Materials and methods for rapid and specific detection of cocaine
US-9804178-B2 · Oct 31, 2017 · US
Materials and methods for rapid and sensitive detection of small-molecule targets
US10550395B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10550395-B2 |
| Application number | US-201815947315-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 6, 2018 |
| Priority date | Apr 6, 2018 |
| Publication date | Feb 4, 2020 |
| Grant date | Feb 4, 2020 |
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- Title
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Abstract
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The subject invention provides methods, assays and products for detecting small-molecules in a sample, in particular, in both clinical and field settings. The method for detecting a small-molecule target in a sample comprises providing a sample, contacting the sample with an aptamer-based sensor selective for the small-molecule target, and sensitively and rapidly detecting the small-molecule target in the sample. Specifically, the method utilizes EATR-amplified small-molecule sensors based on cooperative binding split aptamers (CBSAs).
First claim
Opening claim text (preview).
What is claimed is: 1. A cooperative binding split aptamer (CBSA)-based enzyme-assisted target recycling (EATR)-amplified sensor comprising a CBSA, wherein the CBSA comprises SEQ ID NO:8 and SEQ ID NO:2. 2. The CBSA-based EATR-amplified sensor according to claim 1 , the CBSA being modified by addition of a reporter label. 3. The CBSA-based EATR-amplified sensor according to claim 2 , the reporter label being a fluorescent dye, a gold nanoparticle (AuNP), or a fluorescent dye and quencher pair. 4. A cooperative binding split aptamer (CBSA)-based enzyme-assisted target recycling (EATR)-amplified sensor comprising a CBSA, wherein the CBSA comprises SEQ ID NO:3 and SEQ ID NO:2. 5. A method for detecting dehydroisoandrosterone-3-sulfate (DIS) in a sample comprising contacting the sample with the CBSA-based EATR-amplified sensor of claim 4 . 6. The method according to claim 5 , the CBSA being recognized and cleaved by Exo III at a C3 spacer abasic site. 7. The method according to claim 5 , the sample being selected from blood, plasma, urine, tears, and saliva. 8. The method according to claim 5 , the method further comprising detecting a signal, the signal being a change in fluorescence intensity.
Assignees
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Classifications
Steroid hormones · CPC title
in screening processes · CPC title
- C12N15/115Primary
Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith {; Nucleic acids binding to non-nucleic acids, e.g. aptamers} · CPC title
Aptamers · CPC title
involving lipids, e.g. cholesterol {, lipoproteins, or their receptors (steroid hormones G01N33/743)} · CPC title
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- What does patent US10550395B2 cover?
- The subject invention provides methods, assays and products for detecting small-molecules in a sample, in particular, in both clinical and field settings. The method for detecting a small-molecule target in a sample comprises providing a sample, contacting the sample with an aptamer-based sensor selective for the small-molecule target, and sensitively and rapidly detecting the small-molecule ta…
- Who is the assignee on this patent?
- Xiao Yi, Yu Haixiang, The Florida International Univ Board Of Trustees
- What technology area does this patent fall under?
- Primary CPC classification C12N15/115. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Feb 04 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).