Salmonella detection articles and methods of use

Patent metadata

Field	Value
Publication number	US-10526635-B2
Application number	US-201715438239-A
Country	US
Kind code	B2
Filing date	Feb 21, 2017
Priority date	May 20, 2011
Publication date	Jan 7, 2020
Grant date	Jan 7, 2020

Abstract

An article for detecting Salmonella microorganisms is provided. The article comprises a highly selective nutrient medium and a plurality of indicator systems. A method of using the article to detect Salmonella microorganisms is also provided.

First claim

The invention claimed is:

1. A culture device for detecting a target microorganism, comprising:
a selective growth medium that includes nutrients to facilitate the growth of the target microorganism, Cefsulodin, Nalidixic Acid, Streptomycin, Methicillin, and bile salts;
a first differential indicator system; and
a gelling agent; and
further comprising a nondifferential indicator system to indicate the presence of the target organism.

2. The culture device of claim 1 , wherein the first differential indicator system comprises a pH indicator.

3. The culture device of claim 1 , further comprising a second differential indicator system to indicate the presence of a nontarget microorganism.

4. The culture device of claim 3 , wherein the second differential indicator comprises a chromogenic β-galactosidase enzyme substrate.

5. The culture device of claim 1 , wherein the selective growth medium comprises a dry, rehydratable selective growth medium, wherein the gelling agent comprises a dry, cold water-soluble gelling agent.

6. The culture device of claim 5 , wherein the culture device comprises a thin film culture device.

7. The culture device of claim 1 , wherein the culture device further comprises a third differential indicator system.

8. The culture device of claim 3 , wherein the culture device further comprises a third differential indicator system.

9. A method of using the culture device of claim 1 , comprising contacting the culture device with liquid sample to form an inoculated culture device.

10. The method of claim 9 , further comprising incubating the inoculated culture device for a first period of time.

11. The method of claim 10 , further comprising observing the growth medium for an indication of a possible presence of a target microorganism in the sample.

12. The method of claim 11 , further comprising contacting the growth medium with a second differential indicator system.

13. The method of claim 12 , further comprising contacting the growth medium with a third differential indicator system.

14. The method of claim 13 , further comprising detecting the presence of a target microorganism in the sample, wherein the target microorganism is Salmonella.

15. A method of using the culture device of claim 3 , comprising contacting the culture device with liquid sample to form an inoculated culture device.

16. The method of claim 15 , further comprising incubating the inoculated culture device for a first period of time.

17. The method of claim 16 , further comprising observing the growth medium for an indication of a possible presence of a target microorganism in the sample.

18. The method of claim 17 , further comprising contacting the growth medium with a third differential indicator system.

19. The method of claim 18 , further comprising detecting the presence of a target microorganism in the sample, wherein the target microorganism is Salmonella.

20. The method of claim 15 , further comprising detecting the presence of a target microorganism in the sample, wherein the target microorganism is Salmonella.

Assignees

• 3M Innovative Properties Co

Inventors

• Mach Patrick A
• Celt Mara S

Classifications

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title

• C12Q1/045

  Culture media therefor · CPC title

• C12Q1/04 Primary

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• C12Q1/10

  Enterobacteria · CPC title

• C12Q1/34

  involving hydrolase · CPC title