Methods, reagents and cells for biosynthesizing compounds
US-9896702-B2 · Feb 20, 2018 · US
Bioconversion process for producing nylon-7, nylon-7,7 and polyesters
US10577634B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10577634-B2 |
| Application number | US-201715462975-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 20, 2017 |
| Priority date | Jun 30, 2011 |
| Publication date | Mar 3, 2020 |
| Grant date | Mar 3, 2020 |
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- Title
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Abstract
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Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.
First claim
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We claim: 1. A method of converting a compound, the method comprising: providing a recombinant host cell expressing a heterologous thioesterase that catalyzes the thioester hydrolysis of pimeloyl-CoA (PCoA) to pimelic acid (PA), wherein the thioesterase is in EC 3.1.2.-; and contacting PCoA with the thioesterase, wherein PA is produced. 2. The method of claim 1 , wherein the thioesterase in EC 3.1.2.- is a thioesterase in EC 3.1.2.18, EC 3.1.2.19, or EC 3.1.2.20, or a thioesterase encoded by a yciA gene. 3. The method of claim 1 , wherein the PCoA is produced by a pathway in the recombinant host cell. 4. The method of claim 1 , further comprising contacting the PA with a carboxylic acid reductase that catalyzes the carboxylic acid reduction of PA to pimelic acid semialdehyde (PAS), wherein PAS is produced. 5. The method of claim 4 , wherein the carboxylic acid reductase that catalyzes the carboxylic acid reduction of PA to PAS is a carboxylic acid reductase in EC 1.2.99.-. 6. The method of claim 5 , wherein the carboxylic acid reductase in EC 1.2.99.- is a carboxylic acid reductase in EC 1.2.99.6 or a carboxylic acid reductase encoded by a car gene. 7. The method of claim 4 , wherein the carboxylic acid reductase that catalyzes the carboxylic acid reduction of PA to PAS is: (i) isolated; (ii) in a cell lysate or partially purified cell lysate; or (iii) expressed in the recombinant host cell. 8. A method of converting a compound, the method comprising: providing a recombinant host cell expressing a heterologous thioesterase that catalyzes the thioester hydrolysis of pimeloyl-CoA (PCoA) to pimelic acid (PA), wherein the thioesterase is in EC 3.1.2.- or is a thioesterase encoded by a yciA gene; and contacting PCoA with the thioesterase, wherein PA is produced. 9. The method of claim 8 , wherein the PCoA is produced by a pathway in the recombinant host cell. 10. The method of claim 8 , further comprising contacting the PA with a carboxylic acid reductase that catalyzes the carboxylic acid reduction of PA to pimelic acid semialdehyde (PAS), wherein PAS is produced. 11. The method of claim 10 , wherein the carboxylic acid reductase that catalyzes the carboxylic acid reduction of PA to PAS is a carboxylic acid reductase in EC 1.2.99.-. 12. The method of claim 11 , wherein the carboxylic acid reductase in EC 1.2.99.- is a carboxylic acid reductase in EC 1.2.99.6 or a carboxylic acid reductase encoded by a car gene. 13. The method of claim 10 , wherein the carboxylic acid reductase that catalyzes the carboxylic acid reduction of PA to PAS is: (i) isolated; (ii) in a cell lysate or partially purified cell lysate; or (iii) expressed in the recombinant host cell.
Assignees
Inventors
Classifications
Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids · CPC title
Acyl-CoA hydrolase (3.1.2.20) · CPC title
acting on ester bonds (3.1) · CPC title
6-Carboxyhexanoate--CoA ligase (6.2.1.14) · CPC title
Hydroxy-carboxylic acids · CPC title
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- What does patent US10577634B2 cover?
- Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.
- Who is the assignee on this patent?
- Invista North America Sarl
- What technology area does this patent fall under?
- Primary CPC classification C12P7/44. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 03 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 5 related publications on this page (citations in our corpus or others sharing the same primary CPC).