Composition for preparing tagatose and method for preparing tagatose from fructose

Patent metadata

Field	Value
Publication number	US-10544438-B2
Application number	US-201515521243-A
Country	US
Kind code	B2
Filing date	Oct 19, 2015
Priority date	Oct 22, 2014
Publication date	Jan 28, 2020
Grant date	Jan 28, 2020

Abstract

The present invention relates to a composition for preparing tagatose, wherein the composition is used for preparing tagatose from fructose and contains a protein including any one amino acid sequence of SEQ ID NOS: 1 to 7 or a microorganism expressing the protein. In addition, the present invention relates to a method for preparing tagatose from fructose, the method comprising a step of allowing the composition to react with fructose.

First claim

The invention claimed is:

1. A method of producing tagatose, the method comprising:
contacting fructose with a protein or a microorganism expressing the protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 7 such that the fructose is enzymatically converted to tagatose.

2. The method of producing tagatose according to claim 1 , wherein contacting is performed under at least one of the following conditions:
at a temperature between 50° C. and 90° C.,
at a pH between 6.0 and 10.0, and
at a fructose concentration of 5% (w/v) to 60% (w/v).

3. The method according to claim 2 , wherein the protein has amino acid sequence of SEQ ID NO: 7, wherein contacting is performed at a temperature between 50° C. and 80° C.

4. The method according to claim 2 , wherein the protein has an amino acid sequence of SEQ ID NO: 1, 3, 5, 6 or 7, wherein contacting is performed at a temperature between 60° C. and 80° C.

5. The method according to claim 2 , wherein the protein has an amino acid sequence of SEQ ID NO: 2 or 4, wherein contacting is performed at a temperature between 70° C. and 90° C.

6. The method according to claim 2 , wherein the protein has an amino acid sequence of SEQ ID NO: 1 or 6, wherein contacting is performed at a pH between 6.0 and 9.0.

7. The method according to claim 2 , wherein the protein has an amino acid sequence of SEQ ID NO: 4 or 7, wherein contacting is performed at a pH between 7.0 and 10.0.

8. The method according to claim 2 , wherein the protein has an amino acid sequence of SEQ ID NO: 1, 2, 3, or 5, wherein contacting is performed at a pH between 7.0 and 9.0.

9. The method according to claim 2 , wherein the protein has an amino acid sequence of SEQ ID NO: 4 or 7, wherein contacting is performed at a pH between 8.0 and 10.0.

10. The method according to claim 2 , wherein the protein has an amino acid sequence of SEQ ID NO: 6, wherein contacting is performed at a pH between 6.0 and 8.0.

11. The method of producing tagatose according to claim 1 , further comprising adding magnesium ions, zinc ions, nickel ions, cobalt ions, iron ions, manganese ions or a mixture of two or more of the foregoing.

12. The method according to claim 1 , further comprising converting fructose into tagatose through epimerization at carbon-4 position of fructose.

13. The method according to claim 1 , wherein in place of the protein to be used in the contacting step, a microorganism expressing protein having an amino acid sequence set forth in any one of SEQ ID NOs: 1 to 7 is used.

14. The method according to claim 1 , wherein the protein having an amino acid sequence set forth in SEQ ID NO: 1 is derived from Rhodothermus marinus.

15. The method according to claim 1 , wherein the protein having an amino acid sequence set forth in SEQ ID NO: 2 is derived from Thermotoga petrophila.

16. The method according to claim 1 , wherein the protein having an amino acid sequence set forth in SEQ ID NO: 3 is derived from Thermotoga lettingae.

17. The method according to claim 1 , wherein the protein having an amino acid sequence set forth in SEQ ID NO: 4 is derived from Thermoanaerobacter mathranii.

18. The method according to claim 1 , wherein the protein having an amino acid sequence set forth in SEQ ID NO: 5 is derived from Dictyoglomus turgidum.

19. The method according to claim 1 , wherein the protein having an amino acid sequence set forth in SEQ ID NO: 6 is derived from Thermoanaerobacterium xylanolyticum.

20. The method according to claim 1 , wherein the protein having an amino acid sequence set forth in SEQ ID NO: 7 is derived from Thermoanaerobacter siderophilus.

Assignees

• Cj Cheiljedang Corp

Inventors

• Kim Yang Hee
• Yang Sung Jae
• Lee Young Mi
• Park Il Hyang
• Kim Seong Bo
• Cho Hyun Kug
• Park Seung Won

Classifications

• C12Y501/03 Primary

  acting on carbohydrates and derivatives (5.1.3) · CPC title

• C12P19/24

  produced by the action of an isomerase, e.g. fructose · CPC title

• C12P19/02 Primary

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12N15/63

  Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression · CPC title

• C12N9/90 Primary

  Isomerases (5.) · CPC title