Capacitance spectroscopic method and electrode

Patent metadata

Field	Value
Publication number	US-10564154-B2
Application number	US-201414912017-A
Country	US
Kind code	B2
Filing date	Jun 25, 2014
Priority date	Aug 12, 2013
Publication date	Feb 18, 2020
Grant date	Feb 18, 2020

Abstract

Capacitance spectroscopic method and electrode The application relates to methods and electrodes for electrochemical detection of target species by capacitance spectroscopy. The method is simple, frequency optimised and extremely sensitive to low concentrations of target species. The electrodes of the invention can easily be reused and are ideally suited for use in point-of-care diagnostics.

First claim

The invention claimed is:

1. An electrode for use in the electrochemical detection of a target species, the electrode comprising an electrode surface on which first moieties and second moieties are disposed,
wherein said first moieties and said second moieties are separate moieties and wherein each of said first moieties comprises a redox active probe and each of said second moieties comprises a receptor capable of binding selectively to said target species; and
wherein the ratio of first moieties to second moieties disposed on said surface of said electrode is from 95:5 to 75:25.

2. An electrochemical spectrometer comprising an electrode of claim 1 .

3. The electrode according to claim 1 , wherein said redox active probe is ferrocene.

4. The electrode according to claim 1 , wherein said receptor is selected from the group consisting of antibodies, antibody fragments, aptamers, oligosaccharides, peptides and proteins.

5. The electrode according to claim 1 , wherein said target species is selected from the group consisting of CRP protein, insulin and a marker of one or more of neurodegeneration, cancer, myocardial infarction, diabetes and general trauma.

6. The electrode according to claim 1 , wherein said first moieties have the formula A 1 -L 1 -RAP, wherein A 1 is a moiety bound to the surface of the electrode, L 1 is a linker moiety and RAP is said redox active probe.

7. The electrode according to claim 1 , wherein said second moieties have the formula A 2 -L 2 -REC, wherein A 2 is a moiety bound to the surface of the electrode, L 2 is a linker moiety and REC is said receptor.

Assignees

• Univ Oxford Innovation Ltd
• Univ Estadual Paulista “Julio De Mesquita Filho”—Unesp
• Univ Estadual Paulista “Julio De Mesquita Filho”—Un Esp

Inventors

• Davis Jason
• Bueno Paulo Roberto

Classifications

• G01N33/54353

  with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393) · CPC title

• G01N27/3277

  being a redox reaction, e.g. detection by cyclic voltammetry (voltammetry per se G01N27/42, G01N27/48) · CPC title

• G01N27/27

  Association of two or more measuring systems or cells, each measuring a different parameter, where the measurement results may be either used independently, the systems or cells being physically associated, or combined to produce a value for a further parameter · CPC title

• G01N27/3276

  being a hybridisation with immobilised receptors (using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68) · CPC title

• G01N33/5438 Primary

  Electrodes · CPC title