Manipulation of microfluidic droplets

Patent metadata

Field	Value
Publication number	US-10527529-B2
Application number	US-201715674074-A
Country	US
Kind code	B2
Filing date	Aug 10, 2017
Priority date	Mar 23, 2009
Publication date	Jan 7, 2020
Grant date	Jan 7, 2020

Abstract

The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.

First claim

What is claimed is:

1. A method for controlling droplet flow rate, the method comprising:
providing a first stream of droplets moving in a microfluidic channel at a first flow rate, the first stream of droplets comprising droplets of a first species;
providing a second stream of droplets moving within the microfluidic channel at a second flow rate, the second stream of droplets comprising droplets of a second species;
using a sensor to detect the first flow rate and the second flow rate;
receiving with a feedback controller the first and second flow rates; and
adjusting with the feedback controller the first and second flow rates relative to each other to cause the first flow rate to equal the second flow rate to intercalate the droplets of the first species and the droplets of the second species in a merge zone in the microfluidic channel, thereby causing merging of droplets of the first species with droplets of the second species.

2. The method of claim 1 , wherein the feedback controller adjusts the first flow rate or the second flow rate by adjusting a flow rate of one or more fluids.

3. The method of claim 1 , wherein the first and second species of droplets differ in size, color, refractive index, or extinction coefficient.

4. The method of claim 1 , wherein the first and second species of droplets contain a different biological, biochemical, or chemical entity.

5. The method of claim 1 , wherein the desired frequency ratio of the first plurality of droplets to the second plurality of droplets is selected from the group consisting of 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, and 1:10.

6. The method of claim 5 , wherein the desired frequency ratio of the first plurality of droplets to the second plurality of droplets is 1:1.

7. The method of claim 1 , wherein droplets of the first species have a maximum cross-sectional dimension of less than about 100 microns.

8. The method of claim 1 , wherein droplets of the second species have a maximum cross-sectional dimension of less than about 100 microns.

9. The method of claim 1 , wherein the two streams of droplets are initially flowed at the same flow rate, and wherein the feedback controller adjusts the flow rate of one or both streams of droplets to maintain the same flow rate.

10. The method of claim 1 , wherein the streams of droplets are driven by a drive infusion system selected from the group consisting of a pump, a pressure head, and an electro-kinetic drive.

11. The system of claim 10 , wherein drive infusion system is in communication with the feedback controller.

Assignees

• Bio Rad Laboratories

Inventors

• Miller Benjamin J
• Hutchison Brian
• Wilson Andrew
• Larson Jonathan William
• Zhong Qun
• Yurkovetsky Yevgeny
• Link Darren Roy
• Weary Mark

Classifications

• G01N27/44791

  Microapparatus (sample containers with integrated microfluidic structures B01L3/5027) · CPC title

• Y10T137/8593

  Systems · CPC title

• Y10T137/2572

  One inflow supplements another · CPC title

• G01N1/38 Primary

  Diluting, dispersing or mixing samples · CPC title

• B01L3/0241 Primary

  Drop counters; Drop formers · CPC title