Complex of a glucopyranosyl derivative and preparation method and use thereof

Patent metadata

Field	Value
Publication number	US-10555930-B2
Application number	US-201615778220-A
Country	US
Kind code	B2
Filing date	Nov 28, 2016
Priority date	Nov 27, 2015
Publication date	Feb 11, 2020
Grant date	Feb 11, 2020

Abstract

This present invention relates to a complex of a glucopyranosyl derivative and L-pyroglutamic acid as a sodium dependent glucose cotransporter (SGLT) inhibitor, and preparation processes thereof, and a pharmaceutical composition containing the complex, and their uses in the manufacture of a medicament for treating diabetes and/or diabetes-related diseases.

First claim

What is claimed is:

1. A complex having Formula (IA):

or a hydrate thereof, wherein the complex comprises a compound having Formula (I-n) and L-pyroglutamic acid at a mole ratio of 1:1,

wherein the complex is in a crystalline form, and wherein the crystalline form has an X-ray powder diffraction pattern:
comprising peaks at scattering angles (2θ) of 3.61°±0.2°, 13.35°±0.2°, 17.84°±0.2°, 18.22°±0.2°, 19.92°±0.2° and 21.43°±0.2°; or
comprising peaks at scattering angles (2θ) of 3.61°±0.2°, 7.14°±0.2°, 13.35°±0.2°, 17.84°±0.2°, 18.22°±0.2°, 19.92°±0.2°, 21.43°±0.2° and 22.70°±0.2°; or
comprising peaks at scattering angles (2θ) of 3.61°±0.2°, 7.14°±0.2°, 11.44°±0.2°, 11.84°±0.2°, 13.35°±0.2°, 16.33°±0.2°, 16.71°±0.2°, 17.16°±0.2°, 17.84°±0.2°, 18.22°±0.2°, 19.92°±0.2°, 21.43°±0.2°, 22.70°±0.2° and 22.96°±0.2°; or
comprising peaks at scattering angles (2θ) of 3.61°±0.2°, 7.14°±0.2°, 11.44°±0.2°, 11.84°±0.2°, 13.35°±0.2°, 16.33°±0.2°, 16.71°±0.2°, 17.16°±0.2°, 17.84°±0.2°, 18.22°±0.2°, 18.52°±0.2°, 19.92°±0.2°, 21.43°±0.2°, 21.74°±0.2°, 22.70°±0.2°, 22.96°±0.2°, 23.75°±0.2°, 24.31°±0.2°, 25.07°±0.2°, 25.84°±0.2°, 26.50°±0.2°, 27.75°±0.2°, 28.61°±0.2°, 29.25°±0.2°, 29.44°±0.2°, 30.17°±0.2°, 30.99°±0.2°, 31.59°±0.2°, 32.40°±0.2°, 32.81°±0.2°, 34.32°±0.2°, 34.79°±0.2°, 35.43°±0.2°, 36.09°±0.2° and 38.03°±0.2°; or

substantially the same as shown in FIG. 2 .

2. The complex of claim 1 , wherein the complex is a hydrate containing 1.25 equivalents of water of crystallization.

3. The complex of claim 1 , wherein the crystalline form has at least one of the following features:
(i) a differential scanning calorimetry thermogram comprising an endothermic peak at 96.9° C.±3° C.;
(ii) a Raman spectrogram comprising absorption peaks at 1454.51 cm −1 ±1 cm −1 , 1303.40 cm −1 ±1 cm −1 , 1183.17 cm −1 ±1 cm −1 , 1012.34 cm −1 ±1 cm −1 and 495.61 cm −1 ±1 cm −1 ; and
(iii) a Fourier transform infrared spectrogram comprising absorption peaks at 3259.22 cm −1 ±5 cm −1 , 2985.55 cm −1 ±5 cm −1 , 2926.65 cm −1 ±5 cm −1 , 1750.08 cm −1 ±2 cm −1 , 1648.90 cm −1 ±2 cm −1 , 1511.90 cm −1 ±2 cm −1 , 1475.81 cm −1 ±2 cm −1 , 1263.43 cm −1 ±2 cm −1 , 1238.92 cm −1 ±2 cm −1 , 1206.04 cm −1 ±2 cm −1 , 1088.08 cm −1 ±2 cm −1 , 1060.72 cm −1 ±2 cm −1 , 1010.97 cm −1 ±2 cm −1 and 821.26 cm −1 ±2 cm −1 .

4. The complex of claim 1 , wherein the crystalline form has at least one of the following features:
(i) a differential scanning calorimetry thermogram substantially the same as shown in FIG. 3 ;
(ii) a Raman spectrogram substantially the same as shown in FIG. 4 ;
(iii) a Fourier transform infrared spectrogram substantially the same as shown in FIG. 5 ; and
(iv) the following unit cell parameters:
unit cell dimension: a=7.4751 (2) Å, b=7.8333 (3) Å, c=49.4417 (19) Å, α=90°, β=90°, γ=90°;
space group: orthogonality, P 2 1 2 1 2 1 ;
cell volume: 2895.04 Å 3 ; and
number of asymmetric units per unit cell (Z): 4.

5. A pharmaceutical composition comprising the complex of claim 1 , optionally, further comprising a pharmaceutically acceptable adjuvant.

6. The pharmaceutical composition of claim 5 further comprising an additional therapeutic agent, wherein the additional therapeutic agent is an anti-diabetic agent other than an SGLT-2 inhibitor, an antihyperglycemic agent, an antiadipositas drug, an antihypertensive agent, an antiplatelet agent, an antiatheroaclerotic drug, a lipid-lowering agent, an anti-inflammatory or a combination thereof.

7. The pharmaceutical composition of claim 6 , wherein the anti-diabetic agent other than an SGLT-2 inhibitor is a biguanide, a sulfonylurea, a glucosidase inhibitor, a PPAR agonist, an αP2 inhibitor, a PPARα/γ dual agonist, a dipeptidyl peptidase IV inhibitor, a glinide, insulin, a glucagon-like peptide-1 inhibitor, a PTP1B inhibitor, a glycogen phosphorylase inhibitor, a glucose-6-phosphatase inhibitor or a combination thereof;
wherein the antihyperglycemic agent is a biguanide, a sulfonylurea, a glucosidase inhibitor, a PPAR agonist, an αP2 inhibitor, a PPARα/γ dual agonist, a dipeptidyl peptidase IV inhibitor, a glinide, insulin, a glucagon-like peptide-1 inhibitor, a PTP1B inhibitor, a glycogen phosphorylase inhibitor, a glucose-6-phosphatase inhibitor or a combination thereof;
wherein the lipid-lowering agent is an MTP inhibitor, an HMGCoA reductase inhibitor, a squalene synthase inhibitor, a fibrate antihyperlipidemic drug, an ACAT inhibitor, a lipoxygenase inhibitor, a cholesterol absorption inhibitor, an ileal Na(+)/bile acid cotransporter inhibitor, an upregulator of LDL receptor activity, a nicotinic antihyperlipidemic drug, a bile acid sequestrant or a combination thereof; or
the lipid-lowering agent is pravastatin, simvastatin, atorvastatin, fluvastatin, cerivastatin, atavastatin, rosuvastatin or a combination thereof.

8. A process of preparing the complex of claim 1 , wherein the process comprises the steps of:
(i) dissolving the compound having Formula (I-n) and L-pyroglutamic acid in a solvent;
(ii) cooling the solution obtained from step (i) to precipitate a solid; and
(iii) separating the solid obtained from step (ii).

9. The process of claim 8 , in step (i), wherein the solvent is a mixture of ethanol and water at a volume ratio from (1:1) to (1:2) or a mixture of isopropanol and water at a volume ratio from (1:1) to (1:2).

10. The process of claim 8 , in step (i), wherein the amount of the solvent is from 1.5 mL to 5 mL per gram of the compound having Formula (I-n).

11. The process of claim 8 , wherein the mole ratio of the compound having Formula (I-n) and L-pyroglutamic acid in step (i) is from (1:2) to (1:5) or from (1:3) to (1:4).

12. The process of claim 8 , wherein the dissolving temperature in step (i) is from 70° C. to 90° C.

13. The process of claim 8 , wherein the cooling in step (ii) is natural cooling at a temperature from 10° C. to 30° C.

14. The process of claim 8 , wherein the separating in step (iii) is filtration under vacuum suction, wherein the filtration under vacuum suction further comprises washing the solid after separation, wherein the solid after separation is washed with a mixture of ethanol and water at a volume ratio from (1:1) to (1:2) or a mixture of isopropanol and water at a volume ratio from (1:1) to (1:2), and wherein the mixture is pre-cooled to a temperature from 20° C. to 0° C.

15. A process of preparing the complex of claim 1 , wherein the process comprises the steps of:
(i) dissolving the compound having Formula (I-n) and L-pyroglutamic acid in a solvent;
(ii) cooling the solution obtained from step (i) to precipitate a solid; and
(iii) separating the solid obtained from step (ii),
wherein,
in step (i),
the solvent is a mixture of ethanol and water at a volume ratio (1:1) to (1:2) or a mixture of isopropanol and water at a volume ratio from (1:1) to (1:2);
the amount of the solvent is from 1.5 mL to 5 mL per gram of the compound having Formula I-n,
the mole ratio of the compound having Formula (I-n) and L-pyroglutamic acid is from (1:3) to (1:4), and
the dissolving temperature is from 70° C. to 90° C.,
in step (ii),
the cooling is natural cooling at a temperature from 10° C. to 30° C.,
in step (iii),
the separating is filtration under vacuum suction, wherein the filtration under vacuum suction further comprises washing the solid after separation, wherein the solid after separation is washed

Assignees

• Sunshine Lake Pharma Co Ltd
• North & South Brother Pharmacy Investment Co Ltd

Inventors

• Tang Pengcho
• Gu Zheng
• Wu Wuyong
• Zhang Zongyuan
• Kang Panpan
• Qu Tong

Classifications

• C07H7/04

  Carbocyclic radicals · CPC title

• C07D207/28

  2-Pyrrolidone-5- carboxylic acids; Functional derivatives thereof, e.g. esters, nitriles · CPC title

• C07D493/08

  Bridged systems · CPC title

• C07H1/00

  Processes for the preparation of sugar derivatives · CPC title

• A61P9/10

  for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis · CPC title