Zirconium-89 oxine complex as a cell labeling agent for positron emission tomography

Patent metadata

Field	Value
Publication number	US-10556916-B2
Application number	US-201515300883-A
Country	US
Kind code	B2
Filing date	Apr 1, 2015
Priority date	Apr 1, 2014
Publication date	Feb 11, 2020
Grant date	Feb 11, 2020

Abstract

The invention provides a method of preparing a 89 Zr-oxine complex of the formula. The invention also provides a method of labeling a cell with the 89 Zr-oxine complex and a method for detecting a biological cell in a subject comprising administering the 89 Zr-oxine complex to the subject.

First claim

The invention claimed is:

1. A method of labeling a cell or microorganism with 89 Zr-oxine complex comprising contacting the cell or microorganism with a 89 Zr-oxine complex of the formula

in a buffer solution at 26° C. or below, wherein the 89 Zr-oxine complex permeabilizes the cell membrane of the cell or microorganism.

2. The method of claim 1 wherein the 89 Zr-oxine complex-labeled cells or microorganisms are washed free of non-internalized 89 Zr.

3. The method of claim 1 , wherein the cell is a healthy cell.

4. The method of claim 3 , wherein the healthy cell is a T cell, a natural killer (NK) cell, a dendritic cell, a macrophage, a monocyte, a B cell, a myeloid cell, a platelet, a stem cell, a progenitor cell, a mesenchymal cell, an epithelial cell, a neural cell, a skeletal myoblast, or a pancreatic islet cell.

5. The method of claim 1 , wherein the buffer solution further comprises a second labeling agent, whereby the cell or microorganism is labeled with both 89 Zr-oxine complex and the second labeling agent.

Assignees

• Us Health

Inventors

• Sato Noriko
• Wu Haitao
• Griffiths Gary L
• Choyke Peter L

Classifications

• C12Q1/16

  using radioactive material · CPC title

• A61K51/1203

  in a form not provided for by groups A61K51/1206 - A61K51/1296, e.g. cells, cell fragments, viruses, virus capsides, ghosts, red blood cells, viral vectors · CPC title

• G01N2458/30

  Electrochemically active labels · CPC title

• G01N33/60

  involving radioactive labelled substances · CPC title

• C07B59/004

  Acyclic, carbocyclic or heterocyclic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen, sulfur, selenium or tellurium · CPC title