Matrix and composition for microbial culture of gram-positive bacteria

Patent metadata

Field	Value
Publication number	US-10538735-B2
Application number	US-201214368067-A
Country	US
Kind code	B2
Filing date	Dec 18, 2012
Priority date	Dec 22, 2011
Publication date	Jan 21, 2020
Grant date	Jan 21, 2020

Abstract

A composition for fermentation or microbial culture of gram-positive bacteria, includes fibril cellulose and at least one nutrient source including at least one carbon source, at least one nitrogen source, at least one phosphorus source, at least one mineral source and at least one trace element source.

First claim

The invention claimed is:

1. A three-dimensional matrix for microbial culture of gram-positive bacteria, said matrix comprising:
mechanically disintegrated nanofibrillar cellulose and at least one nutrient source comprising at least one carbon source, at least one nitrogen source, at least one phosphorus source, at least one mineral source and at least one trace element source, wherein the matrix is a hydrogel matrix comprising 0.1-3 wt % of nanofibrillar cellulose; and
living gram-positive bacteria cells suspended homogenously in the hydrogel matrix,
the hydrogel matrix having a yield stress sufficient to provide mechanical support for stabilizing the bacteria cells to uniformly grow and/or divide therein and for inhibiting sedimentation thereof, wherein the matrix has a liquid viscosity upon shearing such that the matrix is dispensible, pumpable, or injectable, the mechanically disintegrated nanofibrillar cellulose being formed using a refiner, a grinder, a homogenizer, a colloider, a friction grinder, an ultrasound-sonicator, a fluidizer, or a fibrillator.

2. The matrix according to claim 1 , wherein the nanofibrillar cellulose is selected from plant derived nanofibrillar cellulose and microbial nanofibrillar cellulose.

3. The matrix according to claim 1 , wherein the nanofibrillar cellulose is selected from native nanofibrillar cellulose and chemically modified nanofibrillar cellulose.

4. The matrix according to claim 1 , wherein the nanofibrillar cellulose is native ion-exchanged nanofibrillar cellulose.

5. The matrix of claim 1 , wherein the at least one nutrient source has limited solubility.

6. The matrix according to claim 1 , wherein the hydrogel matrix is a direct product of homogenization and fluidization of said nanofibrillar cellulose.

7. The matrix according to claim 1 , wherein the nanofibrillar cellulose is plant-derived nanofibrillar cellulose.

8. The matrix of claim 1 , wherein the nanofibrillar cellulose is configured to form a stable gel in a polar solvent.

9. A method for the manufacture of a three-dimensional matrix for microbial culture or fermentation of gram-positive bacteria, said method comprising:
providing living gram-positive bacteria;
mixing mechanically disintegrated nanofibrillar cellulose with water and at least one nutrient source comprising at least one carbon source, at least one nitrogen source, at least one phosphorus source, at least one mineral source and at least one trace element source to obtain the matrix, wherein the matrix comprises 0.1-3 wt % of nanofibrillar cellulose;
suspending the bacteria homogenously in the matrix,
wherein the matrix has a liquid viscosity upon shearing such that the matrix is dispensable, pumpable, or injectable, the mechanically disintegrated nanofibrillar cellulose being formed using a refiner, a grinder, a homogenizer, a colloider, a friction grinder, an ultrasound-sonicator, a fluidizer, or a fibrillator.

10. The method according to claim 9 , wherein the nanofibrillar cellulose is selected from plant derived nanofibrillar cellulose and microbial nanofibrillar cellulose.

11. The method according to claim 9 , wherein the nanofibrillar cellulose is selected from native nanofibrillar cellulose and chemically modified nanofibrillar cellulose.

12. The method according to claim 9 , wherein the nanofibrillar cellulose is native ion-exchanged nanofibrillar cellulose.

13. The method according to claim 9 , wherein the matrix is formed as a film, membrane, plate, or powder which is optionally dried.

14. The method according to claim 9 , wherein the matrix is sterilized.

15. A method for culturing or fermenting living gram-positive bacteria cells, wherein the living bacteria cells are incorporated in the hydrogel matrix of claim 1 to form a blend, which is then spread on a membrane, film, or plate and cultured or fermented.

16. The method according to claim 15 , wherein the culture or fermentation is carried out in a fermentation vessel.

17. The method according to claim 15 , wherein the culture or fermentation is carried out as bioleaching process.

Assignees

• Upm Kymmene Corp

Inventors

• Lauraeus Marko
• Laukkanen Antti

Classifications

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• C08L1/02

  Cellulose; Modified cellulose · CPC title

• C12N11/12

  Cellulose or derivatives thereof · CPC title

• C12N11/04

  entrapped within the carrier, e.g. gel or hollow fibres · CPC title

• C12N1/22 Primary

  Processes using, or culture media containing, cellulose or hydrolysates thereof · CPC title