Method of degradation and inactivation of antibiotics in water by immobilized enzymes onto functionalized supports

Patent metadata

Field	Value
Publication number	US-10538443-B2
Application number	US-201515535825-A
Country	US
Kind code	B2
Filing date	Dec 14, 2015
Priority date	Dec 16, 2014
Publication date	Jan 21, 2020
Grant date	Jan 21, 2020

Abstract

The present invention relates to a method for degrading and inactivating at least one xenobiotic, the at least one xenobiotic being present in an aqueous medium. The method comprises the steps of (a) grafting at least one enzyme onto a solid support, (b) incubating the solid support with the at least one enzyme into the aqueous medium and (c) measuring the evolution of the concentration of the at least one xenobiotic. The method is remarkable in that the at least one enzyme is New-Dehli metallo-β-lactamase 1, a laccase extracted from Pleurotus ostreatus and/or a β-lactamase extracted from Pseudomonas aeruginosa and in that the solid support is a moving bed carrier.

First claim

The invention claimed is:

1. A method for degrading and inactivating at least one xenobiotic, the at least one xenobiotic being present in an aqueous medium, said method comprising:
grafting at least one enzyme onto a solid support;
incubating the solid support with the at least one enzyme in the aqueous medium; and
measuring the evolution of the concentration of the at least one xenobiotic,
wherein the at least one enzyme is at least one of a New-Delhi metallo-β-lactamase 1, a laccase extracted from Pleurotus ostreatus and a β-lactamase extracted from Pseudomonas aeruginosa , and wherein the solid support is a moving bed carrier.

2. The method according to claim 1 wherein the moving bed carrier is in high-density polyethylene.

3. The method according to claim 1 , wherein the moving bed carrier is a biochip carrier shaped like a disk with a three-dimensional checkering inside the disk and fins at the outside.

4. The method according to claim 1 , wherein the moving bed carrier is activated with at least one precursor prior to the grafting of at least one enzyme wherein the activation step is performed by surface treatment, with the at least one precursor of said moving bed carrier by one of cold plasma deposition at atmospheric pressure, and coating, the coating being an auto-polymerization process.

5. The method according to claim 4 , wherein the at least one precursor is one of polyglycidyl methacrylate, dopamine, maleic anhydride/vinyltrimethoxysilane, and dopamine acrylamide/vinyltrimethoxysilane.

6. The method according to claim 1 , wherein the method further comprises saturating the solid support by a microorganisms anti-adhesion layer after the grafting of the at least one enzyme onto the solid support.

7. The method according to claim 6 , wherein the microorganisms anti-adhesion layer comprises polyoxyethylene (20) sorbitan monolaurate.

8. The method according to claim 1 , wherein the at least one enzyme is chosen in a pure form.

9. The method according to claim 1 , wherein incubating the solid support with the at least one enzyme in the aqueous medium is performed at room temperature and under agitation, at one of 30, 50, 100, 200 and 300 revolutions per minute.

10. The method according to claim 1 , wherein measuring the evolution of the concentration of the at least one xenobiotic is performed by one of ultra-violet absorbance measurements and liquid chromatography and tandem mass spectrometry.

11. The method according to claim 1 , wherein the at least one xenobiotic is at least one antibiotic.

12. The method according to claim 11 , wherein the at least one antibiotic is an antibiotic chosen from one of the class of β-lactam antibiotics and the class of sulfonamide antibiotics.

13. The method according to claim 12 , wherein the antibiotic is one of amoxicillin and sulfamethoxazole.

14. A solid support, said support comprising:
at least one enzyme adapted for degrading and inactivating at least one xenobiotic, the at least one xenobiotic being present in an aqueous medium, wherein the at least one enzyme is at least one of a New-Delhi metallo-β-lactamase 1, a laccase extracted from Pleurotus ostreatus and a β-lactamase extracted from Pseudomonas aeruginosa , and wherein the solid support is a moving bed carrier.

Assignees

• Luxembourg Inst Science & Tech List

Inventors

• Bonot Sebastien
• Cauchie Henry-Michel

Classifications

• C02F3/342 Primary

  characterised by the enzymes used · CPC title

• C02F2101/30

  Organic compounds · CPC title

• C02F2209/00

  Controlling or monitoring parameters in water treatment · CPC title

• C02F2103/343

  from the pharmaceutical industry, e.g. containing antibiotics · CPC title

• C02F3/085 Primary

  Fluidized beds · CPC title