Biocompatible smart responsive scaffold having interconnected pores

Patent metadata

Field	Value
Publication number	US-10568986-B2
Application number	US-201415106468-A
Country	US
Kind code	B2
Filing date	Dec 19, 2014
Priority date	Dec 19, 2013
Publication date	Feb 25, 2020
Grant date	Feb 25, 2020

Abstract

A polymeric scaffold contains pendant liquid crystal side chains and has fully interconnected pores. Such a polymeric scaffold will preferably be 3D in nature and elastomeric, biocompatible and biodegradable. Such 3D liquid crystal elastomer (LCE) scaffolds can be used for various biomedical applications, including cell culture applications. A method for the production of such a polymeric scaffold containing liquid crystals and having interconnected pores is also disclosed that uses a metal foam sacrificial template as a scaffold to produce the polymeric smart response scaffold of the present invention. Consistent and controlled pore sizes result from etching the sacrificial metal foam template away from the polymeric scaffold, permitting the incorporation of growth factors, when needed, for enhancing cell viability and proliferation.

First claim

What is claimed is:

1. A polymeric scaffold having interconnected pores and comprising a plurality of cross-linked star block copolymers, each star block copolymer having a plurality of pendant liquid crystal side chains.

2. The polymeric scaffold as claimed in claim 1 , wherein each cross-linked star block copolymer comprises:
a core derived from a polyol; and
a plurality of arms of random block copolymers comprising:
one or more polymer blocks derived from halide-substituted lactone monomers;
one or more polymer blocks derived from lactone monomers or isomers thereof; and
one or more polymer blocks derived from lactide monomers,
wherein at least one of the one or more polymer blocks derived from the halide-substituted lactone monomers of each arm contains a liquid crystal pendant from the polymer block at the position where the halide was originally substituted.

3. The polymeric scaffold as claimed in claim 1 , wherein the interconnected pores have a diameter ranging from about 100 microns to about 500 microns.

4. The polymeric scaffold as claimed in claim 1 , wherein the scaffold is elastomeric.

5. The polymeric scaffold as claimed in claim 1 , wherein the scaffold is biocompatible.

6. The polymeric scaffold as claimed in claim 1 , wherein the scaffold is biodegradable.

7. The polymeric scaffold as claimed in claim 2 , wherein the liquid crystal is selected from the group consisting of a cholesterol-based chiral nematic liquid crystal and derivatives thereof, a cholesteryl liquid crystal and derivatives thereof; and a 3,4-difluoropentyl-bicyclohexyl-base nematic liquid crystal and derivatives thereof.

8. The polymeric scaffold as claimed in claim 1 , wherein the polymeric scaffold further includes a plurality of pores that are not interconnected, and that range in diameter of from 5 microns to 500 microns.

9. The polymeric scaffold as claimed in claim 1 , wherein said cross-linked star block polymers are crosslinked using a compound that is biocompatible and biodegradable with said star block copolymers.

10. The polymeric scaffold as claimed in claim 9 , wherein said compound is selected from the group consisting of 2,2-Bis(1-caprolactone-4-yl) propane (BCP) and derivatives thereof, and Bis-caprolactone with oligoethylene glycol spacer and derivatives thereof.

11. The polymeric scaffold as claimed in claim 1 , wherein the polymeric scaffold includes interconnected pores and interconnected channels, the interconnected pores forming hollow passages within the framework of the polymeric scaffold.

12. The polymeric scaffold as claimed in claim 1 , wherein the scaffold is adaptable for use in combination with stem cells for tissue engineering and cell delivery.

13. The polymeric scaffold as claimed in claim 1 , wherein the scaffold is a biocompatible, smart responsive scaffold responsive to external stimuli.

14. A method for the production of a polymeric scaffold having interconnected pores and comprising a plurality of cross-linked star block copolymers, each star block copolymer having a plurality of pendant liquid crystal side chains, the method comprising:
providing a metal foam scaffold template;
casting a liquid crystal-containing polymeric material around the metal foam scaffold template;
crosslinking the casted liquid crystal-containing polymeric material and metal foam scaffold template to provide a polymeric scaffold around the metal foam scaffold template to form a crosslinked scaffold; and
etching the metal foam scaffold template to remove the metal foam scaffold template embedded within the crosslinked scaffold and to provide a polymeric scaffold and having interconnected pores and comprising a plurality of cross-linked star block copolymers, each star block copolymer having a plurality of pendant liquid crystal side chains, where the metal foam scaffold template was removed.

15. The method as claimed in claim 14 , wherein the step of casting includes the step of dipping the metal foam scaffold template into the liquid crystal-containing polymeric material.

16. The method as claimed in claim 15 , wherein the step of casting further includes removing the metal foam scaffold template from the liquid crystal-containing polymeric material before completely crosslinking so as to provide channels within the polymeric scaffold upon crosslinking, the channels being defined by the voids within the metal foam scaffold template.

17. The method as claimed in claim 14 , wherein the step of casting includes the step of immersing the metal foam scaffold template into the liquid crystal-containing polymeric material.

18. The method as claimed in claim 14 , wherein the step of casting includes the step of pouring the liquid crystal-containing polymeric material over the metal foam scaffold template.

19. The method as claimed in claim 14 , wherein the step of crosslinking includes crosslinking with a crosslinking agent to form a thermoset elastomer, wherein the crosslinking agent is selected from the group consisting of 2,2-bis(1-caprolactone-4-yl) propane (BCP) and derivatives thereof, and bis-caprolactone with oligoethylene glycol spacer and derivatives thereof.

20. The method as claimed in claim 19 , wherein the step of crosslinking further includes heating the casted liquid crystal-containing polymeric material and metal foam scaffold template to at least 140° C.

21. The method as claimed in claim 14 , wherein the step of etching including removing the metal foam scaffold template by immersing the crosslinked scaffold in a saturated etchant solution.

22. The method as claimed in claim 21 , wherein the etchant solution is a FeCl 3 solution.

23. The method as claimed in claim 14 , wherein each star block copolymer comprises:
a core derived from a polyol; and
a plurality of arms of random block copolymers comprising:
one or more polymer blocks derived from halide-substituted lactone monomers;
one or more polymer blocks derived from lactone monomers or isomers thereof; and
one or more polymer blocks derived from lactide monomers,
wherein at least one of the one or more polymer blocks derived from the halide-substituted lactone monomers of each arm contains a liquid crystal pendant from the polymer block at the position where the halide was originally substituted.

24. The method as claimed in claim 14 , wherein the diameter of the interconnected pores of the polymeric scaffold is determined by the thickness of each metal fiber forming the metal foam scaffold template.

25. The method as claimed in claim 14 , wherein the metal in the metal foam scaffold template is nickel or iron oxide.

26. The polymeric scaffold as claimed in claim 1 , wherein the pendant liquid crystal side chains are biocompatible.

Assignees

• Univ Kent State Ohio

Inventors

• Hegmann Elda
• Hegmann Torsten
• Gao Yunxiang

Classifications

• A61L2400/08

  Methods for forming porous structures using a negative form which is filled and then removed by pyrolysis or dissolution · CPC title

• C09K2219/00

  Aspects relating to the form of the liquid crystal [LC] material, or by the technical area in which LC material are used · CPC title

• C12N2533/30

  Synthetic polymers · CPC title

• C08J2201/0442

  the inorganic phase being a metal, its oxide or hydroxide · CPC title

• A61L27/18 Primary

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds · CPC title