Method of purifying a biological composition and article therefor

Patent metadata

Field	Value
Publication number	US-10562997-B2
Application number	US-201615549268-A
Country	US
Kind code	B2
Filing date	Mar 17, 2016
Priority date	Mar 24, 2015
Publication date	Feb 18, 2020
Grant date	Feb 18, 2020

Abstract

A method of purifying a biological composition includes: disposing loose cationic ligand-functionalized staple fibers and a biological composition within a mixing volume of a vessel; agitating the biological composition and the loose cationic ligand-functionalized staple fibers while they are in intimate contact with each other within the mixing volume to provide modified fibers and a purified biological composition; and separating at least a portion of the purified biological composition from the modified fibers and any loose cationic ligand-functionalized staple fibers with which it is in contact. The loose cationic ligand-functionalized staple fibers have a modified surface layer comprising a grafted acrylic polymer comprising 10 to 100 percent by weight of a cationically-ionizable monomer unit. An article for purifying a biological composition includes: a vessel having a mixing volume disposed therein; and the loose cationic ligand-functionalized staple fibers disposed within the mixing volume.

First claim

What is claimed is:

1. A method of purifying a biological composition, the method comprising:
a) disposing loose cationic ligand-functionalized staple fibers and a biological composition within a mixing volume of a vessel, wherein the loose cationic ligand-functionalized staple fibers have a modified surface layer comprising a grafted acrylic polymer comprising 10 to 100 percent by weight of a cationically-ionizable monomer unit, and wherein at least a portion of the loose cationic ligand-functionalized staple fibers are fibrillated high-density polyethylene fibers;
b) agitating the biological composition and the loose cationic ligand-functionalized staple fibers while they are in intimate contact with each other within the mixing volume to provide modified fibers and a purified biological composition; and
c) separating at least a portion of the purified biological composition from the modified fibers and any loose cationic ligand-functionalized staple fibers with which it is in contact.

2. The method of claim 1 , wherein the loose cationic ligand-functionalized staple fibers are disposed within the mixing volume before the biological composition is disposed within the mixing volume.

3. The method of claim 1 , wherein step b) is accomplished at least in part using a static mixer.

4. The method of claim 1 , wherein the biological composition contains a synthetic polymer flocculant.

5. The method of claim 1 , wherein the grafted acrylic polymer further comprises a multifunctional monomer unit.

6. The method of claim 1 , wherein the grafted acrylic polymer further comprises 0.1 to 90 percent by weight of at least one nonionizable hydrophilic monomer unit.

7. The method of claim 6 , wherein the nonionizable hydrophilic monomer unit comprises an N-vinyllactam having from 4 to 6 carbon atoms.

8. The method of claim 1 , wherein the cationically-ionizable monomer unit is a divalent monomer unit represented by the formula:

wherein:
R 1 is H or an alkyl group having from 1 to 4 carbon atoms;
R 2 is a divalent alkylene group optionally substituted by a catenary carbonyloxy, carbonylamino, oxycarbonylamino, or ureylene divalent linking group;
each R 3 independently is H or an alkyl group having from 1 to 4 carbon atoms;
R 4 is H or an alkyl group having from 1 to 4 carbon atoms or —N(R 3 ) 2 ; and
X 1 is —O— or —NR 3 —.

9. The method of claim 1 , wherein the cationically-ionizable monomer unit comprises a divalent monomer unit represented by the formula:

wherein:
R 1 is H or an alkyl group having from 1 to 4 carbon atoms;
R 2 is a divalent alkylene group optionally substituted by a catenary carbonyloxy, carbonylamino, oxycarbonylamino, or ureylene divalent linking group; and
each R 3 independently is H or an alkyl group having from 1 to 4 carbon atoms.

10. The method of claim 1 , wherein the vessel comprises a sealed specimen bag.

11. The method of claim 1 , wherein step c) includes depositing at least a portion of the modified fibers and any loose cationic ligand-functionalized staple fibers on a porous substrate.

12. An article for purifying a biological composition, the article comprising:
a vessel having a mixing volume disposed therein; and
loose cationic ligand-functionalized staple fibers disposed within the mixing volume, wherein the loose cationic ligand-functionalized staple fibers have a modified surface layer comprising a grafted acrylic polymer comprising 10 to 100 percent by weight of at least one cationically-ionizable acrylic monomer unit, and wherein at least a portion of the loose cationic ligand-functionalized staple fibers are fibrillated high-density polyethylene fibers.

13. The article of claim 12 , wherein the grafted acrylic polymer comprises a multifunctional monomer unit.

14. The article of claim 13 , wherein the grafted acrylic polymer further comprises 0.1 to 90 percent by weight of at least one nonionizable hydrophilic monomer unit.

15. The article of claim 14 , wherein the nonionizable hydrophilic monomer unit comprises an N-vinyllactam having from 4 to 6 carbon atoms.

16. The article of claim 12 , wherein the cationically-ionizable acrylic monomer unit is represented by the formula:

wherein:
R 1 is H or an alkyl group having from 1 to 4 carbon atoms;
R 2 is a divalent alkylene group optionally substituted by a catenary carbonyloxy, carbonylamino, oxycarbonylamino, or ureylene divalent linking group;
each R 3 is independently H or an alkyl group having from 1 to 4 carbon atoms;
R 4 is H or an alkyl group having from 1 to 4 carbon atoms or —N(R 3 ) 2 ; and
X 1 is —O— or —NR 3 —.

17. The article of claim 12 , wherein the cationically-ionizable monomer unit comprises a divalent monomer unit represented by the formula:

wherein:
R 1 is H or an alkyl group having from 1 to 4 carbon atoms;
R 2 is a divalent alkylene group optionally substituted by a catenary carbonyloxy, carbonylamino, oxycarbonylamino, or ureylene divalent linking group; and
each R 3 independently is H or an alkyl group having from 1 to 4 carbon atoms.

18. The article of claim 12 , wherein the biological composition contains a synthetic polymer flocculant.

Assignees

• 3M Innovative Properties Co

Inventors

• Vail Andrew W
• Rasmussen Jerald K
• Schmidt John J
• Ozcam Ali E
• Hester Jonathan F
• Jellum Gregory M

Classifications

• D06M14/28

  of macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds · CPC title

• G01N2001/4088

  filtration · CPC title

• C12N5/00 Primary

  Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; (plant reproduction by tissue culture techniques A01H4/00) · CPC title

• D06M15/3562

  containing nitrogen · CPC title

• D06M15/267

  of unsaturated carboxylic esters having amino or quaternary ammonium groups · CPC title