Gas-fed fermentation reactors, systems and processes

Patent metadata

Field	Value
Publication number	US-10538730-B2
Application number	US-201916405587-A
Country	US
Kind code	B2
Filing date	May 7, 2019
Priority date	Jun 17, 2016
Publication date	Jan 21, 2020
Grant date	Jan 21, 2020

Abstract

Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

First claim

The invention claimed is:

1. A system for stimulating production of biomass comprising:
a loop reactor, the loop reactor including:
a gas/liquid separation vessel for separating a multi-phase mixture of a gas and a liquid culture medium into a gas phase and a liquid phase, the gas/liquid separation vessel including an outlet and an inlet;
a loop section including an inlet in fluid communication with the outlet of the gas/liquid separation vessel and an outlet in fluid communication with the inlet of the gas/liquid separation vessel, the loop section including a loop section centerline and sloped upward between the inlet of the loop section and the outlet of the loop section; and
a first non-vertical pressure reduction zone including a first pressure reduction device, the first non-vertical pressure reduction zone located between the inlet of the loop section and the outlet of the loop section, wherein a vertical distance between the loop section centerline at the inlet of the gas/liquid separation vessel and the loop section centerline at the inlet of the loop section is less than 8 meters.

2. The system of claim 1 , wherein the first pressure reduction device is selected from a flow control device and an expansion joint.

3. The system of claim 1 , further comprising a second pressure reduction zone downstream of the first non-vertical pressure reduction zone.

4. The system of claim 3 , wherein the second pressure reduction zone is a second non-vertical pressure reduction zone.

5. The system of claim 1 , wherein the vertical distance between the loop section centerline at the inlet of the gas/liquid separation vessel and the loop section centerline at the inlet of the loop section is less than 6 meters.

6. The system of claim 1 , wherein the vertical distance between the loop section centerline at the inlet of the gas/liquid separation vessel and the loop section centerline at the inlet of the loop section is less than 5 meters.

7. The system of claim 1 , wherein the loop reactor further comprises a desorption gas inlet, the desorption gas inlet located in a non-vertical portion of the loop section of the loop reactor.

8. The system of claim 1 , wherein the first pressure reduction device is a device that reduces pressure without relying upon a change in hydrostatic pressure.

9. The system of claim 1 , wherein the inlet of the gas/liquid separation vessel is spaced vertically from the outlet of the gas/liquid separation vessel.

10. The system of claim 1 , wherein the first pressure reduction device of the first non-vertical pressure reduction zone comprises a variable pressure reduction device.

11. The system of claim 1 , wherein the loop section includes a downward sloping section.

12. The system of claim 1 , wherein the loop reactor further comprises a desorption gas inlet upstream of the first non-vertical pressure reduction zone.

13. The system of claim 1 , wherein the inlet of the gas/liquid separation vessel is above the outlet of the gas/liquid separation vessel.

14. A system for stimulating production of biomass comprising:
a loop reactor, the loop reactor including:
a gas/liquid separation vessel for separating a multi-phase mixture of a gas and a liquid culture medium into a gas phase and a liquid phase, the gas/liquid separation vessel including an outlet and an inlet;
a loop section including an inlet in fluid communication with the outlet of the gas/liquid separation vessel and an outlet in fluid communication with the inlet of the gas/liquid separation vessel, the loop section including a loop section centerline; and
a first non-vertical pressure reduction zone including a first pressure reduction device including an eccentric reducer, the first non-vertical pressure reduction zone located between the inlet of the loop section and the outlet of the loop section, wherein a vertical distance between the loop section centerline at the inlet of the gas/liquid separation vessel and the loop section centerline at the inlet of the loop section is less than 8 meters.

15. The system of claim 14 , wherein the first pressure reduction device further comprises an eccentric expander downstream from the eccentric reducer.

16. The system of claim 15 , wherein the first pressure reduction device further comprises a control valve between the eccentric reducer and the eccentric expander.

17. The system of claim 14 , wherein the loop section includes a portion that slopes upward.

18. The system of claim 14 , wherein the loop section includes a portion that slopes downward.

19. A system for stimulating production of biomass comprising:
a loop reactor, the loop reactor including:
a gas/liquid separation vessel for separating a multi-phase mixture of a gas and a liquid culture medium into a gas phase and a liquid phase, the gas/liquid separation vessel including an outlet and an inlet;
a loop section including an inlet in fluid communication with the outlet of the gas/liquid separation vessel and an outlet in fluid communication with the inlet of the gas/liquid separation vessel, the inlet of the gas separation vessel spaced above the outlet of the gas/liquid separation vessel, the loop section including a loop section centerline and a portion that slopes upward in a direction extending from the loop section inlet to the loop section outlet; and
a first non-vertical pressure reduction zone including a control valve and an eccentric expander, the first non-vertical pressure reduction zone located between the inlet of the loop section and the outlet of the loop section, wherein a vertical distance between the loop section centerline at the inlet of the gas/liquid separation vessel and the loop section centerline at the inlet of the loop section is less than 8 meters.

20. The system of claim 19 , further comprising an eccentric reducer upstream of the eccentric expander.

21. The system of claim 19 , wherein the loop section includes a portion that slopes downward.

Assignees

• Calysta Inc

Inventors

• Nguyen Luan Thanh
• Johannessen Arild
• Aylen Graham Ian
• Silverman Joshua A

Classifications

• C12M21/02

  Photobioreactors (culturing algae A01G33/00, A01H4/001, C12N1/12) · CPC title

• C12M29/18 Primary

  External loop; Means for reintroduction of fermented biomass or liquid percolate (loop type reactors for chemical or physical processes B01J19/2435) · CPC title

• C12M29/20

  Degassing; Venting; Bubble traps (means for collection or storage of gas C12M23/36; gas collection apparatus for laboratory use B01L5/02) · CPC title

• C12N1/30

  having five or less carbon atoms · CPC title

• C12M45/04 Primary

  Phase separators; Separation of non fermentable material; Fractionation · CPC title