Plant growth-promoting bacteria and methods of use

Patent metadata

Field	Value
Publication number	US-10555532-B2
Application number	US-201414775858-A
Country	US
Kind code	B2
Filing date	Mar 17, 2014
Priority date	Mar 15, 2013
Publication date	Feb 11, 2020
Grant date	Feb 11, 2020

Abstract

The present invention generally relates to bacteriologically pure bacterial cultures of novel strains of plant growth-promoting bacteria, and inoculums comprising the same. The invention is also directed to plant seeds coated with the inoculums, kits comprising the inoculums and methods for stimulating plant growth by applying the biologically pure bacterial culture or the inoculum to a plant, plant seed, or plant growth medium.

First claim

What is claimed is:

1. A method for stimulating plant growth comprising applying a bacterial culture to a plant, plant seed, or plant growth medium, wherein the bacteria in the bacterial culture comprise:
(a) Bacillus mycoides strain BT155 (NRRL No. B-50291);
(b) Bacillus mycoides strain EE118 (NRRL No. B-50918);
(c) Bacillus subtilis strain EE148 (NRRL No. B-50927);
(d) Alcaligenes faecalis strain EE107 (NRRL No. B-50920);
(e) Bacillus mycoides strain EE141 (NRRL No. B-50916);
(f) Bacillus mycoides strain BT46-3 (NRRL No. B-50922);
(g) Bacillus cereus family member strain EE128 (NRRL No. B-50917);
(h) Bacillus thuringiensis strain BT013A (NRRL No. B-50924);
(i) Paenibacillus massiliensis strain BT23 (NRRL No. B-50923);
(j) Bacillus cereus family member strain EE349 (NRRL No. B-50928);
(k) Bacillus subtilis strain EE218 (NRRL No. B-50926);
(l) Bacillus megaterium strain EE281 (NRRL No. B-50925);
(m) salt-tolerant and thiram-resistant Paracoccus sp. strain NC35 (NRRL No. B-50948);
(n) salt-tolerant and thiram resistant Bacillus mycoides strain BT155 (NRRL No. B-50949);
(o) thiram-resistant Bacillus aryabhattai strain CAP53 (NRRL No. B-50946);
(p) thiram-resistant Bacillus thuringiensis strain BT013A (NRRL No. B-50947); or
(q) glyphosate-tolerant Bacillus aryabhattai strain CAP53 (NRRL No. B-50945).

2. A method of claim 1 , wherein the bacteria in the bacterial culture are mutants having all of the identifying characteristics of any of strains (a) through (l), wherein the mutants comprise one or more mutations and retain the ability to promote plant growth.

3. A method of claim 2 , wherein the mutant comprises a salt-tolerant mutant, a thiram-resistant mutant, a glyphosate-tolerant mutant, or a mutant with a combination of mutations to impart salt tolerance, thiram resistance and/or glyphosate tolerance.

4. A method of claim 1 , comprising applying the bacterial culture to plant seeds.

5. A method of claim 1 , wherein the bacterial culture is applied in an inoculum comprising an effective amount of the bacterial culture and an agriculturally acceptable carrier.

6. A method of claim 1 , wherein the bacterial culture is a biologically pure bacterial culture.

7. A method of claim 1 , wherein the bacteria in the bacterial culture comprise Bacillus thuringiensis strain BT013A and the bacteria have a 16S ribosomal RNA sequence having at least about 99% sequence identity with the sequence of SEQ ID NO: 16.

8. A method of claim 1 , wherein the bacteria in the bacterial culture comprise Bacillus thuringiensis strain BT013A.

9. A method of claim 1 , wherein the bacteria in the bacterial culture comprise Bacillus cereus family member strain EE349.

10. A method of claim 1 , wherein the bacterial in the bacterial culture comprise Bacillus cereus family member strain EE349 and the bacteria have a 16S ribosomal RNA sequence having at least about 99% sequence identity with the sequence of SEQ ID NO: 18.

11. A method of claim 5 , wherein the inoculum comprises an effective amount of a mixture comprising at least two of the bacterial cultures.

12. A method of claim 5 , wherein the inoculum further comprises an effective amount of a rhizobacteria.

13. A method of claim 12 , wherein the rhizobacteria comprises Bradyrhizobium genus bacteria, Rhizobium genus bacteria, or a combination thereof.

14. A method of claim 13 , wherein the Bradyrhizobium genus bacteria comprises Bradyrhizobium japonicum.

15. A method of claim 13 , wherein the Rhizobium genus bacteria comprises Rhizobium phaseoli, Rhizobium leguminosarum , or a combination thereof.

16. A method of claim 5 , wherein the agriculturally acceptable carrier comprises a dispersant, a surfactant, an additive, water, a thickener, an anti-caking agent, residue breakdown, a composting formulation, a granular application, diatomaceous earth, an oil, a coloring agent, a stabilizer, a preservative, a polymer, a coating, or a combination thereof.

17. A method of claim 5 , wherein the agriculturally acceptable carrier comprises vermiculite, charcoal, sugar factory carbonation press mud, rice husk, carboxymethyl cellulose, peat, perlite, fine sand, calcium carbonate, flour, alum, a starch, talc, polyvinyl pyrrolidone, or a combination thereof.

18. A method of claim 5 , wherein the inoculum is formulated as a seed coating formulation, a liquid formulation for application to plants or to a plant growth medium, or a solid formulation for application to plants or to a plant growth medium.

19. A method of claim 18 , wherein the seed coating formulation is an aqueous or oil-based solution for application to seeds.

20. A method of claim 18 , wherein the seed coating formulation is a powder or granular formulation for application to seeds.

21. A method of claim 18 , wherein the liquid formulation for application to plants or to a plant growth medium is in a concentrated formulation or a ready-to-use formulation.

22. A method of claim 18 , wherein the solid formulation for application to plants or to a plant growth medium is a granular formulation or a powder agent.

23. A method of claim 5 , wherein the inoculum further comprises a fertilizer, a micronutrient fertilizer material, an insecticide, a herbicide, a plant growth amendment, a fungicide, a molluscicide, an algicide, an additional bacterial inoculant, a fungal inoculant, or a combination thereof.

24. A method of claim 23 , wherein the insecticide comprises an organophosphate, a carbamate, a pyrethroid, an acaricide, an alkyl phthalate, boric acid, a borate, a fluoride, sulfur, a haloaromatic substituted urea, a hydrocarbon ester, a biologically-based insecticide, or a combination thereof.

25. A method of claim 23 , wherein the fungicide comprises a substituted benzene, a thiocarbamate, an ethylene bis dithiocarbamate, a thiophthalidamide, a copper compound, an organomercury compound, an organotin compound, a cadmium compound, anilazine, benomyl, cyclohexamide, dodine, etridiazole, iprodione, metlaxyl, thiamimefon, triforine, or a combination thereof.

26. A method of claim 23 , wherein the additional bacterial inoculant comprises a bacterial inoculant of the genus Rhizobium , a bacterial inoculant of the genus Bradyrhizobium , a bacterial inoculant of the genus Mesorhizobium , a bacterial inoculant of the genus Azorhizobium , a bacterial inoculant of the genus Allorhizobium , a bacterial inoculant of the genus Sinorhizobium , a bacterial inoculant of the genus Kluyvera , a bacterial inoculant of the genus Azotobacter , a bacterial inoculant of the genus Pseudomonas , a bacterial inoculant of the genus Azospirillium , a bacterial inoculant of the genus Bacillus , a bacterial inoculant of the genus Streptomyces , a bacterial inoculant of the genus Paenibacillus , a bacterial inoculant of the genus Paracoccus , a bacterial inoculant of the genus Enterobacter , a bacterial inoculant of the genus Alcaligenes , a bacterial inoculant of the genus Mycobacterium , a bacterial inoculant of the genus Trichoderma , a bacterial inoculant of the genus Gliocladium , a bacterial inoculant of the genus Glomus , a bacterial inoculant of the genus Klebsiella , or a combination thereof.

27. An inoculum for application to plants, plant seeds, or a plant growth medium, wherein the inoculum comprises an effective amount of a bacterial culture, an

Assignees

• Spogen Biotech Inc

Inventors

• Thompson Brian
• Thompson Katie
• Angle Brittany

Classifications

• C12R1/01

  Chemistry & Metallurgy · mapped topic

• A01N63/02

  Human Necessities · mapped topic

• C12R1/125

  Chemistry & Metallurgy · mapped topic

• A01N63/00 Primary

  Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03) · CPC title

• C12R1/075

  Chemistry & Metallurgy · mapped topic