Synthetic production of circular dna vectors
US-2024409975-A1 · Dec 12, 2024 · US
Enzyme-mediated assimilation of DNA-functionalized single-walled carbon nanotubes (SWNTs)
US10526628B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10526628-B2 |
| Application number | US-201113267002-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 6, 2011 |
| Priority date | Oct 6, 2010 |
| Publication date | Jan 7, 2020 |
| Grant date | Jan 7, 2020 |
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- Title
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Abstract
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Select embodiments of the present invention employ biological means to direct assemble CNT-based nanostructures, allowing for scaling to macrostructures for manufacture. In select embodiments of the present invention, a method is provided for assembling DNA-functionalized SWNTs by phosphodiester bonding catalyzed by ssDNA-ligase to form macroscopic CNT aggregates.
First claim
Opening claim text (preview).
We claim: 1. A method for aggregating CNTs to permit scaling for manufacture of macro-scale items, comprising: functionalizing SWNTs; performing ligation reactions using an adenosine triphosphate (ATP)-dependent, thermostable ssDNA-ligase, wherein said ligation reactions are initiated in a heated water bath; and terminating said reactions after a pre-specified period by cooling said water bath to room temperature; and wherein said SWNTs are functionalized with single-stranded 30-mer polythymine oligonucleotides (d(T) 30 ) by ultra-sonication. 2. The method of claim 1 centrifuging said SWNTs to remove insoluble CNTs. 3. The method of claim 1 removing potentially unbound said ssDNA. 4. The method of claim 1 performing ligation reactions using: purified DNA-functionalized SWNTs, 10× ligase buffer, 1 mM ATP, said ssDNA-ligase, and molecular-grade water. 5. The method of claim 4 initiating said reactions by incubating in a water bath at a temperature of approximately 30° C. to approximately 100° C. 6. The method of claim 4 initiating said reactions by incubating in a water bath at a temperature of approximately 60° C. 7. The method of claim 5 terminating said reactions after approximately one hour by cooling to room temperature.
Assignees
Inventors
Classifications
- C12P19/34Primary
Polynucleotides, e.g. nucleic acids, oligoribonucleotides · CPC title
Single-walled nanotubes · CPC title
Derivatisation; Solubilisation; Dispersion in solvents · CPC title
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Frequently asked questions
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- What does patent US10526628B2 cover?
- Select embodiments of the present invention employ biological means to direct assemble CNT-based nanostructures, allowing for scaling to macrostructures for manufacture. In select embodiments of the present invention, a method is provided for assembling DNA-functionalized SWNTs by phosphodiester bonding catalyzed by ssDNA-ligase to form macroscopic CNT aggregates.
- Who is the assignee on this patent?
- Arnett Clint M, Marsh Charles P, Han Jae Hee, and 4 more
- What technology area does this patent fall under?
- Primary CPC classification C12P19/34. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 07 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).