Systems and methods for relay ionization

Patent metadata

Field	Value
Publication number	US-10580634-B2
Application number	US-201916518291-A
Country	US
Kind code	B2
Filing date	Jul 22, 2019
Priority date	Mar 9, 2015
Publication date	Mar 3, 2020
Grant date	Mar 3, 2020

Abstract

The invention generally relates to systems and methods for relay ionization of a sample. In certain aspects, the invention provides systems that include an ion source that generates ions, a sample emitter configured to hold a sample, and a mass spectrometer. The system is configured such that the ions generated by the ion source are directed to interact with the sample emitter, thereby causing the sample to be discharged from the sample emitter and into the mass spectrometer.

First claim

What is claimed is:

1. A system for analyzing a sample, the system comprising:
an ion source that generates ions;
a plurality of sample emitters, each configured to hold a sample; and
a mass spectrometer;
wherein the system is configured such that at any one time, the ion source is aligned with one of the plurality of sample emitters and the ions generated by the ion source are directed to interact with the one sample emitter in order to cause the sample in the one sample emitter to be discharged from the one sample emitter and into the mass spectrometer, wherein each of the plurality of sample emitters is a hollow body and the sample is held within the hollow body, and wherein each of the plurality of sample emitters is selected from the group consisting of: a hollow capillary in which a proximal and distal end are open; a hollow capillary in which a proximal end is sealed and a distal end is open; and a steel needle comprising an inner bore.

2. The system according to claim 1 , wherein a proximal portion of the hollow body of each of the plurality of sample emitters is open ended and the ions from the ion source are directed to interact with and enter a proximal end of the hollow body of each of the plurality of sample emitters, thereby causing the sample of each of the plurality of sample emitters to be discharged from a distal end of each of the plurality of sample emitters and into the mass spectrometer.

3. The system according to claim 1 , wherein a proximal portion of the hollow body of each of the plurality of sample emitters is sealed and the ions from the ion source are directed to interact with the proximal end of the hollow body of each of the plurality of sample emitters, thereby causing the sample of each of the plurality of sample emitters to be discharged from a distal end of each of the plurality of sample emitters and into the mass spectrometer.

4. The system according to claim 1 , wherein the ion source is an electrospray source.

5. The system according to claim 1 , wherein the ion source is an plasma discharge source.

6. The system according to claim 1 , wherein the mass spectrometer is a bench-top or miniature mass spectrometer.

7. A method for analyzing a sample, the method comprising:
aligning an ion source with one of a plurality of sample emitters, each comprising a sample;
generating ions from an ion source;
directing the ions to interact with the one of the plurality sample emitters in order to cause the sample in the one of the plurality sample emitters be discharged from the one of the plurality sample emitters, wherein each of the plurality of sample emitters is a hollow body and the sample is held within the hollow body, and wherein each of the plurality of sample emitters is selected from the group consisting of: a hollow capillary in which a proximal and distal end are open; a hollow capillary in which a proximal end is sealed and a distal end is open; and a steel needle comprising an inner bore;
analyzing the discharged sample in a mass spectrometer; and
repeating the method steps wherein the ion source is aligned with a second of a plurality of sample emitters in order to analyze a second sample in the second of the plurality of sample emitters.

8. The method according to claim 7 , wherein a proximal portion of the hollow body of each of the plurality of sample emitters is open ended and the ions from the ion source are directed to interact with and enter a proximal end of the hollow body of each of the plurality of sample emitters, thereby causing the sample of each of the plurality of sample emitters to be discharged from a distal end of each of the plurality of sample emitters and into the mass spectrometer.

9. The method according to claim 7 , wherein a proximal portion of the hollow body of each of the plurality of sample emitters is sealed and the ions from the ion source are directed to interact with the proximal end of the hollow body of each of the plurality of sample emitters, thereby causing the sample of each of the plurality of sample emitters to be discharged from a distal end of each of the plurality of sample emitters and into the mass spectrometer.

10. The method according to claim 7 , wherein the ion source is an electrospray source.

11. The method according to claim 7 , wherein the ion source is an plasma discharge source.

12. The method according to claim 7 , wherein the sample is a biological sample.

Assignees

• Purdue Research Foundation

Inventors

• Cooks Robert Graham
• Li Anyin
• Hollerbach Adam

Classifications

• H01J49/167

  Capillaries and nozzles specially adapted therefor; (electrostatic spraying per se B05B5/00) · CPC title

• H01J49/165 Primary

  Electrospray ionisation · CPC title

• H01J49/04

  Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components · CPC title

• H01J49/00

  Particle spectrometers or separator tubes · CPC title

• H01J49/0409 Primary

  Sample holders or containers (containers for retaining a material to be analyzed, B01L3/50, for DNA, C12Q1/6834, for biological materials, G01N33/543) · CPC title