at a pH of 4.0 to 6.0 such that a target protein from the source solution binds to the ligand.

2. The mixed-mode chromatography medium of claim 1 , wherein said solid support has pores of a median diameter of 0.5 micron or greater with substantially no pores of 0.1 micron or less in diameter.

3. The mixed-mode chromatography medium of claim 1 , wherein said solid support is a membrane.

4. The composition of claim 1 , wherein the source solution is at pH 5.

5. The composition of claim 1 , wherein the target protein is an antibody, enzyme, clotting factor, transcription factor, growth regulator or phosphoprotein.

6. The composition of claim 1 , wherein the target protein is selected from the group consisting of lysozyme, papain, aprotinin, avidin, bovine trypsin, ribonuclease A, ribonuclease B, trypsinogen, α-chymotrypsin, ubiquitin, porcine trypsin, conalbumin, Horse Cytochrome C, α-chymotrypsinogen A, bovine serum albumin, human serum albumin, β-lactoglobulin B, β-lactoglobulin A, α-lactalbumin A and ovalbumin.

7. The chromatography composition of claim 1 , wherein the source solution comprises sodium chloride.

Assignees

• Bio Rad Laboratories
• Rensselaer Polytech Inst

Inventors

• Cramer Steven
• Woo James
• Chen Hong
• Liao Jiali
• Frost Russell

Classifications

• B01J39/26

  Cation exchangers for chromatographic processes · CPC title

• B01J20/28085

  being more than 50 nm, i.e. macropores · CPC title

• B01J2220/82

  Shaped bodies, e.g. monoliths, plugs, tubes, continuous beds · CPC title

• B01J20/286

  Phases chemically bonded to a substrate, e.g. to silica or to polymers · CPC title

• B01J20/3285

  Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic · CPC title