Mass spectrometer, system comprising the same, and methods for determining isotopic anatomy of compounds

Patent metadata

Field	Value
Publication number	US-10559457-B2
Application number	US-201816206951-A
Country	US
Kind code	B2
Filing date	Nov 30, 2018
Priority date	Oct 10, 2012
Publication date	Feb 11, 2020
Grant date	Feb 11, 2020

Abstract

A first mass spectrometer includes a first introduction device configured to select between a reference material and a first portion of an analyte and introduce the selected one of the reference material or the first portion of the analyte to an ion source, the first mass spectrometer being configured to provide third molecular analyte ions to a detector at a first mass resolution of about 30,000 or greater. A system includes the first mass spectrometer and a second mass spectrometer. A method for determining the isotopic composition of an analyte in a sample includes converting a first portion of the analyte to first molecular analyte ions, filtering out second molecular analyte ions, filtering out third molecular analyte ions, detecting two or more of the third molecular analyte ions at a mass resolution of about 30,000 or greater to determine the isotopic composition of at least a portion of the analyte.

First claim

What is claimed is:

1. A method for determining the isotopic composition of an analyte in a sample, the method comprising:
using a first mass spectrometer comprising a single-collector and having a mass-resolution of about 30,000 or greater to measure ratios of ion beams at each cardinal mass of the analyte to produce first molecular analyte ion data;
using a second mass spectrometer comprising a multi-collector to measure relative abundances of closely-adjacent ions at different cardinal masses of the analyte to produce second molecular analyte ion data;
utilizing the first molecular analyte ion data to identify proportions of isotopologues that contribute to signal intensity at each cardinal mass measured with the second mass spectrometer; and
utilizing the proportions of the isotopologues that contribute to the signal intensity at each cardinal mass to compensate for unresolved detected molecular analyte species and calculate an abundance of one or more isotopic species of interest measured with the second mass spectrometer.

2. The method of claim 1 , wherein the analyte is a high molecular mass compound.

3. The method of claim 1 , wherein the analyte is further analyzed using the first mass spectrometer.

4. The method of claim 3 , wherein the first mass spectrometer has a mass-resolution of up to 80,000 to 100,000.

5. The method of claim 3 , wherein the first mass spectrometer comprises:
a first ion travel path;
a first introduction device configured to select between a reference material and a first portion of the analyte and introduce the selected one of the reference material or the first portion of the analyte to a first ion source, the first ion source having a first entrance slit, the first entrance slit having a first width, the first ion source configured to convert the reference material or the first portion of the analyte to first molecular analyte ions and to guide the first molecular analyte ions along the first ion travel path, each of the first molecular analyte ions having a momentum;
a first momentum filter positioned downstream from the first ion source and configured to receive the first molecular analyte ions, the first momentum filter having a first radius of curvature along the first ion travel path, the first momentum filter configured to filter out second molecular analyte ions from the first molecular analyte ions according to their momenta, each of the second molecular analyte ions having an energy level;
a first energy filter positioned downstream from the first momentum filter and configured to receive the second molecular analyte ions, the first energy filter having a second radius of curvature along the first ion travel path, the first energy filter configured to filter out third molecular analyte ions from the second molecular analyte ions according to their energy levels; and
a detector positioned downstream of the first energy filter and configured to receive the third molecular analyte ions,
wherein the width of the first entrance slit and the first and second radii of curvature are selected to provide a first mass resolution at the detector of about 30,000 or greater.

6. The method of claim 5 , wherein the detector comprises the single-collector and the single-collector is configured to detect third molecular analyte ions.

7. The method of claim 5 , wherein the first introduction device is configured to receive the first portion of the analyte as a gas phase analyte.

8. The method of claim 5 , wherein:
the analyte is a gas phase analyte and the first introduction device comprises a first inlet coupled to a sample reservoir configured to accommodate the gas phase analyte, the reference material is a gas phase reference material and the first introduction device comprises a second inlet coupled to a reference reservoir configured to accommodate the gas phase reference material,
the sample reservoir is configured to accommodate the gas phase analyte at a first pressure,
the reference reservoir is configured to accommodate the gas phase reference material at a second pressure, and
the first and second pressures are the same.

9. The method of claim 5 , wherein the first introduction device is configured to receive the first portion of the analyte entrained in a flow of inert gas.

10. The method of claim 5 , wherein the first momentum filter is configured to produce a magnetic field, the first energy filter is configured to produce an electric field, and the mass spectrometer is configured to vary masses of the third molecular analyte ions detected at the detector by maintaining a strength of the magnetic field of the first momentum filter at a set value and varying a strength of the electric field of the first energy filter.

11. The method of claim 5 , wherein:
the first momentum filter is configured to produce a magnetic field,
the first energy filter is configured to produce an electric field,
the mass spectrometer is configured to vary a first set of masses of the third molecular analyte ions detected at the detector by maintaining a first strength of the magnetic field of the first momentum filter at a first set value and varying a strength of the electric field of the first energy filter, and
the mass spectrometer is configured to vary a second set of masses of the third molecular analyte ions detected at the detector by maintaining a second strength of the magnetic field of the first momentum filter at a second set value and varying a strength of the electric field of the first energy filter.

12. The method of claim 1 , wherein the second mass spectrometer is a gas source, double focusing, high resolution multi-collector instrument.

13. The method of claim 1 , wherein the second mass spectrometer comprises an inlet system of a gas source isotopic ratio mass spectrometer and an analyser and detector array.

14. The method of claim 1 , wherein the second mass spectrometer is a dual-inlet, multi-collector instrument.

15. The method of claim 1 , wherein the second mass spectrometer is capable of measuring ions having masses up to 300 amu.

Assignees

• California Inst Of Techn
• Thermo Fisher Scient Bremen Gmbh

Inventors

• Eiler John M
• Schwieters Johannes

Classifications

• H01J49/26 Primary

  Mass spectrometers or separator tubes · CPC title

• H01J49/0009

  Calibration of the apparatus · CPC title

• H01J49/0031 Primary

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title

• H01J49/04

  Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components · CPC title

• H01J49/326

  with magnetic and electrostatic sectors of 90 degrees · CPC title