Microorganism culture sheet

Patent metadata

Field	Value
Publication number	US-10563167-B2
Application number	US-201113575906-A
Country	US
Kind code	B2
Filing date	Jan 26, 2011
Priority date	Jan 29, 2010
Publication date	Feb 18, 2020
Grant date	Feb 18, 2020

Abstract

Provided is a microorganism culture sheet having an excellent efficiency for collection of environmental microorganisms and a method for measuring the environmental microorganisms with an excellent collection efficiency. Provided is a microorganism culture sheet including a base sheet, a dry culture layer formed on the base sheet, and a cover sheet covering the dry culture layer, wherein an adhesive layer and a release film are laminated on the inner surface of the cover sheet. Because the environmental microorganisms are collected using the adhesive layer, an excellent collection efficiency is provided.

First claim

The invention claimed is:

1. A microorganism culture sheet consisting of a base sheet, a dry culture layer formed on said base sheet, a frame layer, and a cover sheet directly covering said dry culture layer, wherein:
said dry culture layer is formed in a circle shape by patterning a medium suspension consisting of a gelling agent dispersed in an alcohol, polyvinyl pyrrolidone dissolved in the alcohol, and nutrients for culturing microorganisms on said base sheet and then drying to remove the alcohol, said dry culture layer has a circumference and a height, and said gelling agent is selected from the group consisting of carrageenan, guar gum, xanthan gum, locust bean gum, and algin;
the dry culture layer is present on the base sheet in an amount of 5 to 400 g/m 2 ;
said frame layer is formed in a hydrophobic resin on said base sheet around said dry culture layer; and
said cover sheet has an inner surface, an adhesive layer on the inner surface, and a release film laminated on the adhesive layer so that upon removal of the release film, the adhesive layer is capable of collecting a bacterium.

2. The microorganism culture sheet according to claim 1 , wherein said adhesive layer has a thickness of 1 to 100 μm.

3. The microorganism culture sheet according to claim 1 , wherein said frame layer is higher than the height of said dry culture layer by 100 to 1200 μm.

4. The microorganism culture sheet according to claim 1 , wherein said base sheet and/or cover sheet are/is made of a transparent plastic sheet.

5. The microorganism culture sheet of claim 1 wherein the dry culture layer contains substantially no water.

6. The microorganism culture sheet of claim 1 , wherein said alcohol is at least one selected from the group consisting of methanol and ethanol.

7. A microorganism culture kit comprising said microorganism culture sheet according to claim 1 and a gelling solution.

8. A microorganism culture sheet consisting of a base sheet, a dry culture layer formed on said base sheet, a frame layer, and a cover sheet directly covering said dry culture layer, wherein:
said dry culture layer is formed in a circle shape by patterning a medium suspension consisting of a gelling agent dispersed in an alcohol, polyvinyl pyrrolidone dissolved in the alcohol, nutrients for culturing microorganisms, and at least one other component selected from the group consisting of chromogenic indicator, selective agent, substrate, and buffering agent, on said base sheet and then drying to remove the alcohol, said dry culture layer has a circumference and a height, and said gelling agent is selected from the group consisting of carrageenan, guar gum, xanthan gum, locust bean gum, and algin;
the dry culture layer is present on the base sheet in an amount of 5 to 400 g/m 2 ;
said frame layer is formed in a hydrophobic resin on said base sheet around said dry culture layer; and
said cover sheet has an inner surface, an adhesive layer on the inner surface, and a release film laminated on the adhesive layer so that upon removal of the release film, the adhesive layer is capable of collecting a bacterium.

9. A method for culturing a bacterium collected on said adhesive layer of said microorganism culture sheet in said microorganism culture kit according to claim 7 , wherein said method comprising the steps of collecting said bacterium in said adhesive layer, turning said dry culture layer of said microorganism culture sheet into a gel by adding said gelling solution and covering with said adhesive layer of said cover sheet, and culturing after the completion of gelling.

10. The method for culturing a bacterium according to claim 9 , comprising bringing said adhesive layer into contact with a test surface to collect said bacterium.

11. The method for culturing a bacterium according to claim 9 , comprising leaving said adhesive layer uncovered for a prescribed time to collect falling bacterium.

Assignees

• Waku Shin Ichi
• Miyagi Michiko
• Saito Rui
• Ueki Tetsuji
• Baba Takuma
• Kinoshita Mai
• Dainippon Printing Co Ltd

Inventors

• Waku Shin-Ichi
• Miyagi Michiko
• Saito Rui
• Ueki Tetsuji
• Baba Takuma
• Kinoshita Mai

Classifications

• C12Q1/04

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• G01N2001/2833

  Collecting samples on a sticky, tacky, adhesive surface · CPC title

• C12M23/22

  Transparent or translucent parts (glassware for laboratory use B01L3/00) · CPC title

• C12M1/14

  with means providing thin layers or with multi-level trays · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title